The Role of Oxidative Stress in Apoptosis and Cell Proliferation of Human Bronchial Epithelial Cells
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Dosyalar
Tarih
2021
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Pleiades Publishing Inc
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Oxidative stress is an important pathophysiological factor in chronic respiratory diseases. Our study aimed at elucidating through which pathway oxidative stress-mediated apoptosis occurs at the gene expression level under oxidative stress in the human bronchial epithelial cell line BEAS-2B. Suitable doses and time period were detected by exposing BEAS-2B cells to hydrogen peroxide (H2O2) at different doses and time periods, and the oxidative-damaged cell culture model was designed. The treatment and control groups were compared in terms of gene expression levels determined by Quantitative Real Time Polymerase Chain Reaction. The oxidative-damaged cell model was confirmed by the spectrophotometric measurement of malondialdehyde and catalase activity (p < 0.05). Caspase-3, caspase-9, bax, and bak gene expression levels increased significantly in the treatment groups compared to the control group (p < 0.05). There were not any significant differences between the groups in terms of caspase-8, Bcl-2, and bik (p > 0.05). p53 and p21 gene expression levels were found to be significantly higher in the treatment groups (p < 0.05). H2O2-induced oxidative stress, induced apoptosis through the intrinsic pathway at gene expression level in the bronchial epithelial BEAS-2B cells was observed.
Açıklama
Anahtar Kelimeler
apoptosis, BEAS-2B, cell proliferation, oxidative stress, reactive oxygen species
Kaynak
Cytology and Genetics
WoS Q Değeri
Q4
Scopus Q Değeri
Q3
Cilt
55
Sayı
3
