Readily Accessible and Brightly Fluorogenic BODIPY/NBD-Tetrazines via SNAr Reactions
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Dosyalar
Tarih
2024
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Amer Chemical Soc
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
We describe SNAr reactions of some commercial amino-tetrazines and halo-dyes, which give efficiently quenched BODIPY/NBD-tetrazines (Phi(Fl) < 0.01) in high yields and, importantly, with high purities affordable via simple silica gel chromatography only. The dyes exhibit large Stokes shifts, moderate environmental sensitivity, and emission enhancements (up to 193-fold) upon Tz ligation with BCN & horbar;a strained dienophile. They successfully serve as labels for HSA protein premodified with BCN, resulting in bright blue-green emission upon ligation.
Açıklama
Anahtar Kelimeler
Bioorthogonal Reactions, Small Molecules, Live Cells, Probes, Cycloaddition, Dyes, Design, 1,2,4,5-Tetrazines, Bicyclononynes, Derivatives
Kaynak
Journal of Organic Chemistry
WoS Q Değeri
N/A
Scopus Q Değeri
Q2
Cilt
89
Sayı
9
