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  • Küçük Resim
    Öğe
    Effective antibacterial and antioxidant properties of methanolic extract of Laurus nobilis seed oil
    (Triveni Enterprises, 2010) Ozcan, Birgul; Esen, Mari; Sangun, M. Kemal; Coleri, Arzu; Caliskan, Mahmut
    This study was carried out to determine the in vitro antimicrobial and antioxidant activities of the essential oil, seed oil, and methanolic extract of seed oil obtained from Laurus nobilis L. (Lauraceae). The methanolic extract of seed oil exhibited more effective antibacterial activity comparing to essential oil and seed oil. GC-MS analyses of the essential oil resulted in the identification of 25 compounds. 1.8-Cineol (44.72%), a-Terpinyl acetate (12.95%), Sabinene (12.82%) were the main components. The fatty acid composition was characterized with the high content of linoleic acid (40.79%) and lauric acid (38.08%). The 50% (IC50) inhibition activity of the essential oil on the free radical DPPH was determined as 94.655 mgml(-1), whereas IC50 value of methanolic extract of seed oil was found unstable. In the case of the linoleic acid system, oxidation of linoleic acid was inhibited by essential oil and methanolic extract of seed oil, which showed 64.28 and 88.76% inhibition, respectively The inhibition value of the methanolic extract of seed oil was quite close to the synthetic antioxidant BHT, 92.46% inhibition.
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    Öğe
    In vitro antimicrobial and antioxidant activities of various extracts of salvia microstegia (boiss.) et. bal. from antakya, Turkey
    (2009) Ozcan, Birgul; Esen, Mari; Coleri, Arzu; Yolcu, Hikmet; Caliskan, Mahmut
    The present study was performed to evaluate the in vitro antimicrobial and antioxidant properties of various extracts (hexane, dichloromethane, methanol/chloroform, methanol/water and direct methanol) from Salvia microstegia (Boiss.) Et. Bal., a member of Lamiaceae family. Direct methanol extracts of S. microstegia exhibited more effective antibacterial activities on the Gram-negative and Gram-positive bacteria. None of the extracts exhibited any antifungal activities on the Candida albicans ATCC 10231. The total phenolic components of S. microstegia was found 103.64 mg/g gallic acid equivalent. The antioxidant activity of the methanolic extract of S. microstegia was examined with two complementary test systems; 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ß-carotene-linoleic acid assays. The 50% (IC50) inhibition activity of the methanolic extract of S. microstegia on the free radical DPPH was determined as 7.63 mg/ml. In the case of the linoleic acid system, the methanolic extract of S. microstegia showed 79.92% inhibition, this value is quite close to the value of synthetic antioxidant reagent, 92.46%. © by PSP Volume 18-No 5a. 2009.
  • [ N/A ]
    Öğe
    IN VITRO ANTIMICROBIAL AND ANTIOXIDANT ACTIVITIES OF VARIOUS EXTRACTS OF Salvia microstegia (BOISS.) ET. BAL. FROM ANTAKYA, TURKEY
    (Parlar Scientific Publications (P S P), 2009) Ozcan, Birgul; Esen, Mari; Coleri, Arzu; Yolcu, Hikmet; Caliskan, Mahmut
    The present study was performed to evaluate the in vitro antimicrobial and antioxidant properties of various extracts (hexane, dichloromethane, methanol/chloroform, methanol/water and direct methanol) from Salvia microstegia (Boiss.) Et. Bal., a member of Lamiaceae family. Direct methanol extracts of S. microstegia exhibited more effective antibacterial activities on the Gram-negative and Gram-positive bacteria. None of the extracts exhibited any antifungal activities on the Candida albicans ATCC 10231. The total phenolic components of S. microstegia was found 103.64 mg/g gallic acid equivalent. The antioxidant activity of the methanolic extract of S. microstegia was examined with two complementary test systems; 2,2-diphenyl-1-picrylhydrazyl (DPPH) and beta-carotene-linoleic acid assays. The 50% (IC50) inhibition activity of the methanolic extract of S. microstegia on the free radical DPPH was determined as 7.63 mg/ml. In the case of the linoleic acid system, the methanolic extract of S. microstegia showed 79.92% inhibition, this value is quite close to the value of synthetic antioxidant reagent, 92.46%.
  • Küçük Resim
    Öğe
    Verbascum pinetorum (Boiss.) O. Kuntze bitki ekstraktının antimikrobiyal ve antioksidan aktivitesinin belirlenmesi
    (Hatay Mustafa Kemal Üniversitesi, 2008) Esen, Mari; Çalışkan, Mahmut
    Scrophulariaceae familyasına ait Hatay endemiği Verbascum pinetorum (Boiss.) O. Kuntze'den elde edilen 5 farklı özütünün antimikrobiyal ve metanolik özütünün antioksidan aktivitesi belirlenmiştir. Artan polarite özütleme ve direkt metanol özütleme yöntemleri sonrasında elde edilen non-polar ve polar özütlerin antimikrobiyal aktiviteleri disk difüzyon yöntemi kullanılarak sekiz adet gram pozitif, altı adet gram negatif bakteri ve bir adet fungus üzerinde test edilmiştir. Direkt metanolik özütün antioksidan aktivite kapasitesi DPPH ve beta-karoten renk açılım yöntemleri kullanılarak çalışılmıştır. Non-polar özütlerden hekzan, diklorometan ve methanol/kloroform özütlerinin, polar methanol/su özütünün ve direkt methanol özütünün toplam olarak üç adet gram pozitif, iki adet gram negatif ve maya suşları üzerinde çeşitli düzeylerde antimikrobiyal etkileri gözlenmiştir. Özüt ve pozitif kontrol olarak kullanılan BHT ve askorbik asitin DPPH radikalinin %50 inhibisyonunu sağlayan IC50 değerleri sırasıyla 14,35 mg/ml, 2,25 mg/ml ve 0,45 mg/ml olarak bulunmuştur. Beta-karoten renk açılımına göre 50. saatin sonunda özütün linoleik asit oksidasyonunu %82, BHT'nin ise %85 oranında inhibe ettiği bulunmuştur. Buna göre V. pinetorum bitkisinden elde edilen özütlerin genel olarak bazı patojen mikroorganizmaların üremesini engellediği ve antioksidan aktivite kapasitesine sahip olduğu belirlenmiştir.