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Öğe Readily Accessible and Brightly Fluorogenic BODIPY/NBD-Tetrazines via SNAr Reactions(Amer Chemical Soc, 2024) Isik, Murat; Kisacam, Mehmet AliWe describe SNAr reactions of some commercial amino-tetrazines and halo-dyes, which give efficiently quenched BODIPY/NBD-tetrazines (Phi(Fl) < 0.01) in high yields and, importantly, with high purities affordable via simple silica gel chromatography only. The dyes exhibit large Stokes shifts, moderate environmental sensitivity, and emission enhancements (up to 193-fold) upon Tz ligation with BCN & horbar;a strained dienophile. They successfully serve as labels for HSA protein premodified with BCN, resulting in bright blue-green emission upon ligation.
