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Öğe APPLICATION OF NEAR INFRARED SPECTROSCOPY COMBINED WITH MULTIVARIATE ANALYSIS FOR SCREENING FOLIAR MAIN ESSENTIAL OIL COMPONENTS IN BAY LAUREL(Soc Brasil Engenharia Agricola, 2022) Celiktas, Nafiz; Kaya, Alpaslan; Turkmen, MusaGround bay laurel leaf samples (10-15 g) were scanned using Fourier-transform nearinfrared (FT-NIR) spectrometer with reflectance mode in the 1000-2500 nm wavelength range. According to the wet chemical analyses, the essential oil content of the samples from different locations varied between 1.77 and 5.30%. The major component of essential oil was 1-8 cineole with a concentration of 43.4-58.1%. The regression coefficients of calibration (R-C(AL))2 and validation (R-VAL(2)) for essential oil and 1-8 cineol content with partial least square regression (PLSR) actualized as 0.96-0.98 and 0.98-0.98, respectively. The prediction accuracy of the final NIRS model was reasonable, with acceptable root mean standard errors of prediction (RMSEP) of 0.18% and 0.45%. According to the residual predictive deviation (RPD) index (3.58 and 8.41), the accuracy of the NIRS models was regarded as the best. The PLSR model differentiated bay laurel genotypes very well on the first principal component (PC1), based on the related properties.Öğe Hatay florasında yetişen karabaş lavantanın (Lavandula stoechas subsp. stoechas L.) çelikle köklendirilmesi üzerine farklı lokasyonların ve hormon dozlarının etkisi(2000) Ayanoğlu, Filiz; Mert, Ahmet; Kaya, AlpaslanThe intensive growing areas of Karabaş lavender were first determined in the province of Hatay. The rooting capabilities of the cuttings of Karabaş Lavender which were gathered from different locations, one (Işİklİ) on the coast of Mediterranean and the other one (Narlİca) near Antakya, were investigated. The cuttings were treated with 1 000, 2 000 and 4 000 ppm IBA doses and the cuttings with no IBA treatment were used as control. The effects of IBA on rooting were investigated. The rooting ratios of the cuttings gathered from Işİklİ were higher than those of the cuttings gathered from Narlİca. The hormone doses positively affected the rooting of cuttings gathered from both places, and the rooting ratios, the length of roots and the number of roots per cutting increased with the hormone doses. The highest rooting ratio (70%) was obtained from the cuttings gathered from Işİklİ, treated with 4000 ppm IBA dose.Öğe Hepatoprotective Activity of Linalool in Rats Against Liver Injury Induced by Carbon Tetrachloride(Verlag Hans Huber, 2020) Altinok-Yipel, Fulya; Tekeli, Ibrahim Ozan; Ozsoy, Sule Yurdagul; Guvenc, Mehmet; Kaya, Alpaslan; Yipel, MustafaThis study aimed to investigate and compare hepatoprotective activity of Coriandrum sativum (Cs) and it is major component linalool (Ln) against experimentally induced hepatotoxicity in rats. Essential oil of Cs was isolated by hydrodistillation method and chemical composition was determined by GS-MS analysis. 42 male Wistar Albino rats were divited into 7 groups each containing 6. The experimental groups were designed as: Normal control group, 1 ml/kg CCl4 administirated group, 25 mg/kg Silymarin and CCl4 administirated group, 100 and 200 mg/kg Cs and CCl4 administirated groups, 100 and 200 mg/kg Ln and CCl4 administered groups. The protective activities were determined according to the results of liver biomarkers (AST, ALT, ALP), antioxidant parameters (GSH, GPx, CAT), lipid peroxidation (MDA) and histopathological examination. Linalool percentage of Cs was 81.6%. The groups treated with linalool (100 and 200 mg/kg) (p < 0.01) and coriander (200 mg/kg) (p < 0.05) had significantly reduced AST (262-375) and ALT (101-290) levels (U/L) compared to the CCl4 (600-622) group. The levels (nmol/g protein) of MDA (11-12) were significantly lower (p < 0.01), the levels of GSH (11-12) and the activities of CAT (23-24) were significantly higher (p < 0.01) in linalool groups (100 and 200 mg/kg) compared to the CCl4 (18-5-10 respectively) group. These results were also supported by histopathological findings and indicate that Cs and Ln shows hepatoprotective activity against liver damage. In this regard, evaluation of activities of major components are needed to compare to medicinal plants in experimental diseases models.Öğe In vitro antiviral effect of the essential oils of thymbra spicata L. on herpes simplex virus type 2(2012) Duran, Nizami; Kaya, Alpaslan; Duran, Gulay Gulbol; Eryilmaz, NaciyeThe essential oils of Thymbra spicata L. has been used for a long time as a folk medicine to treat a lot of diseases. In this study, we aimed to investigate the antiviral effect of The essential oils of Thymbra spicata L. against HSV-2 (Herpes Simplex Virus type 2). Aerial parts of Thymbra spicata L. were used. They harvested at full flowering stage in July 2011 from the botanical gardens, Field Crops Department of Mustafa Kemal University. Chemical analysis of Thymbra spicata L. was performed by GC-MS. In the study, HEp-2 cell line derived from human larynx cancer cells was used. Cultivation of HEp-2 cells was carried out in RPMI 1640 medium with 10% fetal calf serum at an atmosphere of 37°C with 5% CO2. The cell viability was evaluated by a tetrazolium-based colorimetric method using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). The main components with the highest rates were carvacrol, cymol, gamaterpinene and thymol. While, typical cytopathological changes for HSV-2 on the cells was observed at low Thymbra spicata L., concentrations (10 and 20 ?g/ml), no cytopathological changes was observed at high Thymbra spicata L., concentrations (40, 80 and 160 ?g/ml). In these concentration, HSV-2 could not affect HEp-2 cells. The present results indicated that Thymbra spicata L., with a minimum concentration value of 40 ?g/ml significantly showed antiviral effect against HSV-2 compared to the control group (p<0.001). This study shows that the essential oils of Thymbra spicata L. seems to have notable antiviral activity for HSV-2. We think that this antiviral effect of the essential oils of Thymbra spicata L may be attributed the abundant components in the structure such as Carvacrol, cymol, gama-terpinene and thymol. Our results confirmed that the essential oils of Thymbra spicata L. is a potential source of new and selective agent for the treatment of HSV-2 infection. However, further studies are needed to understand its antiviral mechanism and before making a recommendation.Öğe Propagation of some native grown medicinal plants by stem cuttings(2002) Ayanoglu, Filiz; Mert, Ahmet; Erdogan, Cahit; Kaya, AlpaslanSalvia indica L., Helichrysum plicatum subsp, plicatum D.C., and Satureja thymbra L, are some native grown plants which are used as folk medicine in Hatay province, Turkey. The propagation possibilities of these plants by stem cuttings were investigated, Different IBA (Indole 3-butiric acid) doses were applied to 10-12 cm cuttings as a rooting agent: 100, 250, 500, 1000 and 2000 ppm for H. plicatum; 250, 500, 1000, 2000 and 4000 ppm for S. thymbra; and 100, 250, 500, 1000, 2000 and 4000 ppm for S. indica L. The experimental design was a completely randomized block with four replications. Each replication had 25 cuttings. Cuttings were immersed in different IBA doses for 5 seconds. No IBA was applied to the control cuttings. Rooting ratio (%), root length (cm), root number (number/cutting) and rooting quality of cuttings were measured. The best rooting ratios were obtained from 250 and 1000 ppm IBA doses for H. plicatum, 1000 and 2000 ppm for S. thymbra and S. indica. © 2002 by The Haworth Press, Inc. All rights reserved.
