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  • Küçük Resim
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    The determination of Alpha Naphthyl Acetate Esterase (ANAE) activities in the peripheral blood leukocytes of geese (Anser anser)
    (Ecole Nationale Veterinaire Toulouse, 2009) Sari, E. Karadag; Kozlu, T.; Kocamis, H.; Deprem, T.; Asti, R. N.; Nazli, M.
    The objectives of this study were to investigate the presence of the Alpha Naphthyl Acetate Esterase (ANAE) in peripheral blood leukocytes in goose and to determine the proportions of blood T lymphocytes with this method. Blood smears obtained from 15 geese. 10 month old, were incubated at various pHs (5.0, 5.4, 5.8. 6.2, 6.4. 6.8 and 7.2 for 3 or 6 hours with the enzyme substrate. The highest resolution and consequently the best determination of positive ANAE cells were obtained with pH 5.8 for an incubation period of 6 hours. Positive ANAE activity wits only recorded in monocytes and in some lymphocytes (T cells) according, a diffuse and a granular pattern respectively whereas granulocytes, thrombocytes and B lymphocytes appeared ANAE negative. The mean ratio of ANAE positive lymphocytes peripheral blood ill goose was 53.6 +/- 3.2% and exhibited weak inter-individual fluctuations (coefficient of variations: 5.9%). These results suggest that ANAE staining method could be easily and efficiently used for T cell counting in peripheral blood.
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    Öğe
    The determination of Alpha Naphthyl Acetate Esterase (ANAE) activities in the peripheral blood leukocytes of geese {flnser anser)
    (2009) Sari, E. Karadag; Kozlu, T.; Kocamis, H.; Deprem, T.; Asti, R.N.; Nazli, M.
    The objectives of this study were to investigate the presence of the Alpha Naphthyl Acetate Esterase (ANAE) in peripheral blood leukocytes in goose and to determine the proportions of blood T lymphocytes with this method. Blood smears obtained from 15 geese, 10 month old, were incubated at various pHs (5.0, 5.4, 5.8, 6.2, 6.4, 6.8 and 7.2) for 3 or 6 hours with the enzyme substrate. The highest resolution and consequently the best determination of positive ANAE cells were obtained with pH 5.8 for an incubation period of 6 hours. Positive ANAE activity was only recorded in mono-cytes and in some lymphocytes (T cells) according a diffuse and a granular pattern respectively whereas granulocytes, thrombocytes and B lymphocytes appeared ANAE negative. The mean ratio of ANAE positive lymphocytes in peripheral blood in goose was 53.6 ± 3.2% and exhibited weak inter-individual fluctuations (coefficient of variations: 5.9%). These results suggest that ANAE staining method could be easily and efficiently used for T cell counting in peripheral blood.
  • Küçük Resim
    Öğe
    Immunohistochemical study on roe deer haemal nodes
    (Via Medica, 2018) Bozkurt, Y. Akaydin; Sari, E. Karadag; Kabak, M.
    Background: The aim of this study was to investigate the immunohistochemical characteristics of the haemal nodes located in the abdominal and thoracic cavities in roe deer (Capreolus capreolus). Materials and methods: In this study, 2 adult male and 2 adult female roe deers in addition to 2 roe deer foetuses at the late foetal stages were used. The avidin-biotin-peroxidase technique was applied to anti-CD3, anti-CD79 alpha cy, anti-macrophage, anti-S100 primary antibodies. Results: Positive results were gained for all the antibodies used. Many CD3 positive T-lymphocytes were seen in the lymphoid tissue areas of the foetal haemal nodes, in the germinal centre of the lymph follicles of the adult haemal nodes and in their lymphatic cords. CD79 alpha cy positive cells were less in number while S-100 protein was positive in both lymph follicles and in sinuses of the adult haemal nodes. In foetal haemal nodes, cells positive for S-100 protein were more prevalent in the sinuses. For the MAC387 primary antibody, there were positive macrophages seen in the capsule and trabeculae of the haemal nodes, located denser in males. Macrophage cells in the lymphoid structures and granulocytes in the sinuses of the haemal nodes of both adults and foetuses were positive for MAC387. Conclusions: This study is the first one to define the detailed structural features immunohistochemically in the haemal nodes of the roe deer. Even its concluded that the results were similar with the other ruminants, it's also pointed out that there are certain differences among the foetal, adult male and female haemal nodes of the roe deer