The determination of Alpha Naphthyl Acetate Esterase (ANAE) activities in the peripheral blood leukocytes of geese (Anser anser)
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Dosyalar
Tarih
2009
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Ecole Nationale Veterinaire Toulouse
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The objectives of this study were to investigate the presence of the Alpha Naphthyl Acetate Esterase (ANAE) in peripheral blood leukocytes in goose and to determine the proportions of blood T lymphocytes with this method. Blood smears obtained from 15 geese. 10 month old, were incubated at various pHs (5.0, 5.4, 5.8. 6.2, 6.4. 6.8 and 7.2 for 3 or 6 hours with the enzyme substrate. The highest resolution and consequently the best determination of positive ANAE cells were obtained with pH 5.8 for an incubation period of 6 hours. Positive ANAE activity wits only recorded in monocytes and in some lymphocytes (T cells) according, a diffuse and a granular pattern respectively whereas granulocytes, thrombocytes and B lymphocytes appeared ANAE negative. The mean ratio of ANAE positive lymphocytes peripheral blood ill goose was 53.6 +/- 3.2% and exhibited weak inter-individual fluctuations (coefficient of variations: 5.9%). These results suggest that ANAE staining method could be easily and efficiently used for T cell counting in peripheral blood.
Açıklama
Anahtar Kelimeler
ANAE, peripheral blood leukocytes, T lymphocytes, goose
Kaynak
Revue De Medecine Veterinaire
WoS Q DeÄŸeri
Q4
Scopus Q DeÄŸeri
Cilt
160
Sayı
4
