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  • Küçük Resim
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    Apoptotic and Anti-inflammatory Effects of Hypericum Perforatum Extract inHuman Basal Cell Carcinoma TE 354.T Cell Line
    (2021) Celık, Ebru; Kaplan, Halil Mahir; Şingirik, Ergin; Çelik, Muhammed Salih; Alp, Harun
    Objectives: Basal cell carcinoma is the most common malignancy in in the Caucasian race and is a serious health problem with its ever-increasing incidence and high cost. Although the treatment is surgical, some elderly patients do not accept surgical intervention. In this case, non-surgical treatments come into prominence. The Hypericum perforatum (HP) / St. John's Wort plant has been used in the treatment of many diseases for centuries among local people. Here, we aimed to aimed to elucidate the effect and molecular mechanism of the HP plant on BCC cells.Methods: Human BCC cell lines TE 354.T were acquired from ATCC®, then were cultured in Dulbecco's Modified Eagle's Medium. BCC cells were seeded at a concentration of 2x106cells in each flask (n=12). After incubation, while six of the flasks were applied with a 0.8% HP extract and 99.2% medium for 48 hours, no application was made to the control group. The effects of HP on mitochondrial mediated intrinsic and extrinsic apoptotic pathway agents, cell cycle G2 / M checkpoint kinases and inflammatory mediators were investigated in BCC cancer cells. The expression and activities of these mediators in both groups were evaluated by ELISA test. Results: It was determined that HP extract treatment increased the expression of apoptotic proteins (AIF, GADD153, GRP78, caspase-3, Bax) and cell cycle G2 / M checkpoint kinase (Wee1), though it reduced antiapoptotic protein (Bcl-2) and inflammatory mediators (iNOS, COX-2, cPLA2, NFkB) in BCC cell lines.Conclusion: HP extract can support routine chemotherapy on BCC cells with its apoptotic, anti-inflammatory and anti-cancer effect, and may be a beacon of hope in reducing resistance to chemotherapeutic drugs in the treatment of BCC.
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    Investigating of Effects of Metformin on Melanoma Skin Cancer
    (2021) Alp, Harun; Tutun, Hidayet; Altıntaş, Levent; Kaplan, Halil Mahir; Şingirik, Ergin; Kahraman, İbrahim
    Aim: Apoptotic pathways are important for resistance to chemotherapeutics drugs and cancer development. Metformin is a safe, inexpensive and effective drugused to treat type 2 diabetes. Furthermore, although there is evidence of anticancer effects, the mechanisms by which metformin exerts the effects are unclear.Therefore, it was aimed to investigate Bcl-2, kaspaz-3, bax, wee-1, AIF, grp78 and gadd153 levels in metformin treated skin cancer cells. Materials and Methods: The skin cancer cells (B16F10) were treated with metformin and kaspase-3, bcl-2, wee 1, gadd153, grp78 and AIF levels were analyzedby using ELISA. Result: Treatment of metformin increased of proapoptotic proteins (kaspase-3, bcl-2, wee 1, gadd153, grp78 and AIF) and decreased antiapoptotic protein (bcl 2). Conclusion: In conclusion, this study showed that metformin has apoptotic effect on melanoma skin cancer.
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    Investigation of apoptotic effects of hypericum perforatum extract on breast cancer cell line
    (Harran Üniversitesi, 2019) Alp, Harun; Tutun, Hidayet; Kaplan, Halil Mahir; Şıngırık, Ergin; Altıntaş, Levent
    Abstract: Hypericum perforatum has biological active contents affecting a variety of proteins such as caspase-3, bcl-2, and bax, which mediate apoptosis known as programmed cell death and exerting anti-inflammatory effect. Apoptotic pathways are important for cancers, chemotherapeutic resistance, and cancer development. Anti-inflammatory agents are also a potential target for cancer. Therefore, it was aimed to investigate the activity of caspase-3 and the expressions of bcl-2, bax, wee 1, gadd153, grp78, AIF, iNOS, COX-2, cPLA2, and NF-κB in H. perforatum extract-treated breast cancer (BC) cells in this study. The activity of caspase-3 and the expressions of these proteins were determined in the cells by ELISA. The HP extract increased the activity of caspase-3 and the expressions of bax, wee 1, gadd153, grp78 and AIF, and decreased the expressions of bcl-2, COX-2, iNOS, cPLA2 and NF-κB in the BC cells. In the light of these findings, HP extract could help to inhibit grow of BC cells and its anti-inflammatory effect may contribute this effect.
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    Protective Effect of Hypericum perforatum Extract on Methotrexate-Induced Osteotoxicity via Reducing Oxidative Stress and MAPK Activity
    (Asian Network Scientific Information-Ansinet, 2020) Uruc, Vedat; Salimov, Fariz; Kaplan, Halil Mahir
    Backgrounds and Objective: Methotrexate (MTX) is an anti-metabolite and anti-rheumatic drug that is widely used in cancer treatment. It is known that a high dose of MTX chemotherapy causes bone growth disturbance in developing bones. Hypericum perforatum (HP) plant is useful against some diseases for local people and regulates apoptosis which is also known as programmed cell death and recent studies also reported that HP reduces the side effects of many toxic drugs. For this reason, we planned a study to reveal the osteoprotective effect of HP extract against MTX induced osteo-toxicity. Materials and Methods: For this purpose MLO-Y4 cells were cultured, then these cells were treated with HP and methotrexate and mediators of the apoptotic, MAPK pathway and the oxidative stress parameters were analyzed on these cells. Results: Expression of proapoptotic factors increased and expression of antiapoptotic factors were decreased with MTX treatment. Treatment of HP ameliorated the apoptotic effect of MTX on MLO-Y4 cells. HP treatment also inhibited MAPK activity and oxidative stress induced by methotrexate. Conclusion: HP attenuated methotrexate-ind uced osteo-toxicity via reducing oxidative stress and MAPK activity and HP is an effective reducing side effect of MTX.