The Role of Oxidative Stress in Apoptosis and Cell Proliferation of Human Bronchial Epithelial Cells

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dc.contributor.authorEcevit, Hasret
dc.contributor.authorUrhan-Kucuk, Meral
dc.contributor.authorUluca, Haluk
dc.contributor.authorTap, Duygu
dc.contributor.authorArpaci, Abdullah
dc.date.accessioned2024-09-18T20:02:34Z
dc.date.available2024-09-18T20:02:34Z
dc.date.issued2021
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractOxidative stress is an important pathophysiological factor in chronic respiratory diseases. Our study aimed at elucidating through which pathway oxidative stress-mediated apoptosis occurs at the gene expression level under oxidative stress in the human bronchial epithelial cell line BEAS-2B. Suitable doses and time period were detected by exposing BEAS-2B cells to hydrogen peroxide (H2O2) at different doses and time periods, and the oxidative-damaged cell culture model was designed. The treatment and control groups were compared in terms of gene expression levels determined by Quantitative Real Time Polymerase Chain Reaction. The oxidative-damaged cell model was confirmed by the spectrophotometric measurement of malondialdehyde and catalase activity (p < 0.05). Caspase-3, caspase-9, bax, and bak gene expression levels increased significantly in the treatment groups compared to the control group (p < 0.05). There were not any significant differences between the groups in terms of caspase-8, Bcl-2, and bik (p > 0.05). p53 and p21 gene expression levels were found to be significantly higher in the treatment groups (p < 0.05). H2O2-induced oxidative stress, induced apoptosis through the intrinsic pathway at gene expression level in the bronchial epithelial BEAS-2B cells was observed.en_US
dc.description.sponsorshipHatay Mustafa Kemal University Scientific Research Projects Unit Hatay, Turkey [16745]en_US
dc.description.sponsorshipThis study is supported by Hatay Mustafa Kemal University Scientific Research Projects Unit (Project no. 16745) Hatay, Turkey.en_US
dc.identifier.doi10.3103/S0095452721030026
dc.identifier.endpage289en_US
dc.identifier.issn0095-4527
dc.identifier.issn1934-9440
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85107134866en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage283en_US
dc.identifier.urihttps://doi.org/10.3103/S0095452721030026
dc.identifier.urihttps://hdl.handle.net/20.500.12483/7864
dc.identifier.volume55en_US
dc.identifier.wosWOS:000657840200009en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherPleiades Publishing Incen_US
dc.relation.ispartofCytology and Geneticsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectapoptosisen_US
dc.subjectBEAS-2Ben_US
dc.subjectcell proliferationen_US
dc.subjectoxidative stressen_US
dc.subjectreactive oxygen speciesen_US
dc.titleThe Role of Oxidative Stress in Apoptosis and Cell Proliferation of Human Bronchial Epithelial Cellsen_US
dc.typeArticleen_US

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