PCR ASSAY WITH HOST SPECIFIC INTERNAL CONTROL FOR STAPHYLOCOCCUS AUREUS FROM BOVINE MILK SAMPLES

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dc.contributor.authorCantekin, Zafer
dc.contributor.authorErgun, Yasar
dc.contributor.authorSolmaz, Hasan
dc.contributor.authorOzmen, Gamze Ozge
dc.contributor.authorDemir, Melek
dc.contributor.authorSaidi, Radhwane
dc.date.accessioned2024-09-18T20:04:44Z
dc.date.available2024-09-18T20:04:44Z
dc.date.issued2015
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractStaphylococcus aureus is considered as one of the most important and common pathogens of bovine mastitis. Polymerase Chain Reaction is frequently proposed in the diagnosis of S. aureus directly from milk samples instead of classical culture. However, false-negative results may occur in the polymerase chain reaction analysis performed directly from clinical material. For the purpose of disclosing the false negative results, the use of internal amplification controls can be beneficial. Therefore, in this study a new polymerase chain reaction technique with host specific internal amplification control was developed by optimizing S. aureus-specific primers in combination with bovine specific primers. The effectiveness of the developed technique in this study was attempted in milk samples from bovine subclinical mastitis. This technique has the potential to detect S. aureus from bovine milk samples or dairy products.en_US
dc.identifier.doi10.14432/j.macvetrev.2015.01.038
dc.identifier.endpage100en_US
dc.identifier.issn1409-7621
dc.identifier.issn1857-7415
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-84924769337en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage97en_US
dc.identifier.urihttps://doi.org/10.14432/j.macvetrev.2015.01.038
dc.identifier.urihttps://hdl.handle.net/20.500.12483/8336
dc.identifier.volume38en_US
dc.identifier.wosWOS:000362207200012en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherUniv Sv Kiril & Metodij Skopje, Fak Veterinarna Medicinaen_US
dc.relation.ispartofMacedonian Veterinary Reviewen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectbovine milken_US
dc.subjectinternal controlen_US
dc.subjectpolymerase chain reactionen_US
dc.subjectStaphylococcus aureusen_US
dc.titlePCR ASSAY WITH HOST SPECIFIC INTERNAL CONTROL FOR STAPHYLOCOCCUS AUREUS FROM BOVINE MILK SAMPLESen_US
dc.typeArticleen_US

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