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Öğe Beneficial effect of erdosteine on methotrexate-induced testicular toxicity in mice(Sage Publications Inc, 2010) Oktar, Sueleyman; Gokce, Ahmet; Aydin, Mehmet; Davarci, Muersel; Meydan, Sedat; Ozturk, Oktay Hasan; Koc, AhmetMethotrexate is used to treat certain types of cancer of the breast, skin, head and neck, or lung. Methotrexate can cause serious or life-threatening side effects on liver, lungs, kidneys, and immune system. Methotrexate chemotherapy causes testicular damage in humans. The aim of this study was to investigate the possible protective role of erdosteine on testicular toxicity of methotrexate in mice. Twenty-six male mice were divided into four groups as follows: group 1, control; group 2, erdosteine-treated; group 3, methotrexate-treated; and group 4, methotrexate + erdosteine treated. On the first day of experiment, a single dose of methotrexate was intraperitoneally administered to groups 3 and 4, although a daily single dose of erdosteine was orally administered to group 2 and 4 for 7 days. At the end of the experiment, the testes of the animals were removed and weighed. The levels of total antioxidant capacity and total oxidative stress, and myeloperoxidase activity in the methotrexate group were higher than the control group (p<0.05). Lipid peroxidation levels were not changed in methotrexate group compared with control group. In conclusion, erdosteine could effectively protect the testes in methotrexate-induced toxicity.Öğe Damascus kids' slaughter, carcass and meat quality traits in different production systems using antioxidant supplementation(Elsevier, 2016) Yakan, Akin; Ates, Cafer Tayyar; Alasahan, Sema; Odabasioglu, Fuat; Unal, Necmettin; Ozturk, Oktay Hasan; Gungor, Omer FarukThis study intended to investigate slaughter, carcass and meat quality characteristics of Damascus male kids reared under different production systems and antioxidant effect (Vit E). The kids, housed in pen groups and grazing groups, were equally divided for production systems and later each group was again equally divided for determination of Vit E effect. Production systems and Vit E were found to have no significant effect on slaughter and carcass traits. Differences between production systems were found significant for meat pH(24), water holding capacity, cooking loss, tenderness, ether-extractable lipid and some color characteristics and concentrate feed supplemented with Vit E was effective on TBARS values. Each of the fatty acids except C18:2 n6 was affected by the production system but Vit E influence was superior on long-chain fatty acids. Grazing kids had a lower percentage of total SFA, n6, n6/n3, AI and TI ratio, while kids housed in pens had the lowest percentage of total UFA, NV and n3 ratio. On the other hand, kids that consumed supplemental Vit E had a higher percentage of total UFA, ratio of UFA/SFA, n3 and lower percentage of SFA, ratio of n6/n3, AI, TI compared to the kids fed by non-supplemental concentrate feed with Vit E. In accordance with the meat fatty acid composition, meat obtained from the kids that grazed and consumed supplemental Vit E was healthier than that of those housed in pen kids and non-supplemental Vit E consumed kids. (C) 2016 Elsevier B.V. All rights reserved.Öğe Does Polycystic Ovary Syndrome Itself Have Additional Effect on Apelin Levels?(Hindawi Ltd, 2014) Silfeler, Dilek Benk; Gokce, Cumali; Kurt, Raziye Keskin; Atilgan, Nigar Yilmaz; Ozturk, Oktay Hasan; Turhan, Ebru; Baloglu, AliObjective. The present study was designed to compare serum levels of apelin between lean PCOS women and healthy women with regular menses. Study Design. A total of 30 lean patients with PCOS and 30 healthy subjects were included in this study. Serum apelin levels were compared between groups. Results. Serum apelin levels in lean PCOS patients were not significantly different from the control subjects. Conclusion. Our findings indicate that PCOS itself does not seem to change apelin levels. Further investigation on a large number of subjects will need to be conducted to prove the consistent or variable association in PCOS.Öğe Dual effects of erdosteine on hemostasis via its different metabolites in young rats(Sage Publications Ltd, 2011) Arica, Vefik; Tutanc, Murat; Ozturk, Oktay Hasan; Arica, Secil; Basarslan, Fatmagul; Erden, Ersin Sukru; Oktar, SuleymanAim: In the study, we examined erdosteine's effects on platelet functions and coagulation. Materials and methods: A total 29 young albino Wistar rats were divided into four groups. Control rats (n = 6) were given saline; Group I rats (n = 7) were given 3 mg/kg erdosteine by oral gavage for 3 days; Group 2 rats (n = 7) were given 10 mg/kg erdosteine by oral gavage for 3 days; and Group 3 rats (n = 9) were given 30 mg/kg erdosteine for 3 days. Twenty-four hours after the final dose, blood samples were drawn from a portal vein. Prothrombin time (PT), activated partial thromboplastin time (aPTT) and international normalized ratio (INR) were measured, and platelet counts were examined in a peripheral blood smear by light microscopy. Results: PT and INR values of Group I increased compared to the controls but did not change in Group 3. Hemostatic parameters were not measured in Group 2 because the blood samples in Group 2's tubes clotted rapidly. Platelet counts of the peripheral blood from Group 2 were low but were normal in other groups. Conclusion: We have concluded erdosteine may disrupt hemostasis parameters by its different metabolites in patients. Erdosteine has dual effects on hemostasis via its different metabolites, which occur in different doses.Öğe Effect of sulfite on antioxidant enzymes and lipid peroxidation in normal and sulfite oxidase-deficient rat erythrocytes(Springer, 2010) Ozturk, Oktay Hasan; Oktar, Suleyman; Aydin, Mehmet; Kucukatay, VuralSulfite and related chemical such as sulfite salts and sulfur dioxide has been used as a preservative in food and drugs. This molecule has also been generated from the catabolism of sulfur-containing amino acids. Sulfite is a very reactive and potentially toxic molecule and has to be detoxified by the enzyme sulfite oxidase (SOX). The aim of this study was to investigate the effects of ingested sulfite on erythrocyte antioxidant status by measuring glucose-6-phosphate dehydrogenase (G-6-PD), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activities and oxidant status by measuring thiobarbituric acid reactive substances (TBARS) in normal and SOX-deficient rats. Rats were assigned to four groups (n = 10 rats/group) as follows; control (C), sulfite (CS), deficient (D), and deficient+sulfite (DS). SOX deficiency was established by feeding rats a low molybdenum diet and adding to their drinking water 200 ppm tungsten (W). Sulfite (25 mg/kg) was administered to the animals via their drinking water. At the end of 6 weeks, Erythrocyte G-6-PD, SOD, and GPx but not CAT activities were found to be significantly increased with and without sulfite treatment in SOX-deficient groups. Sulfite treatment alone was also significantly increased erythrocytes' SOD activity in CS group compared to control. TBARS levels were found to be significantly increased in CS and DS groups and decreased in D group. When SOX-deficient rats treated with sulfite, TBARS level was still higher than other groups. In conclusion, these results suggested that erythrocyte antioxidant capacity, a defense mechanism against the oxidative challenge, increased by endogenous and exogenous sulfite due to its oxidant nature. This increase was also observed in CS and DS groups but it was insufficient to prevent lipid peroxidation.Öğe Effect of Thymoquinone on Oxidative Stress in Escherichia coli-Induced Pyelonephritis in Rats(Elsevier Science Inc, 2011) Evirgen, Omer; Gokce, Ahmet; Ozturk, Oktay Hasan; Nacar, Emel; Onlen, Yusuf; Ozer, Burcin; Motor, Vicdan KoksaldiBACKGROUND: Recurrent urinary tract infections are important in children and adults with diabetes mellitus and/or incontinence due to risk of pyelonephritis (PYN) and renal damage. There is a positive correlation released free radicals during PYN and renal damage. Experimental studies showed that antioxidant agents improve renal damage when used immediately after bacterial inoculation. OBJECTIVE: The aim of the present study was to evaluate whether treatment by thymoquinone (TQ) before or during Escherichia coli inoculation prevents oxidative damage in acute pyelonephritis (PYN) in an ascending obstructive rat model. METHODS: In this study, 42 Wistar rats were grouped as follows: control, PYN (24, 48, and 72 hours), and TQ-PYN (24, 48, and 72 hours). E. coli (1 x 10(9) colony forming units) was inoculated into the bladder via urethral catheterization in both the PYN and TQ groups. TQ injections were performed 24 hours before bacteria inoculation and repeated at 24-hour intervals during the indicated time at a dose of 10 mg/kg body weight intraperitoneally in TQ groups. RESULTS: Superoxide dismutase activity was statistically lower in the TQ-PYN-48 and -72 groups than the PYN-48 and -72 groups (P < 0.001, P = 0.004, respectively). Catalase activity was significantly higher in PYN-24, -48, and -72 groups than the control group (P < 0.001). In addition, there was a significant difference between the TQ-PYN-24, -48, and -72 groups and PYN groups in terms of glutathione peroxidase activity (P < 0.001, P = 0.026, P = 0.046, respectively). When the TQ-PYN-72 group was compared with the PYN-72 group, malondialdehyde levels were significantly lower in the TQ-PYN-72 group than in the PYN-72 group (P = 0.033). A histologic examination also confirmed the protective effect of TQ. In statistical analysis of histopathologic findings, there were significant differences between the PYN-24 and TQ-PYN-24, PYN-48 and TQ-PYN-48, and PYN-72 and TQ-PYN-72 groups (P = 0.008, P < 0.001, P < 0.001, respectively). CONCLUSIONS: The results indicate that TQ administration attenuated the oxidative damage that occurred in PYN and, therefore, could be used as a supportive agent to protect the kidneys from oxidative damage caused by PYN. (Curr Ther Res Clin Exp. 2011;72:204-215) (C) 2011 Elsevier HS Journals, Inc. All rights reserved.Öğe Effects of ?-Glucan Pretreatment on Acetylsalicylic Acid-Induced Gastric Damage: An Experimental Study in Rats(Elsevier Science Inc, 2010) Ozkan, Orhan Veli; Ozturk, Oktay Hasan; Aydin, Mehmet; Yilmaz, Nigar; Yetim, Ibrahim; Nacar, Ahmet; Oktar, SuleymanBACKGROUND: NSAIDs have been found to induce gastrointestinal tract damage. Recently, it has been suggested that this might be mediated by lipid peroxidation. OBJECTIVE: The aim of this study was to assess the potential protective effects of beta-glucan against acetylsalicylic acid (ASA)-induced gastric damage by means of its antioxidant capacity in an experimental rat model. METHODS: Thirty-two male Wistar albino rats (200-250 g) were randomized into 4 groups consisting of 8 rats each. The beta-glucan group received 50 mg/kg beta-glucan once a day for 10 days and 30 minutes before anesthesia. The ASA group received saline once a day for 10 days and 300 mg/kg (20 mg/mL) ASA as a single dose, 4 hours before anesthesia. The ASA+beta-glucan group was administered 50 mg/kg beta-glucan once a day for 10 days and 30 minutes before anesthesia. Additionally, 300 mg/kg (20 mg/mL) ASA was administered as a single dose, 4 hours before anesthesia. The control group received saline once a day for 10 days and 30 minutes before anesthesia. All medications were administered by intragastric gavage. The stomach from each rat was dissected and divided into 2 parts for histologic and biochemical analysis. Gastric tissue malondialdehyde (MDA), nitric oxide (NO) levels, catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) activities were determined for oxidative parameter analysis. RESULTS: The gastroprotective and antioxidant effects of beta-glucan appeared to attenuate the ASA-induced gastric tissue damage. Compared with the control group, MDA and NO levels and CAT and GSH-Px activities were significantly increased in the stomachs of ASA-treated rats (MDA, 4.12 [0.44] to 13.41 [1.05] mu mol/L; NO, 8.04 [7.25-9.10] vs 30.35 [22.34-37.95] mu mol/g protein; CAT, 0.050 [0.004] to 0.083 [0.003] k/g protein; GSH-Px, 0.57 [0.42-0.66] to 1.55 [1.19-1.76] U/L; all, P < 0.001), whereas SOD activity was significantly decreased in the same group (291 [29] to 124 [61 U/mL; P < 0.001). In the ASA+beta-glucan group, MDA and NO levels and CAT and GSH-Px activities were found to be significantly lower, while SOD activity was found to be significantly higher, in comparison with the ASA-treated group (all, P < 0.001). CONCLUSION: beta-Glucan appeared to attenuate the gastric damage caused by ASA in these rats. (Curr Ther Res Clin Exp. 2010;71:369-383) (C) 2010 Elsevier HS Journals, Inc.Öğe THE EFFECTS OF L-CARNITINE IN BUDD-CHIARI SYNDROME IN A DOMESTIC CAT(Univ Sv Kiril & Metodij Skopje, Fak Veterinarna Medicina, 2016) Ozturk, Aliye Sagkan; Altug, Nuri; Kose, Serkan Irfan; Ozturk, Oktay HasanThis paper describes a thrombosis in the vena cava caudalis of a 15 year-old cat with ascites. Trauma and eventually feline enteric corona virus infection in the cat were not detected. In the intrahepatic region, a blockage of vena cava caudalis was brought to light by ultrasonographic imaging. An aspirate of abdominal fluid revealed modified transudate. Liver enzyme levels were increased in the serum sample of the cat. The levels of total oxidant status (TOS) and total antioxidant status (TAS) were elevated in the peritoneal fluid. Liver protection diet with L-carnitine, diuretic therapy and antimicrobial drugs were administrated for treatment of the cat. During the continuous treatment, the amount of abdominal fluid decreased, but never completely absorbed. L-carnitine was administered to the cat during the time of treatment, and subsequently the levels of liver enzymes decreased. However, the cat died because of recurrent ascites and persistent thrombosis. In conclusion, ultrasonographic examination was very reliable, non-invasive and highly useful diagnostic method for BCS and L-carnitine has crucial effects on the quality of life, energy metabolism and liver enzyme levels. However, the blockage of the vena cava caudalis could not completely respond to medical treatment and thrombosis should be eliminated by surgical intervention.Öğe Erdosteine has dual effects on haemostasis via its different metabolites in young rats(Taylor & Francis Inc, 2010) Oktar, Sueleyman; Arica, Vefik; Tutanc, Murat; Ozturk, Oktay Hasan[Abstract Not Available]Öğe Evaluation of oxidative stress, antioxidant status and lipid profile in cattle with displacement of the abomasum(Ankara Univ Press, 2016) Durgut, Ramazan; Sagkan Ozturk, Aliye; Ozturk, Oktay Hasan; Guzel, MuratThe aims of the present study were to evaluate serum oxidative and antioxidant status and oxidative stress index, and lipid metabolism profile in cattle with abomasal displacement. A total of thirty-two dairy cows with right-and left-sided abomasal displacement (LDA (n=11) and RDA without volvulus (n=11)) and 10 healthy controls were used in this study. Serum total oxidative status (TOS) and total antioxidant capacity (TAC) were measured by a colorimetric method. The TOS-to-TAC ratio was also calculated as oxidative stress index (OSI) value. Serum lipid profiles were determined by conventional measurement methods as well. Mean TOS and OSI values were significantly (p<0.001) higher in cows with RDA compared to LDA and healthy controls; however, there was no significant differences in serum TAC values among groups. Serum total cholesterol (TC), high density lipoproteins (HDL) and low density lipoproteins (LDL) levels were significantly (p=0.001) decreased, while serum triglyceride (TG) levels were significantly increased in dairy cows with right-and left-sided abomasal displacement compared to healthy controls. The results of current study showed that dairy cows with RDA forming free radicals than LDA and controls due to abomasal tension and increased intraluminal pressure.Öğe Evaluation of oxidative stress, antioxidant status and lipid profile in cattle with displacement of the abomasums(Chartered Inst. of Building Services Engineers, 2016) Durgut, Ramazan; Sagkan Ozturk, Aliye; Ozturk, Oktay Hasan; Guzel, MuratThe aims of the present study were to evaluate serum oxidative and antioxidant status and oxidative stress index, and lipid metabolism profile in cattle with abomasal displacement. A total of thirty-two dairy cows with right- and left-sided abomasal displacement (LDA (n=11) and RDA without volvulus (n=11)) and 10 healthy controls were used in this study. Serum total oxidative status (TOS) and total antioxidant capacity (TAC) were measured by a colorimetric method. The TOS-to-TAC ratio was also calculated as oxidative stress index (OSI) value. Serum lipid profiles were determined by conventional measurement methods as well. Mean TOS and OSI values were significantly (p<0.001) higher in cows with RDA compared to LDA and healthy controls; however, there was no significant differences in serum TAC values among groups. Serum total cholesterol (TC), high density lipoproteins (HDL) and low density lipoproteins (LDL) levels were significantly (p=0.001) decreased, while serum triglyceride (TG) levels were significantly increased in dairy cows with right- and left-sided abomasal displacement compared to healthy controls. The results of current study showed that dairy cows with RDA forming free radicals than LDA and controls due to abomasal tension and increased intraluminal pressure. © 2016, Chartered Inst. of Building Services Engineers. All rights reserved.Öğe Frequency of MEFV gene mutations in Hatay province, Mediterranean region of Turkey and report of a novel missense mutation (I247V)(Elsevier, 2014) Gunesacar, Ramazan; Celik, Muhammet Murat; Arica, Vefik; Elmacioglu, Sibel; Ozturk, Oktay HasanIn the present study, 1000 patients with clinical suspicion of FMF were retrospectively reviewed to determine the spectrum of MEFV gene mutations by using DNA sequence analysis between September, 2008 and April, 2012. Sixteen different mutations and 55 different genotypes were detected in 618 of 1000 patients. Among 16 different mutations, R202Q (21.35%) was the most frequently observed mutation; followed by E148Q (8.85%), M694V (7.95%), M680I (2.40%), V726A (1.85%), M694I (0.95%), A744S (0.80%), R761H (0.55%), P283L (0.35%), K695R (0.20%), E230K (0.15%), L110P (0.10%), I247V (0.05%), G196W (0.05%) and G304R (0.05%). In the present study, a novel missense mutation (I247V) and a silent variant (G150G) were identified in the MEW gene. On the other hand, P238L, G632A and G304R mutations are the first cases reported from Turkey. Our results indicated that MEW mutations are highly heterogeneous in our study population as in other regions of Turkey and mutation screening techniques such as PCR-RFLP, amplification refractory mutation system or reverse hybridization do not adequately detect uncommon or novel mutations. Therefore, it was proven that sequence analysis of the MEW gene could be useful for detection of rare or unknown mutations. (C) 2014 Elsevier B.V. All rights reserved.Öğe High plasma nesfatin-1 level in patients with major depressive disorder(Pergamon-Elsevier Science Ltd, 2011) Ari, Mustafa; Ozturk, Oktay Hasan; Bez, Yasin; Oktar, Suleyman; Erduran, DuduAim: In the present study, our aim was to determine the changes in the plasma concentrations of a recently discovered peptide hormone nesfatin-1 in patients with major depressive disorder and then to make a comparison with the control group. Method: Subjects in the patient group were randomly selected from Mustafa Kemal University, Medical School, Research and Training Hospital, Psychiatry Department, Outpatient Clinic and subjects in the control group were selected from healthy volunteers. Healthy control subjects were matched in terms of weight and body mass index. Hamilton Depression Rating Scale (HAM-D) was applied to both groups. ELISA method was used for measurement of plasma nesfatin-1 levels. Results: The average nesfatin-1 level was statistically higher in patients with major depressive disorder than in the control group (p<0.001). A positive correlation was observed between plasma nesfatin-1 levels and HAM-D scores both in the patient group (r = 0.59, p<0.001) and in the control group (r = 0.58, p<0.001). Conclusion: Our findings suggest a possible relationship between major depressive disorder and high plasma nesfatin-1 level. (C) 2010 Elsevier Inc. All rights reserved.Öğe Increased Plasma Nesfatin-1 Levels in Patients with Obsessive Compulsive Disorder(Kure Iletisim Grubu A S, 2012) Bez, Yasin; Ari, Mustafa; Ozturk, Oktay Hasan; Oktar, Suleyman; Can, YesimIncreased plasma nesfatin-1 levels in patients with obsessive compulsive disorder Objective:To determine and compare the plasma nesfatin-1 (a satiety peptide) levels of patients with obsessive compulsive disorder (OCD) and healthy control subjects. Method: Plasma nesfatin-1 levels of 31 patients with OCD (18 females, 13 males) and 28 healthy control subjects (16 females and 12 males) similar to the study group in terms of weight, age, and gender were measured in this study. Severity of obsessions and compulsions both in OCD patients and control subjects were determined by using Yale-Brown Obsessive Compulsive Scale (Y-BOCS). ELISA method was used to measure plasma nesfatin-1 levels. Results: Median plasma nesfatin-1 levels in patients with OCD and healthy control subjects were 4.61 ng/ml (min-max: 1.28-8.11) and 2.0 ng/ml (min-max: 0.11-4.98) respectively. The observed difference in plasma nesfatin-1 levels between two groups was statistically significant (p<0.001). No statistically significant correlation was observed between Y-BOCS scores and plasma nesfatin-1 levels either in the study group (r=0.205, p=0.27) or in the control group (r=0.335, p=0.071). Conclusion: Increased plasma nesfatin-1 levels observed in patients with OCD suggest a potential role to nesfatin-1 in anxiety states besides its previosly known anorexigenic effects.Öğe Investigation of the clinical and hematological significance of the first observed hemoglobin Ernz variant [?123(H1) Thr>Asn] in the Turkish population(Tubitak Scientific & Technological Research Council Turkey, 2012) Gunesacar, Ramazan; Celik, Muhammet Murat; Ozturk, Oktay Hasan; Celik, Mustafa; Tumer, Cemil; Celik, TanjuAim: In this report, we aimed to investigate the clinical and hematological significance of the first observed hemoglobin Ernz variant in the Turkish population. Materials and methods: We identified the Hb Ernz variant in 3 nonrelated females (Probands 1, 2, and 3). Proband l's family was also included the study. Hematological data were obtained with an automated cell counter and routine methodology. The beta-globin gene was sequenced by automatic sequencing. Results: Proband 1 was detected as a combination of Hb Ernz/Hb S without any clinical symptoms. Her sister and brother had to be an Hb Ernz/Hb S combination. Her mother and father only showed Hb Ernz and Hb S, respectively. Proband 2 had the Hb Ernz variant with IVS-I 5nt homozygous alpha 2 gene mutation. Proband 3 had a heterozygous Hb Ernz variant. All subjects were clinically and hematologically normal but Proband 2 had low hemoglobin, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, and high red blood cell distribution width levels. Conclusion: In the present study, the Hb Ernz variant is demonstrated for the first time in the Turkish population. Additionally, there is no published report in the world literature of Hb Ernz in combination with IVS-I 5nt homozygote mutation in the alpha-globin gene or Hb S variant. The present report shows that the Hb Ernz variant is not clinically or hematologically significant.Öğe Iodine, copper, zinc, selenium and molybdenum levels in children aged between 6 and 12 years in the rural area with iodine deficiency and in the city center without iodine deficiency in Hatay(Aves, 2014) Celik, Tanju; Savas, Nazan; Kurtoglu, Selim; Sangun, Ozlem; Aydin, Zeki; Mustafa, Didin; Ozturk, Oktay HasanAim: Thyroid diseases related with iodine deficiency are observed commonly in our country and in the world. In this study, we aimed to investigate iodine deficiency in urine and selenium, zinc, copper or molybdenum deficiencies which may accompany this in children aged between 6 and 12 years in two schools in the province of Hatay (endemic goitre region). Material and Methods: This study is a case-control field-study in which students aged between 6 and 12 years were included. One hundred fourteen subjects from the village of Tanisma related to the center of our province and 100 subjects from the city center of Hatay (Antakya) were included in the study. Iodine, selenium, zinc, copper and molybdenum levels were measured in the urine samples of the students included in the study. Results: Iodine deficiency was found with a severe (5%), moderate (18.4%) and mild degree (43%), respectively in the village of Tanisma. Mild iodine deficiency (7%) was found in the center of Hatay. No moderate and severe iodine deficiency was found in the control group. A significant difference was found between the groups in terms of urine iodine excretion (p < 0.001). A significant correlation was found between the levels of iodine, selenium, zinc and molybdenum (p < 0.05). A moderately positive correlation was found between iodine and selenium (p < 0.001). A moderately positive correlation was found between iodine and zinc levels (p < 0.001) and a weak correlation was found between iodine and molybdenum (p < 0.01). No significant correlation was found between iodine level and copper level (p > 0.05). Conclusions: Selenium and zinc deficiency may accompany iodine deficiency. Selenium and zinc deficiency should be considered in individuals who are found to have iodine deficiency especially in endemic goitre regions.Öğe Letrozole induces hepatotoxicity without causing oxidative stress: the protective effect of melatonin(Taylor & Francis Ltd, 2011) Aydin, Mehmet; Oktar, Suleyman; Ozkan, Orhan Veli; Alcin, Ergul; Ozturk, Oktay Hasan; Nacar, AhmetAim. The aim of this study was to determine the effects of letrozole (LTZ), an aromatase inhibitor (AI), and melatonin (MLT) on hepatic function and oxidative stress in female rats. Material and methods. A total of 32 female rats were divided equally into four groups (n = 8). Control group received saline (0.5 ml/day, oral gavage). LTZ was administered to rats by daily oral gavage at 1 mg/kg dose. LTZ + MLT group was given LTZ (1 mg/kg, oral gavage) plus MLT (0.5 mg/kg/day, s.c.). MLT group was given MLT (0.5 mg/kg/day) by s.c. injection. The activities of superoxide dismutase (SOD) and catalase (CAT) and malondialdehyde (MDA) levels were measured in liver tissue. Total antioxidant capacity (TAC), total oxidant status (TOS), ALT, AST, GGT, ALP, LDH, bilirubin, BUN, creatinine, total cholesterol (TC), high-density lipoprotein (HDL) and triglyceride (TG) were assayed in serum samples. Results. The oxidative stress, parameters did not differ between groups. LTZ administration increased hepatic function parameters such as AST, LDH, ALP, bilirubin and MLT improved the disturbances of hepatic function. LTZ caused minimal histological changes in liver tissue and MLT treatment reversed those dejenerations. Discussion. LTZ may cause hepatotoxicity without inducing oxidative stress and MLT restores hepatic activity.Öğe Oxidative Status in Epileptic Children Using Carbamazepine(Briefland, 2015) Tutanc, Murat; Aras, Mustafa; Dokuyucu, Recep; Altas, Murat; Zeren, Cem; Arica, Vefik; Ozturk, Oktay HasanBackground: There is an increasing attention towards the relationship between oxidative stress and epilepsy. The effect of antiepileptic drugs on oxidant status is of major interest. Antiepileptic drugs can increase levels of free radicals, which consequently might lead to seizures. Carbamazepine (CBZ) is an antiepileptic drug commonly used in childhood and adolescence. Objectives: Therefore we aimed to investigate the effects of CBZ on total antioxidant status, total oxidant stress, and oxidative stress index. Patients and Methods: The study included 40 epileptic patients and 31 healthy children between 4 and 12 years of age. Serum CBZ level, total antioxidant capacity and total oxidant status were measured. Oxidative stress index was also calculated both in controls and patients. Results: In the epileptic group, decreased levels of total antioxidant capacity, increased total oxidative stress and oxidative stress index levels were found. Positive correlation between plasma CBZ levels and total oxidant status was observed. Conclusions: Antioxidant action could not be playing any role in antiepileptic effect of CBZ. Furthermore, increased oxidative stress induced by CBZ could be the cause of CBZ-induced seizures. Therefore combining CBZ with antioxidants could be beneficial.Öğe PDGF-? receptor and PKC have no effect on angiotensin II-induced JAK2 and STAT1 phosphorylation in vascular smooth muscle cells under high glucose condition(Taylor & Francis Ltd, 2011) Ozturk, Oktay Hasan; Cetin, Arzu; Tokay, Alper; Uzuner, Fatih; Tanriover, Gamze; Yesilkaya, AkinBackground: The mechanisms responsible for the accelerated cardiovascular disease in diabetes, as well as the increased hypertrophic effects of angiotensin II (Ang II) under hyperglycemic condition, are not very clear. Evidences show that platelet-derived growth factor (PDGF) and protein kinase C (PKC) play a critical role in this effect. In our study, we examined the role of PKC and PDGF receptor on JAK2 and STAT1 phosphorylation under high glucose (HG) condition (25 mmol/L) in response to Ang II in cultured vascular smooth muscle cells (VSMC). Methods: VSMCs were isolated from the thoracic aorta of male Wistar rats and were cultured. Growth-arrested VSMCs were placed in either normal glucose (NG) or HG condition for 48 h and then VSMCs were stimulated with agonists and antagonists. The tyrosine phosphorylation of JAK2 or STAT were determined by immunoblotting using specific antibodies. Results: High glucose markedly increased the phosphorylation of tyrosine residues of JAK2 and serine residues of STAT 1 compared with cells cultured in NG (5.5 mmol/L) with and without Ang II stimulation. Experiments made with specific PDGF-beta receptor inhibitor AG1295 and PKC inhibitor GF109203X showed that there were no changes in Ang II-stimulated JAK2 and STAT1 phosphorylation under NG and HG conditions compared with experiments without inhibitors. Conclusion: According to our findings, Ang II-stimulated JAK2 and STAT1 phosphorylation under either NG or HG condition do not proceed via a different pathway rather than PKC and PDGF-beta receptor.Öğe Prognostic significance of neutrophil gelatinase-associated lipocalin in St-segment elevation myocardial infarction(BMJ Publishing Group, 2012) Akcay, Adnan Burak; Ozlu, Mehmet Fatih; Sen, Nihat; Cay, Serkan; Ozturk, Oktay Hasan; YalNcn, Fatih; Bilen, PerihanObjectives: This study investigated the prognostic value of neutrophil gelatinaseYassociated lipocalin (NGAL) in patients with ST-segment elevation myocardial infarction (STEMI). Background: Neutrophil gelatinaseYassociated lipocalin is a promising biomarker for acute kidney injury. Recently, it was concluded that NGAL may be used beyond the boundaries of renal physiopathology. It was found to be an important factor indirectly contributing to the inflammatory processes. Little is known regarding its predictive role in STEMI. Methods: One hundred six consecutive patients who underwent percutaneous coronary intervention (PCI) for STEMI and control group consisted of age- and sex-matched 60 consecutive patients with chest pain admitted to the hospital for elective PCI. According to median NGAL level, patients were classified into high- and low-NGAL groups. Results: Neutrophil gelatinaseYassociated lipocalin levels were higher in patients with STEMI compared to the elective PCI group subjects. Inhospital and 1-year mortality rates were found to be significantly greater in patients with high NGAL. In addition, inhospital and 1-year major adverse cardiovascular event rates were significantly greater in the high-NGAL group, compared to the low NGAL group. High NGAL level on admission was a significant predictor for long-term mortality and major adverse cardiovascular events. The receiver operating characteristics curve analysis further illustrated that NGAL level on admission is a strong indicator of mortality, with an area under the curve of 0.76 (95% confidence interval, 0.62-0.89). Conclusions: High NGAL levels may be associated with poor prognosis after PCI in patients with STEMI. However, further studies with larger numbers of patients and longer follow-up are required to evaluate the usefulness of plasma NGAL level for predicting prognosis of STEMI. © 2012 by The American Federation for Medical Research.
