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Öğe Antibiotic susceptibility and molecular identification of antibiotic resistance genes of staphylococci isolated from bovine mastitis in Algeria(2015) Saidi, Radhwane; Cantekin, Zafer; Khelef, Djamel; Ergün, Yaşar; Solmaz, Hasan; Kaidi, RachidBu çalışma, Cezayir’de süt sığırlarındaki mastitis vakalarından izole edilen 21 stafilokok (10 Staphylococcus aureus ve 11 Koagulaz Negatif Stafilokok) suşunun Cezayir’de veteriner sahada sıklıkla kullanılan 12 antibiyotiğe karşı in vitro fenotipik ve genotipik direncinin belirlenmesi amacıyla yapıldı. Stafilokok izolatları disk difüzyon yöntemiyle test edildi. mecA, blaZ, aac-aph, ermA, ermC, tetK ve tetM direnç genleri ise PCR ile araştırıldı. Stafilokok izolatları penisilin (%95.23), oksasilin (%80.95), klindamisin (%80.95) ve eritromisine (%76.19) karşı yüksek oranda dirençli bulundu. 21 stafilokok izolatından 20 tanesinin metisilin dirençli ve çoklu antibiyotik direncine sahip olduğu belirlendi. Çoklu antibiyotik direncine sahip suşların bir çok antibiyotiğe karşı direnç paterni belirlendi antibiyotik I-XIII). Antibiyotik direnç genlerinin oranları ise mecA (%100), tetM (%100), blaZ (%42.85) şeklinde gerçekleşti. Bu çalışmada çoklu antibiyotik direncine de sahip metisilin dirençli stafilokokların yüksek prevalansı dikkat çekici idi. Bu çalışma Cezayir’de sığır mastitislerinden izole edilen stafilokoklarda metisilin direncini ortaya koyan ilk çalışmadır. Bu direncin ortaya konulması sürü bazında hastalığın tedavi, kontrol ve mastitis nedeniyle sürüden ayrılacak hayvanlar için karar verilmesine yardımcı olabilir.Öğe Antibiotic Susceptibility and Molecular Identification of Antibiotic Resistance Genes of Staphylococci Isolated from Bovine Mastitis in Algeria(Kafkas Univ, Veteriner Fakultesi Dergisi, 2015) Saidi, Radhwane; Cantekin, Zafer; Khelef, Djamel; Ergun, Yasar; Solmaz, Hasan; Kaidi, RachidThe study aimed to investigate the phenotypic and genotypic identification of in vitro antimicrobial susceptibility of 21 Staphylococci (10 Staphylococcus aureus and 11 Coagulase Negative Staphylococci) isolated from bovine mastitis to 12 antimicrobial drugs frequently using in veterinary medicine in Algeria. Isolates of staphylococci from bovine mastitis were tested for antibiotics with disc-diffusion method according to the National Committee for Clinical Laboratory Standards guidelines in the Mueller-Hinton agar, and resistant genes mecA, blaZ, aac-aph, ermA, ermC, tetK and tetM were detected by PCR. Staphylococci isolates showed high resistance to penicillin (95.23%), oxacillin (80.95%), clindamycine (80.95%), and erythromycin (76.19%) but, no resistance in all these strains was detected for gentamicin. Among 21 isolates of Staphylococci, 20 were found to be methicillin and multidrug resistant. Multidrug resistant strains exhibited several antibiogram patterns (antibiotic I to XIII). The distribution of antibiotic-resistant genes was mecA (100%) and tetM (100) followed by blaZ (42.85%). In the present study, the significant determination was the high prevalence of methicillin-resistant Staphylococci, which were resistant to multiple antibiotics. The finding of methicillin-resistant staphylococci from bovine mastitis is the first report in Algeria and revealed the status of resistant isolates in herd that might be helpful in treatment, controlling of resistant strains and for deciding culling of cows.Öğe Antibody detection against Akabane (AKA) and Bluetongue (BT) viruses in Algerian dromedary camels(TÜBİTAK, 2020) Saidi, Radhwane; Doğan, Fırat; Ataseven, Veysel Soydal; Ergün, YaşarIn this study, blood samples collected from dromedary camels in the Algerian provinces of Laghouat and Ghardaia, which were kept in the same pastures and in the same herds with sheep and goats, were examined to detect antibodies to the Bluetongue (BT) and Akabane (AKA) viruses using enzyme linked immunosorbent assay and serum neutralization tests, respectively. Overall, 16 (14.4%) and 2 (1.8%) of 111 tested camel serum samples were seropositive for the BT virus (BTV) and the AKA virus (AKAV), respectively. The present study determined that BTV infection was prevalent in camels in Algeria, and antibodies to the AKAV were also detected for the first time in Algerian camels.Öğe Bovine herpesvirus-1 (BHV-1), bovine leukemia virus (BLV) and bovine viral diarrhea virus (BVDV) infections in Algerian dromedary camels (Camelus dromaderius)(Springer, 2018) Saidi, Radhwane; Bessas, Amina; Bitam, Idir; Ergun, Yasar; Ataseven, Veysel SoydalThis study was performed to investigate the presence of bovine herpesvirus-1 (BHV-1), bovine leukemia virus (BLV) and bovine viral diarrhea virus (BVDV) infections in dromedary camels (Camelus dromaderius) kept in mixed herds with sheep and goats in Algeria, since the prevalence of BHV-1, BVDV, and BLV infections among dromedary camels in Algeria is unknown. Totally, 111 camel sera were collected from two provinces (Laghouat and Ghardaia) in Algeria. The sera were analyzed for BHV-1 specific antibodies, BVDV specific antibodies and BVDV antigen using the ELISA, and BLV nucleic acid using PCR. The seropositivity rate was 9.0% for BVDV-specific antibody, although 41.4% of camels tested were positive for BVDV antigen. Moreover, there was no evidence of BHV-1 and BLV infections. The results indicated that camels might represent an important source for BVDV infection in all ruminants, including cattle, sheep, and goats bred in mixed herds in Algeria, since they had a higher BVDV prevalence rates. Therefore, the prevention and control measures for BVDV infection should put in place in camel populations to limit the spread of BVDV infection to ruminant populations in Algeria.Öğe A Duplex PCR for detection of S. aureus and Staphylococcus spp. from culture and bovine milk samples(2014) Cantekin, Zafer; Saidi, Radhwane; Solmaz, Hasan; Ergün, YaşarMastitis süt sığırcılığı endüstrisinin en fazla ekonomik kayba neden olan problemidir. Stafilokok türleri en sık izole edilen etkenler olup, Staphylococcus aureus ise süt sığırlarında en önemli kontagiyöz etkendir. Bu çalışmanın amacı stafilokok türleri ve S. aureus’un kültür ve süt örneklerinden teşhisi için ikili bir PZR tekniğinin geliştirilmesidir. Çalışmada pozitif kontrol olarak, S. aureus (ATCC 25923) ve S. epidermidis (ATCC 12228) DNA’sı kullanıldı. Geliştirilen tekniğin denenmesi amacıyla Veteriner Hekimlerce laboratuvarımıza gönderilen örneklerden bir adet koagulaz negatif stafilokok bir adet de S. aureus pozitif süt örneği klinik örnek olarak kullanıldı. Çalışmada sütten ve kültür örneklerinden stafilokok ve S. aureus’un teşhisi ve ayrımı için ikili ve hızlı bir PZR tekniği geliştirildi. Geliştirilen bu tekniğin stafilokok türleri ve S. aureus’un bir kaç saat içinde hem süt hem de kültür örneklerinden tanısı ve ayrımı için alternatif, güvenilir ve hızlı bir tanı yöntemi olarak özellikle sonuçların çiftlik bazında kullanılmak üzere klinik ve subklinik olgularda ineğin tedavi edilmesi ya da kesime gönderilmesine karar verilmesi amacıyla kullanılabileceği ortaya konuldu.Öğe PCR ASSAY WITH HOST SPECIFIC INTERNAL CONTROL FOR STAPHYLOCOCCUS AUREUS FROM BOVINE MILK SAMPLES(Univ Sv Kiril & Metodij Skopje, Fak Veterinarna Medicina, 2015) Cantekin, Zafer; Ergun, Yasar; Solmaz, Hasan; Ozmen, Gamze Ozge; Demir, Melek; Saidi, RadhwaneStaphylococcus aureus is considered as one of the most important and common pathogens of bovine mastitis. Polymerase Chain Reaction is frequently proposed in the diagnosis of S. aureus directly from milk samples instead of classical culture. However, false-negative results may occur in the polymerase chain reaction analysis performed directly from clinical material. For the purpose of disclosing the false negative results, the use of internal amplification controls can be beneficial. Therefore, in this study a new polymerase chain reaction technique with host specific internal amplification control was developed by optimizing S. aureus-specific primers in combination with bovine specific primers. The effectiveness of the developed technique in this study was attempted in milk samples from bovine subclinical mastitis. This technique has the potential to detect S. aureus from bovine milk samples or dairy products.
