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    Antioxidant and anti-inflammatory activities of Gallic acid in Japanese quails induced by oxidative stress
    (Univ Zulia, Facultad Ciencias Veterinarias, 2023) Isgor, Mehmet Mustafa; Kucukgul, Altug; Alasahan, Sema
    Gallic acid is a phenolic compound found in many plant sources with strong antioxidant activity. In this study, the bioactivity of Gallic acid was investigated in Japanese quails induced by oxidative stress. The study was performed on four groups of 40-day-old male Japanese quail (Coturnix japonica). Oxidative stress was created for 1 week by adding 0.5% hydrogen peroxide. The study was terminated by administering 100 mg center dot kg(-1) body weight Gallic acid intraperitoneally. Total antioxidant and total oxidant level analyzes from liver tissue homogenates were performed using a ready-made commercial kit. TNF-alpha levels from blood samples taken for anti-inflammatory activity were investigated by ELISA method. There were no statistically significant results on live weight gain between the experimental groups and control group. However, Gallic acid in liver homogenates together with H2O2 increased total antioxidant state (TAS) compared to H2O2 application, while it decreased total oxidant state (TOS) in the same groups. Moreover, while the oxidative stress index increased in the H2O2 group, it decreased significantly in both the Gallic acid and Gallic acid + H2O2 groups. Gallic acid application also caused regression in blood TNF-alpha expression levels, which were increased by H2O2. In quails, Gallic acid showed antioxidant activity by increasing TAS levels and decreasing TOS levels, providing a significant decrease in oxidative stress index. It also provided anti-inflammatory activity by suppressing TNF-alpha levels. However, advanced molecular analyzes are needed to obtain more detailed information on the subject.
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    Investigation of the anti-apoptotic activity of ozone therapy in rainbow trout macrophages infected with Yersinia ruckeri
    (Springer, 2019) Kucukgul, Azime; Kucukgul, Altug; Gonenci, Ramazan; Ozsoy, Sule Yurdagul; Kutlu, Banu; Isgor, Mehmet Mustafa
    Yersinia ruckeri is the causal agent of enteric redmouth (ERM) in trout. This study aimed to investigate the effectiveness of nontoxic ozone on Y. ruckeri-infected trout cells in in vitro conditions. Rainbow trout macrophages (RTS-11) were exposed to Y. ruckeri for 24h after being incubated with the ozonized medium at nontoxic concentration. Effective concentrations of nontoxic ozone (3 gamma) and bacteria (1.5x10(8)cfu/mL) were determined by viability tests. The expression of caspases 1, 3, 8, and 12 was determined by real-time PCR and protein levels investigated by ELISA method. Apoptotic-necrotic cell ratios were determined by acridine orange and ethidium bromide staining. Bacterial stimulations of caspases (1, 3, 8, and 12) were suppressed by ozone mostly at the translational level. While bacterial infection increased the count of apoptotic and necrotic cells at different stages, ozone significantly reduced this condition. Obtained data indicate possible use of nontoxic ozone as a safe and effective treatment alternative for increasing host defenses and eliminating the infection in the case of yersiniosis in fish.
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    Role of Epigallocatechin Gallate on In Vitro Model of Methylglyoxal-induced Amyloidogenesis
    (Asian Journal Pharmaceutics, 2016) Kucukgul, Altug; Isgor, Mehmet Mustafa; Cellat, Mustafa; Ata, Hayrettin
    Introduction: The study aimed to investigate the potential effects of epigallocatechin gallate (EG) on the reflux of methylglyoxal (MG)-induced amyloidogenesis in human glioblastoma (U87) cells. Materials and Methods: The effective concentrations of MG and EG were investigated via Trypan blue test. Glyoxalase-1 (GLO-1), beta-amyloid precursor protein (beta APP), and Caspase 3 (Cas 3) expression levels were determined by quantitative real-time polymerase chain reaction and intracellular glutathione (GSH) contents of cells were measured. Results: MG at 250 mu M reduced viable cells by 33.4% as compared to control group. However, 5 mu M EG pre-treatment before MG prevented 22.4% of the cell loss caused by MG (P <= 0.05). MG stimulated beta APP and Caspase 3 levels by 4.13- and 3.46-fold; however, EG pre-treatment inhibited these increases by 1.76 and 3.09, respectively. In addition, EG pre-treatment increased GLO-1 levels by 3.71-fold and GSH levels by 2.30-fold according to MG group. Conclusion: EG demonstrated protective effect against cell death on U87 cells by suppressing amyloidogenic factors and apoptotic stimuli induced by MG.
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    The synthesis of novel pyrazole-3,4-dicarboxamidesbearing 5-amino-1,3,4-thiadiazole-2-sulfonamide moiety with effective inhibitory activity against the isoforms of human cytosolic carbonic anhydrase I and II
    (Academic Press Inc Elsevier Science, 2016) Mert, Samet; Alim, Zuhal; Isgor, Mehmet Mustafa; Beydemir, Sukru; Kasimogullari, Rahmi
    A series of 1-(3-substituted-phenyl)-5-phenyl-N-3,N-4-bis(5-sulfamoyl-1,3,4-thiadiazol-2-yl)-1H-pyrazole-3,4- dicarboxamides (4-15) were synthesized. The structures of these pyrazole-sulfonamides were confirmed by FT-IR, H-1 NMR, C-13 NMR and elemental analysis methods. Human cytosolic carbonic anhydrase (CA, EC 4.2.1.1) isozymes (hCA I and II) were purified from erythrocyte cells by affinity chromatography. The inhibitory effects of newly synthesized derivatives (4-15) were investigated in vitro on esterase activities of these isozymes. The K-i values were determined as 0.119-3.999 mu M for hCA I and 0.084-0.878 mu Mfor hCA II. The results showed that the compound 6 for hCA I and the compound 11 for hCA II had the highest inhibitory effect. Beside that, the compound 8 had the lowest inhibition effect on both isozymes. (C) 2016 Elsevier Inc. All rights reserved.