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  • [ N/A ]
    Öğe
    Effects of Interactions among Gene Polymorphisms of the Renin-Angiotensin-Aldosterone System on Hypertension in Turkish People from Southeast Anatolia
    (Sciendo, 2019) Arpaci, Abdullah; Urhan-Kucuk, Meral; Bayramoglu, Aysegul; Guler, Halil Ibrahim; Ecevit, Hasret; Suner, Arif; Karakas-Celik, Sevim
    Introduction: Hypertension (HT) is characterized by high blood pressure. The reninangiotensin-aldosterone system (RAAS) plays a crucial role in blood pressure (BP) regulation by maintaining vascular tone and the water-sodium balance. We aimed to investigate whether there is any relation between AGT (M235T), ACE (I/D), and AGTR1 (A1166C) genetic polymorphisms and hypertension among Turkish people from Southeast Anatolia. Method and Results: A total of 210 individuals, consisting of 102 healthy controls and 108 patients with essential hypertension admitted to the Cardiology Department of the Adiyaman University Research and Application Hospital were included the study. DNA isolation was performed from the blood samples via commercial kit. Genotype determination was determined using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. Statistically significant differences were found between the control and patient groups in terms of genotype distribution and allelic frequencies of ACE I/D polymorphisms. Significant differences were found in the frequencies of ICM, DCM, DCT, DAT between the patient and control groups. Conclusions: In this study, we found a significant association of ACE I/D polymorphism with HT, and we showed that the I allele can increase the risk of HT in Turkish people from Southeast Anatolia. Although we did not find any association between independent AGT M235T and AGT1R A1166C polymorphisms and HT, we observed that the DCM, DCT, and DAT haplotypes of ACE/AGT1R/AGT polymorphisms reduced the risk of hypertension, while the ICM haplotype increased it.
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    Öğe
    Enzyme activity and genetic polymorphisms in patients with type II diabetes mellitus
    (Wroclaw Medical Univ, 2020) Arpaci, Abdullah; Yalin, Serap; Ecevit, Hasret; Comelekoglu, Ulku; Mete, Turkan
    Background. Diabetes mellitus (DM) has become more and more common and has a high morbidity and mortality rate worldwide. It is a multifactorial chronic disease affected by both genetic and environmental factors. Objectives. To evaluate the association between antioxidant enzyme activities and their genetic variations and the level of malondialdehyde (MDA) in type II diabetes patients living in the Adlyaman province in the southeast part of Turkey. Material and methods. One hundred patients diagnosed with type II DM (T2DM) and 100 healthy controls were included in the study. Malondialdehyde levels and antioxidant enzyme activities were measured spectrophometrically. DNA isolation was performed and genotyping was carried out using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results. Our results revealed no significant differences in genotype distributions and allele frequencies of all polymorphisms between groups (p > 0.05). Significantly elevated MDA levels and a significant reduction in catalase (CAT) and paraoxonase (PON) enzyme activities were observed in patients compared to the control group in terms of study groups and genetic variations (p < 0.05). Moreover, CAT activity was reduced in TT genotype in terms of CAT -262 C/T polymorphism in patients (p < 0.05). Paraoxonase activity was observed to be lower in MM genotype in both groups (p < 0.05). Conclusions. CAT-262 C/T polymorphism may be one of the factors that lead to severe clinical situation in DM. Our results suggest that TT genotype may be more prone to lipid peroxidation.
  • Küçük Resim
    Öğe
    Investigation of antioxidant effect of silibinin molecule on U-2 OS cells induced by hydrogen peroxide
    (2022) Gündüz, Kübra; Ecevit, Hasret; Küçük, Meral Urhan; Dırıcan, Emre
    Aim: Silibinin is one of the active constituent of silymarin, an extract of the Silybum\rmarianum L. seeds. There are previous studies that have reported the antioxidant, antiinflammatory, anti-apoptotic and anti-carcinogenic effects of silibinin. However, the antioxidant effect of silibinin on bone cancer have been not evaluated before. In our study,\rwe aimed to evaluate the effect of silibinin on U-2 OS cell proliferation and oxidative stress\rparameters catalase enzyme activity and MDA level.\rMaterials and Methods: U-2 OS cells were exposed to H2O2 (0-800 µM H2O2, for\r24 and 48 hours) to form an oxidative damaged cell model. Moreover, the cells were exposed to silibinin (0-100 µM) for 24 hours. Malondialdehyde (MDA) level and antioxidant\renzyme catalase (CAT) activity were determined by spectrophotometric analysis.\rResults: CAT activity and MDA level were observed to be higher in both H2O2 exposure\rgroups compared to control group (p<0.05). Moreover, CAT activity and MDA level were\robserved to be lower in 500 µM H2O2+silibinin groups in comparation with 500 µM H2O2\rgroup (p<0.05). Similarly, MDA level was determined to be lower in 650 µM H2O2+10\rµM silibinin group in comparation to 650 µM H2O2 group (p<0.05).\rConclusion: In conclusion, it was observed that silibinin molecule has anti-proliferative\reffect and decreases catalase enzyme activity and MDA level in a concentration dependent\rmanner.
  • Küçük Resim
    Öğe
    Orak hücreli anemili hastalarda, oksidatif stres belirteci olarak 8-OHDG (8-hidroksi deoksi guanozin), MDA(malonil dialdehit) ve PC (protein karbonil) düzeylerinin araştırılması
    (Hatay Mustafa Kemal Üniversitesi, 2013) Ecevit, Hasret; Öztürk, Oktay Hasan
    Orak hücre anemisi, Çukurova bölgesinde yaygın görülen, vasküler seviyede kronik inflamasyona sebebiyet veren mikrovasküler hipoksik ve reperfüzyon süreci ile karakterize olan konjenital bir hemoglobinopatidir. Bu çalışmada, oksidatif stres belirteci olarak serum 8-hidroksi deoksi guanozin (8-OHdG), malonildialdehit (MDA) ve protein karbonil (PC) düzeylerinin belirlenmesi amaçlandı. Çalışmaya OHA hastası olan 18 yaş altı hastalar (n=45) ve sağlıklı bireyler (n=38) dahil edildi. Serum örneklerinden 8-OHdG ELISA yöntemiyle, MDA ve PC düzeyleri hem ELISA hem spektrofotometrik yöntemle çalışıldı. OHA grubu ve kontrol grubu karşılaştırıldığında, OHA grubunda ELISA yöntemiyle ölçülen 8-OHdG (p=0,0243) düzeyleri istatistiksel olarak yüksek tespit edildi. PC ve MDA düzeyleri manuel spektrofotometrik yöntem ile çalışıldığında OHA grubunda istatistiksel olarak yüksek tespit edildi (p<0,0001). PC ve MDA düzeyleri ELISA yöntemiyle çalışıldığında ise OHA ve kontrol grubu arasında istatistiksel fark gözlenmedi (p>0,05). Orak hücreli anemili hastalarda, DNA?nın yapısındaki guaninin oksidatif modifikasyonu sonucu oluşan 8-OHdG, lipit peroksidasyon ürünü olan MDA ve protein oksidasyonu sonucu oluşan PC düzeylerinin yüksek olması, bu parametrelerin artmış oksidatif stres ile ilişkisine dayanarak OHA?nın patofizyolojisinde önemli bir rol oynadığını göstermektedir.
  • Küçük Resim
    Öğe
    The Role of Oxidative Stress in Apoptosis and Cell Proliferation of Human Bronchial Epithelial Cells
    (Pleiades Publishing Inc, 2021) Ecevit, Hasret; Urhan-Kucuk, Meral; Uluca, Haluk; Tap, Duygu; Arpaci, Abdullah
    Oxidative stress is an important pathophysiological factor in chronic respiratory diseases. Our study aimed at elucidating through which pathway oxidative stress-mediated apoptosis occurs at the gene expression level under oxidative stress in the human bronchial epithelial cell line BEAS-2B. Suitable doses and time period were detected by exposing BEAS-2B cells to hydrogen peroxide (H2O2) at different doses and time periods, and the oxidative-damaged cell culture model was designed. The treatment and control groups were compared in terms of gene expression levels determined by Quantitative Real Time Polymerase Chain Reaction. The oxidative-damaged cell model was confirmed by the spectrophotometric measurement of malondialdehyde and catalase activity (p < 0.05). Caspase-3, caspase-9, bax, and bak gene expression levels increased significantly in the treatment groups compared to the control group (p < 0.05). There were not any significant differences between the groups in terms of caspase-8, Bcl-2, and bik (p > 0.05). p53 and p21 gene expression levels were found to be significantly higher in the treatment groups (p < 0.05). H2O2-induced oxidative stress, induced apoptosis through the intrinsic pathway at gene expression level in the bronchial epithelial BEAS-2B cells was observed.