Role of Epigallocatechin Gallate on In Vitro Model of Methylglyoxal-induced Amyloidogenesis
[ N/A ]
Tarih
2016
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Asian Journal Pharmaceutics
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Introduction: The study aimed to investigate the potential effects of epigallocatechin gallate (EG) on the reflux of methylglyoxal (MG)-induced amyloidogenesis in human glioblastoma (U87) cells. Materials and Methods: The effective concentrations of MG and EG were investigated via Trypan blue test. Glyoxalase-1 (GLO-1), beta-amyloid precursor protein (beta APP), and Caspase 3 (Cas 3) expression levels were determined by quantitative real-time polymerase chain reaction and intracellular glutathione (GSH) contents of cells were measured. Results: MG at 250 mu M reduced viable cells by 33.4% as compared to control group. However, 5 mu M EG pre-treatment before MG prevented 22.4% of the cell loss caused by MG (P <= 0.05). MG stimulated beta APP and Caspase 3 levels by 4.13- and 3.46-fold; however, EG pre-treatment inhibited these increases by 1.76 and 3.09, respectively. In addition, EG pre-treatment increased GLO-1 levels by 3.71-fold and GSH levels by 2.30-fold according to MG group. Conclusion: EG demonstrated protective effect against cell death on U87 cells by suppressing amyloidogenic factors and apoptotic stimuli induced by MG.
Açıklama
Anahtar Kelimeler
Amyloidogenesis, epigallocatechin gallate, in vitro, methylglyoxal
Kaynak
Asian Journal of Pharmaceutics
WoS Q Değeri
N/A
Scopus Q Değeri
Cilt
10
Sayı
3
