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Öğe The architecture of the lymph nodes in the abdominal and thoracic cavities of wild boar(Agricultural Research Communication Centre, 2019) Bozkurt, Yesim Akaydin; Ates, Sevinc; Kozlu, Tolunay; Basak, FeyzaThe distrubition of lymph nodes located in the abdominal and thoracic cavities of ten wild boars, and their structure were determined anatomically, histologically and immunohistochemically, to be the first detailed investigation on the wild boar. Though general localization and distribution were similar, the number of lymph nodes showed small differences from those of domestic pig. Histological investigations did not reveal a significant hilus. Besides, T lymphocytes with anti-CD3, CD4, CD8, B lymphocytes with anti-CD79a, macrophages with anti-macrophage monoclonal antibodies, and follicular dendritic cells using anti-S100 polyclonal antibody and their distrubution in the lymph nodes were detected. Many CD3 positive T lymphocytes were observed in the germinal center of the lymph follicles, in the cortical area and in the medulla. CD8 positive T lymphocytes were few, and CD4 positive T lymphocytes were not seen. CD79 positive cells were scanty.Öğe A COMPARATIVE STUDY ON THE HISTOLOGICAL STRUCTURE OF THE SPLEEN IN THE OSTRICH (STRUTHIO CAMELUS), THE KESTREL (FALCO TINNUNCULUS) AND THE OSPREY (PANDION HALIAETUS)(Akademiai Kiado Rt, 2011) Kozlu, Tolunay; Sari, Ebru Karadag; Bozkurt, Yesim Akaydin; Altunay, H.The spleen structurally and functionally belongs to the hematopoietic organs and is also an important component of the reticuloendothelial system, which is known to play a major role in host defense. The histological structure of the spleen was investigated in the ostrich, a non-flying bird, the kestrel, a raptor, and the osprey, a fish-eating bird of prey (fish eagle). For this purpose, Mallory's modified triple stain, methyl green-pyronin and silver stain were used. Germinal centers were not present in the spleen of the osprey. In the spleen of the kestrel, penicillar arterioles and the surrounding lymphoid tissue were markedly dense. Compared to the other two birds, the red and white pulps were clearly distinguishable in the spleen of the ostrich.Öğe Does Diabetes Affect the Carbohydrate Secretions of the Endometrial Glands of Rats in Early Pregnancy?(2020) Başak, Feyza; Kozlu, TolunayNourishment for the embryo. Diabetes is known to alter the secretions of certain cells. This study aimed to identify thecarbohydrate content of endometrial gland secretions using Peanut Aglutinin (PNA), Wheat Germ Agglutinin (WGA)Concanavalin-A (Con-A), Soy Bean Agglutinin (SBA).Materials and Methods: Thirty-two Wistar Albino rats were divided into 4 groups of 8; diabetes and pregnancy positive,diabetes positive, pregnancy positive, control group. Samples were collected on the 5th and 7th day of pregnancy, and in thevaginally stimulated non-pregnant rats.Results: Positive PNA staining occurred in both groups with diabetes and was negative in the other two groups. Diabetesproduced marked alterations in SBA staining in the early days of pregnancy, but did not persist for long. With regard toWGA, when diabetes combined with pregnancy, changes in the secretion of the endometrial glands occur. Con-A stainingshowed that both pregnancy and diabetes affect the amounts of the ?-mannose chain, ?-chain glucose, and N-acetylglucosamine ?-chain components of endometrial gland secretions.Conclusion: In conclusion this study shows that both diabetes and pregnancy affect the carbohydrate content of thesecretions from the endometrial glands.Öğe The effect of Theranekron® in intact and ischemia-reperfusion injured rat ovary(2023) Güler, Fatma; Kozlu, Tolunay; Ergün, Yaşar; Alan, Beyza Suvarıklı; Tutar, TolgaThe effect of Theranekron® on ovaries were evaluated in healthy and ischemia- reperfusion injury rat model. Thirty-eight female, nulligravida Wistar Albino rats were divided into four groups, as follows: group 1: control, group 2: Theranekron®, group 3: ischemia-reperfusion and group 4: ischemia-reperfusion+Theranekron®. Each groups were analyzed biochemically and histologically. As compared with group 1, there was a significant increase in the number of atretic follicles and interstitial cells in group 2. Also, nitrite, nitrate, and malondialdehyde levels increased significantly in group 2. Group 3 represented intense hyperemia and hemorrhages on follicles surrounding the cortex and in the corpus luteum and marked increase in the number of atretic follicles, compared to group 2. Nitrite and nitrate levels in group 3 were similar to those in group 2, but malondialdehyde levels were lower than those in group 2. Group 4 showed very slight decrease in interstitial cells compared with group 2 and group 3. In this group, malondialdehyde levels decreased compared to group 2 and increased compared to control and group 3. Based on the results obtained, we may speculate that Theranekron® may have some degenerative effects on intact ovaries, and may have ameliorating effects on ischemia reperfusion injury in ovaries.Öğe Effects of Erdosteine on Acetaminophen-induced Hepatotoxicity in Rats(Sage Publications Inc, 2008) Kuvandik, Guven; Duru, Mehmet; Nacar, Ahmet; Yonden, Zafer; Helvaci, Rami; Koc, Ahmet; Kozlu, TolunayWe investigated the effects of erdosteine on acetaminophen (APAP)-induced hepatotoxicity in rats. Superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), AST (aspartate aminotransferase), and ALT (alanine transaminase) activities, and malonyldialdehyde (MDA) and nitric oxide levels as oxidant/antioxidant biochemical parameters were investigated with light microscopic evaluation in adult female Wistar Albino rats. APAP administration produced a decrease in hepatic SOD, CAT, and GSH-Px activities, and coadministration of erdosteine (150 and 300 mg/kg) resulted in increases in the activities. MDA and NO levels increased in the APAP group, and erdosteine treatments prevented these increases. Significant elevations in serum AST and ALT levels were observed in the APAP group, and when erdosteine and APAP were coadministered, their serum levels were close to those in the control group. Light microscopic evaluation of livers showed that there were remarkable centrilobular (zone III) hepatic necrosis and mild to moderate sinusoidal congestion in the APAP group, whereas in the erdosteine group, cellular necrosis was minimal and the hepatocytes maintained a better morphology when compared to the APAP group. Erdosteine prevented APAP-induced liver injury and toxic side effects probably through the antioxidant and radical scavenging effects of erdosteine.Öğe Harder bezi’nin yapı ve fonksiyonları(2010) Kozlu, Tolunay; Altunay, HikmetHarder bezi, karada yaşayan omurgalıların pek çoğunda bulunan orbital bir bezdir. Harder bezi ilk defa Johann Jacop Harder tarafından 1694 yılında iki geyik türünde yaptığı bir araştırma ile keşfedilmiştir. Memeli Harder bezi üçüncü göz kapağı ile ilişkili olarak gözün iç açısı içinde yer alır ve sekretorik kanalı ile membrana niktitans’ın yüzeyine açılır. Kanatlılarda bileşik tubuler veya tubuloalveolar tipteki Harder bezi’nin lumenini değişen yükseklikte prizmatik hücrelerin oluşturduğu epitel sınırlandırır. Bezin subepitelyal bölgesinde plazma hücreleri bulunur. Harder bezi sürüngenlerde genellikle göz küresinin mediyaline ya da mediyoventraline yerleşir. Bu bez yılanlarda bilinen tek göz bezidir. Türlere göre Harder bezi’nin morfolojik ve biyokimyasal özellikleri arasındaki farklılık şaşırtıcıdır ve bu farklılığın, adaptasyon ihtiyaçlarını karşılayacak şekilde değişen fonksiyonlar nedeniyle oluştuğu düşünülmüştür. Ayrıca son yıllarda bazı araştırmacılar, primatlarda da bezin varlığını ortaya koymuşlardır. Bu derlemenin amacı hayvan türlerine göre değişen ve önemli fonksiyonlara sahip Harder bezi’nin yapısal ve fonksiyonel olarak değerlendirilmesidir.Öğe Histological and Histochemical Studies on the Harderian Gland of the Osprey (Pandion haliaetus)(Medwell Online, 2010) Kozlu, Tolunay; Bozkurt, Yesim Akaydin; Altunay, Hikmet; Sari, Ebru KaradagThe aim of the present study was to demonstrate the histological and histochemical structure of the Harderian gland of the osprey. The Harderian gland of the osprey (Pandion haliaetus) is a tubuloalveolar gland. The gland lumen was lined by columnar epithelial cells of varying height which were filled with secretory vesicles and by dark cells found among these cells. It was observed that the secretion was produced in the corpus glandulae and was extracted by primary and secondary ducts. In addition, plasma cells were present in interlobular trabeculae. Histochemical stainings which were performed to determine the character of the glandular secretion revealed that the majority of the secretory cells contained only acidic mucins whilst the remaining secretory cells contained both neutral and acidic mucins. Furthermore, it was observed that some cells of the primary and secondary ducts epithelium had secretory vesicles containing only neutral mucin. The presence of reticulum fibres was demonstrated in the interlobular trabeculae.Öğe Immunohistochemical staining of cd3, cd79?cy and s-100 on bursa fabricius, thymus and spleen of turkeys (Meleagris gallapavo)(Agricultural Research Communication Centre, 2019) Kozlu, Tolunay; Sari, Ebru Karadag; Bozkurt, Yesim Akaydin; Kurtdede, NevinDistribution of CD3, CD79 alpha cy and S-100, immunohistochemically, in the bursa of Fabricius, thymus and spleen has not been reported in turkeys. Therefore, we determined the localisation of anti-CD3 protein antibodies for mature T-lymphocytes, anti-CD79 alpha cy antibodies for B-lymphocytes and anti-S-100 protein antibodies for follicular dentritic cells in the bursa of Fabricius, thymus and spleen, which are the lymphoid organs, in a study sample of turkeys. Triple staining method was applied to demonstrate the general structure. Moreover, in all the organs positive reactions were observed with the CD3, CD79 alpha cy and S-100 antibodies. It was also found that similar areas had a positive reaction with CD79 alpha cy and S-100 in all of the tested organs. It was remarkable that CD79 alpha cy reacted positively on Hassall's corpuscles (strongly) and the reticular cells (weakly) in the medulla of the thymus instead of the B-lymphocyte positive areas.Öğe The influence of Theranekron® on glucose-6-phosphate dehydrogenase activity in rat ovaries, intact and with ischemia-reperfusion injury(Univ Zagreb Vet Faculty, 2021) Kozlu, Tolunay; Guler, Fatma; Akalin, Pinar Peker; Kazak, Filiz; Ergun, Yasar; Aslan, ErdoganThe effect of Theranekron (R) on rat ovaries was evaluated in healthy and ischemia-reperfusion injury models. The rats were divided into four groups: group 1: control, group 2: Theranekron (R) (single dose of 0.3 mg/kg intraperitoneally), group 3: torsion + detorsion, and group 4: torsion + detorsion + Theranekron (R) (single dose 0,3 mg/kg. intraperitoneally). The ovaries were homogenized by sonication before the analysis and supernatant glucose-6-phosphate dehydrogenase activity, reduced nicotinamide adenine dinucleotide phosphate, reduced glutathione and total protein levels were evaluated spectrophotometrically. Ovary tissues were histologically examined. In group 2, glucose-6-phosphate dehydrogenase activity was significantly reduced (P<0.01) compared to the control group and an increased number of atretic follicles, and hyperaemic and haemorrhagic regions were seen histologically: in group 3. although glucose-6-phosphate dehydrogenase activity was not changed, intense hyperaemia and hemorrhage was observed in the veins of the medulla region and in the cortex. Group 4 showed a decrease in glucose-6-phosphate dehydrogenase activity compared to the control group (P<0.01). Histologically, when compared to group 3, group 4 showed fewer atretic follicles and decreased hyperaemia and hemorrhage in the ovaries, excluding the medulla region. There were no significant differences regarding reduced glutathione and reduced nicotinamide adenine dinucleotide phosphate levels between the groups. The Theranekron (R) dose applied had some negative effects, such as reduced glucose-6-phosphate dehydrogenase activity. an increased number of atretic follicles, and hyperemia in the corpus luteum and medulla region in intact rat ovaries. Although it reduced glucose-6-phosphate dehydrogenase activity, Theranekron (R) may have had slight remedial effects on rat ovaries with ischemia-reperfusion injury.Öğe Light and Electron Microscopic Studies of the Quail (Coturnix coturnix) Harderian Gland(Medwell Online, 2011) Kozlu, Tolunay; Altunay, HikmetThe aim of this study was to present the light and electron microscopic structure of the Coturnix coturnix harderian gland. The quail harderian gland was a single lobe of glandular tubuloalveolar profiles with columnar epithelial cells of varying heights. The secretory cells contained abundant mitochondria and well-developed rough endoplasmic reticulum and varying numbers of secretory vesicles in their cytoplasm (type I). These cells exhibited apically located microvilli. The secretion was collected by the ducts of harderian gland which were generally lined by a single layer of epithelium varying from cuboidal to columnar. The mode of secretion of the quail harderian gland was of holocrine and the secretory cells reacted positively with PAS. Myoepithelial cells were present in the basal region of secretory cells. The plasma cells were observed in the subepithelial region of the gland both among the corpus glandulae and in the connective tissue between the ducts. No difference was observed between sexes.Öğe Light and scanning electron microscopic studies on the lingual papillae of 80 day old wild pig fetal siblings(2013) Ateş, Sevinç; Bozkurt Akaydın, Yeşim; Kozlu, Tolunay; Alan, Aydın; Düzler, AyhanThis study examined the lingual papillae of 80-day-old wild pig fetal siblings through macroscopic and light and scanning electron microscopic observations. Te results revealed that fungiform papillae were located on every aspect of the tongue except the ventral surface and the center of the dorsal surface. Conical papillae were present on the dorsal surface of the root of the tongue and marginal papillae were observed on the lateral edges of the tip of the tongue. Tere were 2 vallate papillae on the dorsal and 2 foliate papillae on the lateral aspects of the root of the tongue. Te fliform papillae normally seen in the mature stage were not observed at this stage. Taste buds were identifed in all the gustatory papillae examined. On the other hand, taste pores were perceived only in the fungiform papillae. Te fndings acquired will surely contribute to the literature as an essential base for further related research.Öğe Light and scanning electron microscopic studies on the lingual papillae of 80-day-old wild pig fetal siblings(Tubitak Scientific & Technological Research Council Turkey, 2013) Ates, Sevinc; Bozkurt, Yesim Akaydin; Kozlu, Tolunay; Alan, Aydin; Duzler, AyhanThis study examined the lingual papillae of 80-day-old wild pig fetal siblings through macroscopic and light and scanning electron microscopic observations. The results revealed that fungiform papillae were located on every aspect of the tongue except the ventral surface and the center of the dorsal surface. Conical papillae were present on the dorsal surface of the root of the tongue and marginal papillae were observed on the lateral edges of the tip of the tongue. There were 2 vallate papillae on the dorsal and 2 foliate papillae on the lateral aspects of the root of the tongue. The filiform papillae normally seen in the mature stage were not observed at this stage. Taste buds were identified in all the gustatory papillae examined. On the other hand, taste pores were perceived only in the fungiform papillae. The findings acquired will surely contribute to the literature as an essential base for further related research.Öğe A Macroanatomical and Histological Study of the Uropygial Gland in the White Stork (Ciconia cicionia)(Soc Chilena Anatomia, 2011) Kozlu, Tolunay; Bozkurt, Yesim Akaydin; Ates, SevincThe present study was aimed at the macroanatomical and histological investigation and the demonstration of the structural characteristics of the uropygial gland in the white stork. The uropygial gland of two adult male white storks constituted the material of the study. It was determined that the gland was situated subcutaneously in between the caudal aspect of the lumbosacral bone and the first coccygeal vertebrae. The gland was composed of two lobes, which displayed an oval appearance when viewed from above and resembled a water drop when observed medially. Tuft of feathers were present on the caudal margin of each lobe. The present study revealed that the uropygial gland of the white stork is a simple tubular gland, which discharges its secretion into the lumen by a holocrine mode. Each lobe, possessed centrally located large cavities and a main excretory duct and displayed the presence of externally situated peripheral tubules and interiorly situated central tubules. These secretory tubules, which composed the parenchyma, were separated by connective tissue septa referred to as trabeculae, and released their secretion into a centrally located lumen. The wall of the secretory tubules was comprised of four layers, referred to as the germinative, intermediary, secretory and degenerative layers. The silver-staining procedure demonstrated that the stroma of the uropygial gland did not contain reticulum fibres. The anatomy and histology of the gland has been examined in few avian species. Up to date, this study is the first to describe the histological and anatomical peculiarities of the uropygial gland in white stork. The results acquired hereby will surely contribute to further studies to be conducted on the related area.Öğe The protective effect of erythropoietin and dimethylsulfoxide on ischemia-reperfusion injury in rat ovary(Elsevier Science Bv, 2010) Ergun, Yasar; Koc, Ahmet; Dolapcioglu, Kenan; Akaydin, Yesim; Dogruer, Gokhan; Kontas, Tunay; Kozlu, TolunayObjectives: The aim of this study was to investigate the effects of erythropoietin and dimethylsulfoxide in the recovery from ischemia-reperfusion injury in an experimental rat adnexal torsion model. Study design: Thirty-six Wistar-albino rats were divided into six groups. Except for the sham operation group, all groups were subjected to left unilateral adnexal torsion for 3 h. Erythropoietin and dimethylsulfoxide were intraperitoneally administered 30 min before the detorsion operation. Malondialdehyde and nitric oxide levels were detected from both the plasma and the tissue samples. The sections of the tissues were evaluated histologically. The results were analyzed by a one-way analysis of the variance (ANOVA) followed by the Duncan test for multiple comparisons using computer software, SPSS Version 15.0 for Windows. Results: This study demonstrated that dimethylsulfoxide and erythropoietin pretreatment attenuated ischemia-reperfusion-induced lipid peroxidation, prevented post-ischemic ovarian injury and helped to maintain the ovarian morphology. Malondialdehyde levels of plasma and ovary were higher in the torsion and detorsion groups than the sham group. This showed that ischemia-reperfusion had caused lipid peroxidation of the ovarian tissue, thus leading to oxidative damage. One of the major findings of this study is that malondialdehyde was significantly decreased in the plasma of rats who were pre-treated with dimethylsulfoxide and erythropoietin before detorsion. This suggests that dimethylsulfoxide and erythropoietin might prevent oxidative damage in ovarian ischemia-reperfusion injury. Histological examination confirmed that reperfusion caused more detrimental effects than only ischemia, which could be at least partially prevented by dimethylsulfoxide and erythropoietin administration prior to detorsion. Conclusion: Erythropoietin and dimethylsulfoxide may have beneficial effects in ischemia-reperfusion injury in ovarian torsion. (C) 2010 Elsevier Ireland Ltd. All rights reserved.Öğe Ratlarda prenatal, postnatal ve erişkin dönemlerde glandula submandibularis’in gelişimi üzerinde ışık mikroskobik çalışmalar(2010) Yıldız, Hülya; Akaydın Bozkurt, Yeşim; Kozlu, Tolunay; Koç, AhmetBu araştırmada, prenatal, postnatal ve erişkin dönemlerdeki ratların glandula submandibularislerinin histokimyasal açıdan karşılaştırmalı olarak incelenmesi amaçlandı. Çalışmada materyal olarak Wistar albino türü 7 adet erişkin rat ile 7 adet fetüs ve 7 adet yavrudan alınan doku örnekleri kullanıldı. Prenatal dönemde, korpus glandulelerin yavru ve erişkin dönemdekilere oranla daha az sayıda bulundukları ve intersitisyumun postnatal ile erişkin ratlara kıyasla daha geniş alan oluşturduğu belirlendi. Postnatal ve erişkin dönemlerde korpus glandule sayısının ise oldukça artmış olduğu gözlendi. Postnatal ve erişkin dönemlerde akıtıcı kanalların prenatal döneme kıyasla daha gelişmiş oldukları görüldü. Erişkin ratlarda, prenatal ve postnatal ratlarda görülmeyen 3 farklı hücre türünden oluşan granüler kanallara rastlandı. Bu kanallarda hücrelerin içerdikleri granüllerin zayıf PAS pozitif reaksiyon verdikleri saptandı. Histokimyasal metotlar, her üç dönemde de korpus glandule’leri oluşturan salgı epiteli hücrelerinin zayıf PAS pozitif, AB negatif reaksiyon verdiklerini, bazı korpus glandule’lerin ise PAS, AB ve PAS/AB boyamalarında pozitif boyandıklarını gösterdi.Öğe Seksen günlük yaban domuzu fetuslarında kalbin intraventriküler yapılarının morfolojisi(2017) Takcı, Lütfi; Ateş, Sevinç; Başak, Feyza; Akaydın Bozkurt, Yeşim; Kozlu, Tolunayİnsanda ve birçok hayvan türünde kalbin makro ve mikro yapısının ortaya konulduğu çeşitli çalışmalar bulunmaktadır. Ancak yaban domuzu fetuslarında ayrıntılı morfolojik bir çalışmaya rastlanılmamıştır. Dolayısıyla bu çalışmanın var olan bilgi eksikliğinin giderilmesine katkı sağlayacağı düşünülmektedir. Çalışmada %10'luk formaldehit ile tespit edilen 80 günlük yaban domuzu fetusuna ait 7 adet kalp kullanıldı. Diseksiyon ve fotoğraflama işlemlerinden sonra alınan doku örneklerine rutin histolojik prosedür uygulandı. Hazırlanan bloklardan elde edilen 5 mikronluk kesitlere genel histolojik yapıyı ortaya koymak amacıyla Crossman'ın modifiye triple boyama tekniği uygulandı. Valva atrioventricularis dextra'nın incelenen tüm kalplerde cuspis septalis, cuspis angularis ve cuspis parietalis olmak üzere üç yapraktan oluştuğu belirlendi. Valva atrioventricularis sinistra'nın incelenen tüm kalplerde cuspis septalis ve cuspis parietalis olmak üzere iki yapraktan oluştuğu belirlendi. Ventriculus dexter'de dış duvara yerleşmiş 1 adet m. papillaris magnus'a, septal duvara yerleşmiş 1 adet m. papillaris subarteriosus'a ve 1-2 adet mm. papillares parvi'ye rastlandı. Ventriculus sinister'in dış duvarına yerleşmiş 1 adet m. papillaris subauricularis ve 1 adet m. papillaris subatrialis'e rastlandı. Sağ ventriküllerin hepsinde trabeculae septomarginalis'e rastlanırken, sol ventrikülde rastlanmamıştır. Sağ ventrikülde chordae tendinea sayısının 17-24 adet, sol ventrikülde ise 15-20 adet arasında değiştiği belirlenmiştir. Kalbin atrioventriküler hattı boyunca alınan transversal kesitlerde sol ventrikülün dış duvarında subendokardial purkinje hücreleri görüldü. Kalbin uzun ekseni boyunca gömülmesiyle elde edilen kesitlerde ise sol ventrikül septal duvarının subendokardında atrioventricular bundle ve Purkinje hücre toplulukları görüldü. Ayrıca sağ ventrikülün dış duvarından alınan kesitlerde periarterial purkinje hücrelerine rastlandı. Yapılan inceleme sonucunda 80 günlük yaban domuzu fetuslarına ait kalplerde bütün yapıların oluştuğu tespit edilmiştirÖğe Subacute toxicity of piperonyl butoxide and resmethrin in mice(Academic Journals, 2012) Urek, Selma Yazar; Baydan, Emine; Yurdakok, Begum; Akaydin, Yesim; Kozlu, Tolunay; Tunca, AyseThe subacute toxic effects of separate and combined use of piperonyl butoxide and resmethrin (cismethrin) on the liver and kidneys of male mice were investigated. It is known that when given alone, pyrethroids are toxic and their toxicity becomes more complicated when they are co-formulated with piperonyl butoxide. In the present study, macroscopic and microscopic changes were determined in the liver and kidney tissues. Furthermore, biochemical alterations and clinical neurotoxic effects were observed. Toxic effects were more evident in the group subjected to combined use. The results obtained demonstrated that, in mice, piperonyl butoxide and resmethrin are directly toxic to the liver and kidneys. The toxic effects and tissue degeneration were more widespread in the group subjected to combined use.Öğe Sıçanlarda prenatal, postnatal ve erişkin dönemlerde glandula parotis’in gelişimi üzerinde histokimyasal ve ışık mikroskobik çalışmalar(2010) Altunlu, Elif; Koç, Ahmet; Kozlu, Tolunay; Akaydın Bozkurt, Yeşim; Ateş, SevinçBu çalışmada, prenatal, postnatal ve erişkin dönemlerdeki ratların glandula parotis’lerinin histokimyasal ve rutin histolojik boyama yöntemleri kullanılarak gelişimlerinin karşılaştırmalı olarak incelenmesi amaçlandı. Araştırmada prenatal dönem için intra uterin 17 günlük, postnatal dönem için 30 günlük yavru ile yaklaşık 6 aylık erişkin ratlardan parotis bezleri materyal olarak kullanıldı. Prenatal dönemde, stromanın (bağ doku) çok geniş yer kapladığı; bezin salgı yapıcı kısımları ve akıtıcı kanalların ise bezin bağ dokusu içinde küçük kümeler halinde dağılmış olduğu tespit edildi. Öncül salgı hücrelerinin prenatal dönemde, yavru ve erişkin dönemde bulunan hayvanlardaki korpus glandule miktarına oranla çok daha az sayıda olduğu bulundu. Periodik Asit-Schiff (PAS) boyama yönteminde öncül salgı yapıcı hücre topluluklarının bir kısmının PAS pozitif reaksiyon verdiği izlendi. Postnatal dönemde, korpus glanduleyi oluşturan hücreler PAS negatif olarak gözlemlendi. Pars sekrotorya’da PAS ile reaksiyon görülmemesine rağmen pars inisyalis hücre sitoplazmalarında PAS ile reaksiyon tespit edildi. Erişkin dönemde korpus glandule’lerin PAS ile zayıf reaksiyon verdikleri gözlendi. Histokimyasal olarak Alcian Blue (AB) yöntemi ile prenatal dönemde bazı korpus glandule hücrelerinin pozitif reaksiyon vermelerine rağmen, diğer iki dönemde AB negatif reaksiyon verdikleri belirlenmiştir. Her üç dönemde de bağ dokuda çok miktarda mast hücresine rastlandı. Plazma hücreleri postnatal ve erişkin dönemlerde, özellikle pars sekretoryaların çevresinde gözlendi.
