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    Adipokine, gut and thyroid hormone responses to probiotic application in chukar partridges (Alectoris chukar) exposed to heat stress
    (Akademiai Kiado Zrt, 2021) Bayraktar, Bulent; Tekce, Emre; Kaya, Hacer; Gurbuz, Ahmet Burak; Dirican, Ebubekir; Korkmaz, Serol; Atalay, Banu
    The aim of this study was to investigate the effect of Lactobacillus reuteri E81 (LRE) probiotic supplementation on heat stress responses in chukar partridges (Alectoris chukar). The birds were divided into two groups, one of which was exposed to heat stress (HS). Within each group, four subgroups, each including 64 birds, were created for the three treatment doses (200, 400 or 600 mg/kg) of LRE and the control. The experiment was started with day-old birds, kept at a temperature of 25 degrees C or 37 degrees C. After a 7-day adjustment period, the LRE supplementation lasted for 35 days. The levels of different adipokines, including visfatin (VF), adiponectin (ADP), chemerin (CHEM), as well as the concentration of plasma citrulline (CIT) and the levels of thyroid hormones (T3 and T4) and thyroid-stimulating hormone (TSH) in the blood were measured at 21 and 42 days of age. A significant correlation (P < 0.01) was found between LRE supplementation and the decrease in serum VF, ADP, CIT, T3 and T4 levels in partridges exposed to HS. On the other hand, no significant relationship was found between LRE supplementation and the serum CHEM and TSH levels (P > 0.05). We concluded that the addition of 600 mg/kg LRE is beneficial in preventing intestinal damage and inflammation provoked by HS.
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    The cytogenetic effects of the aqueous extracts of migratory locust ( Locusta migratoria L.) in vitro
    (Sage Publications Inc, 2014) Turkez, Hasan; Incekara, Umit; Guner, Adem; Aydin, Elanur; Dirican, Ebubekir; Togar, Basak
    One of the useful and most commonly cultivated commercially species, migratory locust (Locusta migratoria; Orthoptera), was investigated in light of genotoxic damage potentials. For this aim, we evaluated the genotoxic potentials of water soluble extracts of L. migratoria on cultured human blood cells. The micronucleus, sister chromatid exchange and structural chromosome aberration assays were applied to assess DNA and chromosomal damage produced by aqueous extracts in vitro. The extracts were added to the cultures at different concentrations ranging from 0 to 1000mg/L. Our results indicated that these extracts did not exhibit genotoxicity at tested concentrations. We conclude that this in vitro approach for biomonitoring genotoxicity assessment is useful for comparing the potential health risks of edible insects.
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    Genotoxic effect of beryllium chloride and protective role of crataegus oxyacantha L. (Hawthorn) fruit extract in vitro
    (2012) Dirican, Ebubekir; Türkez, Hasan
    Beryllium (Be) is used in various industries (electronics, energy, automotive, and aerospace applications); however, there is very scarce data on its genotoxicity. On the other hand, Crataegus oxyacantha is a thorny tree that thrives in hedgerows and fields in the temperate regions of Europe and the British Isles. Besides, C. oxyacantha berry extract is known as one of the most powerful antioxidants. In this study, antigenotoxic effects of C. oxyacantha against Be-induced chromosomal aberrations (CA), sister chromatid exchanges (SCE) and micronucleus (MN) formation in human lymphocytes were investigated. All cultures were treated with 120 mg/L beryllium chloride (BeCl2) for 48 h. Three different concentrations (25, 50 and 100 mg/L) of C. oxyacantha fruit extract was added to the BeCl2-treated cultures. In BeCl 2 and C. oxyacantha combination, the frequency of abnormal cells and CA/cell significantly decreased compared to BeCl2 alone. Statistically significant reduction was also observed in SCE/cell and MN frequencies in all the treatments. These results illustrated anticlastogenic and antimutagenic effects of C. oxyacantha fruit extract against BeCl 2-induced genetic damage. On the other hand, BeCl2 + C. oxyacantha treatments induced significant reduction in the mitotic index, with regard to BeCl2 treatment alone. Based on the results of this study, it may be concluded that dose-controlled C. oxyacantha diet may play a protective role in the process of BeCl2 mutagenesis and/or carcinogenesis.
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    Öğe
    GENOTOXIC EFFECT OF BERYLLIUM CHLORIDE AND PROTECTIVE ROLE OF Crataegus Oxyacantha L. (HAWTHORN) FRUIT EXTRACT IN VITRO
    (Parlar Scientific Publications (P S P), 2012) Dirican, Ebubekir; Tuerkez, Hasan
    Beryllium (Be) is used in various industries (electronics, energy, automotive, and aerospace applications); however, there is very scarce data on its genotoxicity. On the other hand, Crataegus oxyacantha is a thorny tree that thrives in hedgerows and fields in the temperate regions of Europe and the British Isles. Besides, C. oxyacantha berry extract is known as one of the most powerful antioxidants. In this study, antigenotoxic effects of C. oxyacantha against Be-induced chromosomal aberrations (CA), sister chromatid exchanges (SCE) and micronucleus (MN) formation in human lymphocytes were investigated. All cultures were treated with 120 mg/L beryllium chloride (BeCl2) for 48 h. Three different concentrations (25, 50 and 100 mg/L) of C. oxyacantha fruit extract was added to the BeCl2-treated cultures. In BeCl2 and C. oxyacantha combination, the frequency of abnormal cells and CA/cell significantly decreased compared to BeCl2 alone. Statistically significant reduction was also observed in SCE/cell and MN frequencies in all the treatments. These results illustrated anticlastogenic and antimutagenic effects of C. oxyacantha fruit extract against BeCl2-induced genetic damage. On the other hand, BeCl2 + C. oxyacantha treatments induced significant reduction in the mitotic index, with regard to BeCl2 treatment alone. Based on the results of this study, it may be concluded that dose-controlled C. oxyacantha diet may play a protective role in the process of BeCl2 mutagenesis and/or carcinogenesis.
  • Küçük Resim
    Öğe
    In vitro studies on protective effect of Glycyrrhiza glabra root extracts against cadmium-induced genetic and oxidative damage in human lymphocytes
    (Springer, 2014) Dirican, Ebubekir; Turkez, Hasan
    Cadmium is a modern environmental contaminant that is toxic and carcinogenic. Glycyrrhiza glabra is a traditional medicinal herb which grows in the various parts of the World. Recent studies demonstrated that G. glabra has antifungal, antimicrobial, antioxidant, and powerful antiinflammatory features. The purpose of this study was to investigate the genetic safety of extracts from G. glabra and its effects on cadmium (as CdCl2) induced genotoxicity. Therefore we evaluated the capability of G. glabra extract to inhibit the rate of micronucleus (MN), sister chromatid exchange (SCE) formations induced by CdCl2. Moreover, to assess the effects of G. glabra on cell viability and oxidative status, we performed 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) and total antioxidant capacity (TAC) assays. Our results showed that there were significant increases (P\0.05) in both SCE and MN frequencies of cultures treated with CdCl2 (5 ppm) as compared to controls. However, co-application of G. glabra extract (5, 10 and 20 ppm) and CdCl2 resulted in decreases of MN and SCE rates as compared to the group treated with CdCl2 alone. Again, the results of MTT and TAC assays clearly indicated dose dependent ameliorative effects of G. glabra extracts against CdCl2 toxicity. In conclusion, this study demonstrated for the first time that G. glabra extracts provided increased resistance of DNA against CdCl2 induced genetic and oxidative damage in human lymphocytes. So, the risk on target tissues of CdCl2 could be reduced and ensured early recovery from its toxicity.
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    Thymbra spicata l. modulates chromium (iii) chloride-induced genetic and oxidative damage in vitro
    (2012) Dirican, Ebubekir; Türkez, Hasan
    Chromium is a toxic heavy metal used in various industries and leads to environmental pollution due to improper handling. Cr exhibits oxidative stress mediated genotoxic damage although the mechanism of cellular damage caused by Cr has not been fully elucidated. On the other hand, Thymbra spicata L., a member of the Lamiaceae family, was recently investigated popularly of biological roles; mainly antioxidant and antimicrobial activities. However, there is very scarce data on the cytogenetic effects of Thyme species. Therefore, in this study, we aimed to determine whether T. spicata extracts conferred a protection against Chromium (III) chloride (CrCl3)-induced genotoxic and oxidative damage in vitro. For this aim, we determined sister chromatid exchange (SCE) rates and main antioxidant enzyme activities including catalase (CAT) superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) in CrCl 3 (25 ?M) and T. spicata extracts (TSE) (25, 50, 100 ?M) treated human whole blood cultures (n=3) for 72h. T. spicata extracts at tested concentrations did not exhibit any negative effects on above studied parameters in culture tubes. Besides, the results of the present study indicated that the increases of SCE frequencies and the decreases of antioxidant enzyme activities by CrCl3 were minimized by the application of the T. spicata extracts. Our results firstly suggest that T. spicata augments the antioxidants defense against CrCl3 induced toxicity. Based on the results of this study, it may be concluded that dose controlled T. spicata diet may play a protective role in the process of Cr mutagenesis and/or carcinogenesis.
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    THYMBRA SPICATA L. MODULATES CHROMIUM (III) CHLORIDE-INDUCED GENETIC AND OXIDATIVE DAMAGE IN VITRO
    (Parlar Scientific Publications (P S P), 2012) Dirican, Ebubekir; Turkez, Hasan
    Chromium is a toxic heavy metal used in various industries and leads to environmental pollution due to improper handling. Cr exhibits oxidative stress mediated genotoxic damage although the mechanism of cellular damage caused by Cr has not been fully elucidated. On the other hand, Thymbra spicata L., a member of the Lamiaceae family, was recently investigated popularly of biological roles; mainly antioxidant and antimicrobial activities. However, there is very scarce data on the cytogenetic effects of Thyme species. Therefore, in this study, we aimed to determine whether T. spicata extracts conferred a protection against Chromium (III) chloride (CrCl3)-induced genotoxic and oxidative damage in vitro. For this aim, we determined sister chromatid exchange (SCE) rates and main antioxidant enzyme activities including catalase (CAT) superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) in CrC3l (25 mu M) and T. spicata extracts (TSE) (25, 50, 100 mu M) treated human whole blood cultures (n=3) for 72h. T. spicata extracts at tested concentrations did not exhibit any negative effects on above studied parameters in culture tubes. Besides, the results of the present study indicated that the increases of SCE frequencies and the decreases of antioxidant enzyme activities by CrCl3 were minimized by the application of the T. spicata extracts. Our results firstly suggest that T. spicata augments the antioxidants defense against CrCl3 induced toxicity. Based on the results of this study, it may be concluded that dose controlled T. spicata diet may play a protective role in the process of Cr mutagenesis and/or carcinogenesis.