Role of Epigallocatechin Gallate on In Vitro Model of Methylglyoxal-induced Amyloidogenesis

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dc.contributor.authorKucukgul, Altug
dc.contributor.authorIsgor, Mehmet Mustafa
dc.contributor.authorCellat, Mustafa
dc.contributor.authorAta, Hayrettin
dc.date.accessioned2024-09-18T19:54:17Z
dc.date.available2024-09-18T19:54:17Z
dc.date.issued2016
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractIntroduction: The study aimed to investigate the potential effects of epigallocatechin gallate (EG) on the reflux of methylglyoxal (MG)-induced amyloidogenesis in human glioblastoma (U87) cells. Materials and Methods: The effective concentrations of MG and EG were investigated via Trypan blue test. Glyoxalase-1 (GLO-1), beta-amyloid precursor protein (beta APP), and Caspase 3 (Cas 3) expression levels were determined by quantitative real-time polymerase chain reaction and intracellular glutathione (GSH) contents of cells were measured. Results: MG at 250 mu M reduced viable cells by 33.4% as compared to control group. However, 5 mu M EG pre-treatment before MG prevented 22.4% of the cell loss caused by MG (P <= 0.05). MG stimulated beta APP and Caspase 3 levels by 4.13- and 3.46-fold; however, EG pre-treatment inhibited these increases by 1.76 and 3.09, respectively. In addition, EG pre-treatment increased GLO-1 levels by 3.71-fold and GSH levels by 2.30-fold according to MG group. Conclusion: EG demonstrated protective effect against cell death on U87 cells by suppressing amyloidogenic factors and apoptotic stimuli induced by MG.en_US
dc.identifier.endpageS420en_US
dc.identifier.issn0973-8398
dc.identifier.issn1998-409X
dc.identifier.issue3en_US
dc.identifier.startpageS414en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12483/7630
dc.identifier.volume10en_US
dc.identifier.wosWOS:000387987100027en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherAsian Journal Pharmaceuticsen_US
dc.relation.ispartofAsian Journal of Pharmaceuticsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAmyloidogenesisen_US
dc.subjectepigallocatechin gallateen_US
dc.subjectin vitroen_US
dc.subjectmethylglyoxalen_US
dc.titleRole of Epigallocatechin Gallate on In Vitro Model of Methylglyoxal-induced Amyloidogenesisen_US
dc.typeArticleen_US

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