PCR ASSAY WITH HOST SPECIFIC INTERNAL CONTROL FOR STAPHYLOCOCCUS AUREUS FROM BOVINE MILK SAMPLES
Yükleniyor...
Dosyalar
Tarih
2015
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Univ Sv Kiril & Metodij Skopje, Fak Veterinarna Medicina
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Staphylococcus aureus is considered as one of the most important and common pathogens of bovine mastitis. Polymerase Chain Reaction is frequently proposed in the diagnosis of S. aureus directly from milk samples instead of classical culture. However, false-negative results may occur in the polymerase chain reaction analysis performed directly from clinical material. For the purpose of disclosing the false negative results, the use of internal amplification controls can be beneficial. Therefore, in this study a new polymerase chain reaction technique with host specific internal amplification control was developed by optimizing S. aureus-specific primers in combination with bovine specific primers. The effectiveness of the developed technique in this study was attempted in milk samples from bovine subclinical mastitis. This technique has the potential to detect S. aureus from bovine milk samples or dairy products.
Açıklama
Anahtar Kelimeler
bovine milk, internal control, polymerase chain reaction, Staphylococcus aureus
Kaynak
Macedonian Veterinary Review
WoS Q Değeri
N/A
Scopus Q Değeri
Q3
Cilt
38
Sayı
1
