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Öğe Changes in germination capacity of Foeniculum vulgare Mill. Taxa(2002) Ayanoğlu, Filiz; Mert, Ahmet; Aslan, Neşet; Gürbüz, BilalProtection of propagation material of several medicinal plants in the frame of a genebank project is being carried out in our department. In the present work we have examined the changes in germination capacity of Foeniculum vulgare Mill. (Apiaceae) of different origins. The four years' results revealed different reactions of the tested seeds in respect of germination capacity. The effects of storage temperature, storage duration, ripening phase, intraspecific taxa and vegetation year were examined in detail. © 2002 by The Haworth Press, Inc. All rights reserved.Öğe Effects of different drying methods on components of Thymbra spicata L. essential oil from flora of Hatay (Turkey)(Certex, INCDTP-ICPI, 2016) Mert, Ahmet; Türkmen, Musa; Bahadirli, Nadire Pelin; Kaya, Durmus Alpaslan; Ayanog?lu, Filiz; özturk, S¸evketZahter (Thymbra spicata), a perennial member of the Labiatae family is one of the most important spices in Hatay's cuisine. Its dry leaves are mixed with red pepper, sesame and ço¨kelek (a special cheese in the region) and is heavily used in su¨rk (a dish made with ço¨kelek) and pastries. This study aims to determine the effects of various drying methods on the essential oil content of thymbra spicata, a naturally growing plat in Hatay. The plant material was harvested from Hatay's flora on May 4, 2015 and dried in the shade, in an oven at 30°C. The essential oil was obtained with vapor distillation, and its contents were determined using GC/MS. The study determined that the ratio of carvacrol, the main component off Thymbra spicata essential oil changes between %51.14 and 72.54. © 2016 Certex, INCDTP-ICPI.Öğe The effects of different nitrogen doses on Artemisia annua L.(2002) Ayanoglu, Filiz; Mert, Ahmet; Kirici, SalihaThe purpose of this study was to determine the effects of different nitrogen doses (0, 6, 12 and 18 kg/da) on the plant parameters, yields and quality of Artemisia annua L. ecotype from Adana, Turkey. A two-year experiment was conducted between 1996-1998 under Adana ecological conditions. During this study, plant height (cm), number of branches per plant, fresh herb yield (kg/da), dry herb yield (kg/da), dry leaf yield (kg/da), essential oil content (%) essential oil yield (1/da), artemisinin content (%), and artemisinin yield (kg/da) were investigated, The results indicated that application of different N doses statistically affected the essential oil content and oil yield only the first year of the study. But artemisinin content and yield were not significantly affected. © 2002 by The Haworth Press, Inc. All rights reserved.Öğe The effects of different plant densities on yield, yield components and quality of Artemisia annua L. ecotypes(2002) Mert, Ahmet; Kirici, Saliha; Ayanoglu, FilizThis study was conducted between 1997 and 1998 at Çukurova University, Faculty of Agriculture to determine the effects of different plant densities (5, 10, 15, 20, 25 and 30 plants/m2) on yield and yield components and quality of Artemisia annua L. ecotypes (Adana, Samankaya and Serinyol from Turkey). In the study, the plant height (cm), fresh weight (kg/da), dry weight (kg/da), dry leaf weight (kg/da), essential oil content (%), essential oil yield (1/da), artemisinin content (%), and artemisinin yield (kg/da) of ecotypes were investigated. The results of this study showed that the highest essential oil content and the highest essential oil yield were found in the Samankaya ecotype when planted at 15 plants/m2. However, the highest artemisinin content and highest artemisinin yield were found in the Serinyol ecotype when planted at 15 plants/m2. © 2002 by The Haworth Press, Inc. All rights reserved.Öğe Essential Oil and Fatty Acid Composition and Antioxidant Capacity and Total Phenolic Content of Parsley Seeds (Petroselinum crispum) Grown in Hatay Region(Assoc Pharmaceutical Teachers India, 2017) Mert, Ahmet; Timur, MahirThe aim of the present study was to investigate the essential oil and fatty acid compositions and antioxidant capacity and total phenolic content of parsley seeds grown in Arsuz/hatay region. Parsley seeds had a moisture content of 12,6 Qwt.% and ash content of 6,86 wt.% while the essential oil content of 2,52 wt.% and total lipid content of 8,85 wt.%. The essential oil was analyzed by gas chromatography and thirty two volatile compounds were determined. The composition of fatty acids were also investigated by GC and seven content were determined. The seeds were extracted with methanol for determination of antioxidant capacity and total phenolic content. Total phenol concentration was found 67.25 +/- 5.9mg gallic acid equivalent/g of extract powder. DPPH free radical-scavenging activity IC50 was found as 0,523 +/- 12 mg/ml. From the Trolox calibration curve cupric reducing antioxidant capacity TEACCUPRAC: 17,95 mu g / mL was found. Results were compared with standard antioxidant compound BHA and BHT.Öğe Essential oil components of fresh Coriander (Coriandrum sativum L.) herbs from different locations in Turkey(Certex, INCDTP-ICPI, 2016) Turkmen, Musa; Bahadirli, Nadire Pelin; Mert, AhmetFive different Coriander (Coriandrum sativum L.) obtained from different sources were cultivated under Hatay ecological conditions used as a plant material. To determine essential oil rate fresh herbs of coriander were analysed with Neo-Clevenger apparatus by the method steam distillation. Essential oil components were determined by gas chromatography-mass spectrometry (GC-MS) apparatus. Decanal (9.95-16.53), 2-Decanal (3.48-11.86), 1-Decanol (7.67-17.13), Cyclodecanol (4.46-13.78), 2-Dodecenal (5.42-12.65), Cyclododecanal (5.23-7.86) and 13-Tetradecanal (4.87- 11.19) were found main components. © 2016 Certex, INCDTP-ICPI.Öğe Evaluation of some safflower (Carthamus tinctorius L.) cultivars for morpho-agronomic characters under Hatay ecological conditions(1998) Çalışkan, Mehmet Emin; Mert, Ahmet; Mert, Mehmet; İşler, NecmiSome morphological and agronomical characteristics of seven safflower cvs. (Oleicleed, Yerli CK, 308, 5-154, Yenice, Dincer and 416-5) were investigated at autumn sowing under Hatay ecological conditions in 1995-96 and 1996-97 growing seasons. The safflower cultivars differed significantly for all characters studied; but, especially the cultivars Dincer and 5-154 resulted in high seed and oil yield. Based on the results of two years, it was concluded that safflower crop had a growing potential in non-irrigated areas of Hatay region with autumn sowing.Öğe Farklı azot dozlarının kişniş (Coriandrum sativumL.) yaş herba uçucu yağ bileşenleri üzerine etkisi(Hatay Mustafa Kemal Üniversitesi, 2020) Türkmen, Musa; Mert, AhmetAmaç: Bu çalışmada, farklı azot dozlarının (0, 3, 6, 9, 12 kgda-1), Hatay koşullarında yetiştirilen kişniş (Coriandrum sativumvar. vulgare) popülasyonunda taze herba uçucu yağ bileşenleri üzerine etkinliği araştırılmıştır.Yöntem ve Bulgular: Kişniş tohumlarının (29 Ocak 2020) tarlaya ekimi gerçekleştirilmiştir. Azot dozlarının yarısı ekimle birlikte, diğer yarısı ise bitkiler 5–10 cm boylandığında uygulanmıştır. 13 Nisan 2020 tarihinde bitkiler hasat edilmiştir. Taze herba örnekleri 3 saat süre ile Clevenger tipi aparat kullanılarak su buharı distilasyon ile uçucu yağları elde edilmiş ve elde edilen uçucu yağ bileşenlerine GC-MS ile bakılmıştır.Sonuçlara göre ana bileşenler; kontrol parseli ve bütün gübre dozları için; 2-Decenal, 2-Dodecanal, Decanal, Cyclodecanol, 13-Tetradecanal, Dodecanal, 1-Decanol, Undecanol, 2-Tridecenal, olarak tespit edilmiştir.Genel Yorum: Gübre dozlarının artması ile birlikte tespit edilen ana bileşenlerin oranlarının bazılarında artmalar, bazılarında azalmalar gözlemlenirken bir kısmında ise büyük bir değişim gözlemlenmemiştir.Çalışmanın Önemi ve Etkisi: Bu çalışma ile kişniş taze herba uçucu yağındaki etken maddeleri oluşturan bileşenlerin farklı azot dozuna tepkisi belirlenerek en uygun azot dozunun belirlenmesi sağlanmıştır. Bu yönüyle kişniş taze herba uçucu yağı bileşenlerinin antimikrobiyal çalışmalarda kullanılabilirliği, ayrıca farklı bitkilerin uçucu yağ bileşenleri üzerine azot uygulamalarının etkileri konularında yapılacak çalışmalarla da desteklenebileceği düşünülmektedir.Öğe Farklı soğuklama süresi ve kimyasal uygulamaların iki kebere türünde (Capparis spinosa L., Capparis ovata Desf.) tohum çıkışı üzerine etkileri(1999) Ayanoğlu, Filiz; Mert, Ahmet1996 ve 1997 yıllarında yürütülen çalışmalarda iki farklı kebere türüne ait (C. spinosa L. ve C. ovata Desf.) tohumların çıkış oranı üzerine katlama süresi, katlama sıcaklığı ve kimyasal uygulamaların ve bunların kombinasyonlarının etkileri araştırılmıştır. Denemenin 1. yılında C. spinosa L. tohumları üzerinde 10, 20, 30, 40 ve 50 gün 4 °C'de katlama, 50 gün oda sıcaklığında katlama, katlamasız kimyasal uygulama ve hiçbir muameleye tabi tutulmayan kontrol olmak üzere sekiz farklı uygulama denenmiştir. Denemenin II. yılında ise C spinosa L. ve C. ovata Desf. tohumları üzerinde 10, 20, 30, 40 ve 50 gün 4 °C'de katlama, kimyasal uyguladıktan sonra 50 gün 4 °C'de katlama, 50 gün oda sıcaklığında katlama, kimyasal uygulandıktan sonra 50 gün oda sıcaklığında katlama, katlamasız kimyasal uygulama ve hiçbir muameleye tabi tutulmayan kontrol olmak üzere on farklı uygulama denenmiştir. Kimyasal uygulama, tohumların önce 20 dakika konsantre $H_2SO_4$ ile daha sonra ise 1 saat 100 ppm'lik $GA_3$ ile muamele edilmesi şeklinde yapılmıştır. Tohumlar'bütün uygulamalara tabi tutulduktan sonra I. yılda 14 Mart, II.yılda ise 7 Nisan tarihinde fideliklere ekilerek çıkış yüzdeleri belirlenmiştir. Denemeye alınan kebere türlerinden C. ovata'da çıkış yüzdesi C.spinosa'ya göre daha fazla olurken uygulamalar arasında en yüksek çıkış yüzdesi % 52.50 ile-kimyasal uygulama yapılmadan 50 gün süre ile oda sıcaklığında katlamaya alınan C.ovata tohumlarından elde edilmiştir.Öğe Hatay florasında yetişen karabaş lavantanın (Lavandula stoechas subsp. stoechas L.) çelikle köklendirilmesi üzerine farklı lokasyonların ve hormon dozlarının etkisi(2000) Ayanoğlu, Filiz; Mert, Ahmet; Kaya, AlpaslanThe intensive growing areas of Karabaş lavender were first determined in the province of Hatay. The rooting capabilities of the cuttings of Karabaş Lavender which were gathered from different locations, one (Işİklİ) on the coast of Mediterranean and the other one (Narlİca) near Antakya, were investigated. The cuttings were treated with 1 000, 2 000 and 4 000 ppm IBA doses and the cuttings with no IBA treatment were used as control. The effects of IBA on rooting were investigated. The rooting ratios of the cuttings gathered from Işİklİ were higher than those of the cuttings gathered from Narlİca. The hormone doses positively affected the rooting of cuttings gathered from both places, and the rooting ratios, the length of roots and the number of roots per cutting increased with the hormone doses. The highest rooting ratio (70%) was obtained from the cuttings gathered from Işİklİ, treated with 4000 ppm IBA dose.Öğe In vitro cytotoxic activity of Nigella sativa L. On human malignant melanoma cell lines(Certex, INCDTP-ICPI, 2016) Duran, Nizami; Duran, Gulay Gulbol; Ay, Emrah; Kaya, Durmus¸ Alpaslan; Kaya, Madalina Georgiana Albu; Mert, AhmetNigella is a medicinal plant that has various pharmacological properties. It is widely used in folk medicine to treat many diseases in the world. It was aimed to investigate the essential oils Nigella sativa L. on two human melanoma cells (A-375 and A-2058 cell lines). In this study, two different cancer cell lines (A- 375and A-2058 cell lines) and one normal cell culture (vero cells) were used. Firstly, the non-toxic concentrations the essential oils of Nigella sativa L. were determined on normal cells (Vero cell line). And then, the cytotoxicity test were applied at these non-toxic concentrations. The cytotoxic activity of the essential oils of Nigella sativa L. on the cell lines was measured by MTT and Trypan blue assays. The chemical components analyses of the Nigella sativa L. oils were carried out by GC-MS. Medically important various components of Nigella sativa L. were determined in the GC-MS analysis. Some of these components are as follows; anethole (22.97%), thymoquinone (21.36%), a-thujene (6.22%), longifolene (5.76%), trans-isoeugenol (3.55%), carvacrol (2.23%). A total of 24 compounds were identified. The essential oils of Nigella sativa L. were found to inhibit the cell proliferation of human malignant melanoma cells. The IC50 values of the essential oils of Nigella sativa L. It were investigated as compared with a standard drug (methotrexate). Statistically significant decrease on the cell proliferations was found in the cells treated with the essential oils ofNigella sativa L. It was found to be non-toxic on normal cells (on Vero cell line) at the effective concentrations of the essential oils.While the non-toxic concentrations of essential oils on Vero cell line were found to be 12.5 ?g/ml, the effective concentrations for A-375 and A-2058 cell lines were as 1.56 ?g/ml and 3.12 ?g/ml, respectively. In conclusion, the essential oils of Nigella sativa L. were found to be significantly inhibited the cell proliferation on the human melanoma cells. The effect may arise from the components situated in the structure of Nigella sativa L. such asanethole, thymoquinone, a- thujene, longifolene, trans-isoeugenol and carvacrol. These components of this plant have been found promising in the treatment for human melanomas. But further studies, especially further animal studies should be needed to explain the action mechanisms of these components. © 2016 Certex, INCDTP-ICPI.Öğe Propagation of some native grown medicinal plants by stem cuttings(2002) Ayanoglu, Filiz; Mert, Ahmet; Erdogan, Cahit; Kaya, AlpaslanSalvia indica L., Helichrysum plicatum subsp, plicatum D.C., and Satureja thymbra L, are some native grown plants which are used as folk medicine in Hatay province, Turkey. The propagation possibilities of these plants by stem cuttings were investigated, Different IBA (Indole 3-butiric acid) doses were applied to 10-12 cm cuttings as a rooting agent: 100, 250, 500, 1000 and 2000 ppm for H. plicatum; 250, 500, 1000, 2000 and 4000 ppm for S. thymbra; and 100, 250, 500, 1000, 2000 and 4000 ppm for S. indica L. The experimental design was a completely randomized block with four replications. Each replication had 25 cuttings. Cuttings were immersed in different IBA doses for 5 seconds. No IBA was applied to the control cuttings. Rooting ratio (%), root length (cm), root number (number/cutting) and rooting quality of cuttings were measured. The best rooting ratios were obtained from 250 and 1000 ppm IBA doses for H. plicatum, 1000 and 2000 ppm for S. thymbra and S. indica. © 2002 by The Haworth Press, Inc. All rights reserved.Öğe Seed yields, yield components and essential oil of selected coriander (Coriandrum sativum L.) lines(Taylor and Francis Inc., 2002) Ayanoglu, Filiz; Mert, Ahmet; Aslan, Neset; Gürbüz, BilalForty-three coriander lines which were developed by Ankara University were tested under east Mediterranean conditions for two years to determine the best yielding lines in winter season. The experiment was laid out in a randomized complete block design with three replications. During this study, the plant height (cm), branch number/plant, umbel number/plant, seed number/umbel, 1000 seed weight (g), seed yield (kg/da), the essential oil content (%) and the essential oil yield were investigated. The seed yields of coriander lines varied between 113.8 (K11) and 229.7 kg/da (K46). The highest seed yields were obtained from the lines K67, K28, K69 and K46. The seed yields of those lines were higher than currently planted cultivars. © 2002 by The Haworth Press, Inc. All rights reserved.
