The determination of Alpha Naphthyl Acetate Esterase (ANAE) activities in the peripheral blood leukocytes of geese (Anser anser)

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dc.contributor.authorSari, E. Karadag
dc.contributor.authorKozlu, T.
dc.contributor.authorKocamis, H.
dc.contributor.authorDeprem, T.
dc.contributor.authorAsti, R. N.
dc.contributor.authorNazli, M.
dc.date.accessioned2024-09-18T19:54:17Z
dc.date.available2024-09-18T19:54:17Z
dc.date.issued2009
dc.departmentHatay Mustafa Kemal Ãœniversitesien_US
dc.description.abstractThe objectives of this study were to investigate the presence of the Alpha Naphthyl Acetate Esterase (ANAE) in peripheral blood leukocytes in goose and to determine the proportions of blood T lymphocytes with this method. Blood smears obtained from 15 geese. 10 month old, were incubated at various pHs (5.0, 5.4, 5.8. 6.2, 6.4. 6.8 and 7.2 for 3 or 6 hours with the enzyme substrate. The highest resolution and consequently the best determination of positive ANAE cells were obtained with pH 5.8 for an incubation period of 6 hours. Positive ANAE activity wits only recorded in monocytes and in some lymphocytes (T cells) according, a diffuse and a granular pattern respectively whereas granulocytes, thrombocytes and B lymphocytes appeared ANAE negative. The mean ratio of ANAE positive lymphocytes peripheral blood ill goose was 53.6 +/- 3.2% and exhibited weak inter-individual fluctuations (coefficient of variations: 5.9%). These results suggest that ANAE staining method could be easily and efficiently used for T cell counting in peripheral blood.en_US
dc.description.sponsorshipCommission for the Scientific Research Projects of Kafkas University [2007-VF-022]en_US
dc.description.sponsorshipThis project (project number: 2007-VF-022) was supported by the Commission for the Scientific Research Projects of Kafkas University.en_US
dc.identifier.endpage179en_US
dc.identifier.issn0035-1555
dc.identifier.issue4en_US
dc.identifier.startpage176en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12483/7634
dc.identifier.volume160en_US
dc.identifier.wosWOS:000265869200002en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherEcole Nationale Veterinaire Toulouseen_US
dc.relation.ispartofRevue De Medecine Veterinaireen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectANAEen_US
dc.subjectperipheral blood leukocytesen_US
dc.subjectT lymphocytesen_US
dc.subjectgooseen_US
dc.titleThe determination of Alpha Naphthyl Acetate Esterase (ANAE) activities in the peripheral blood leukocytes of geese (Anser anser)en_US
dc.typeArticleen_US

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