Yazar "Secer, Faik Sertel" seçeneğine göre listele

Listeleniyor 1 - 4 / 4
  • Küçük Resim
    Öğe
    Effect of different plant extracts on microbial population in the frozen African Catfish (Clarias gariepinus) semen
    (C M B Assoc, 2023) Secer, Faik Sertel; Yavas, Ilker; Cantekin, Zafer; Bozkurt, Yusuf; Yavas, Tugba Korkmaz; Atanasoff, Alexander
    Fish sperm cryopreservation has been attempted on roughly freshwater and marine species since 1953. This study sought to assess the potential of various plant extracts to function as natural antimicrobial agents in the frozen semen of African catfish (Clarias gariepinus). Diluted sperm was packaged in 0.25ml straws and left for 10min equilibration at 4 degrees C. Following equilibration, the straws were exposed to liquid nitrogen vapor for 10 min and plunged into the liquid nitrogen (-196 degrees C) and then thawed in a water bath at 35 degrees C for 20s. Sperm samples were put into sterile 1.5 ml tubes immediately after thawing and the microbial count was detected with classical microbiological culture method. In the results of microbiological analyses, these tree plant extracts especially Echinacea purpurea were found highly effective for decreasing bacterial contamination levels of African catfish (C. gariepinus) semen. These plant extracts may have the potential for antibacterial effect, and they can be useful for the dilution of semen.
  • [ N/A ]
    Öğe
    Effects of Seminal Plasma Composition on Sperm Motility in Mirror Carp (Cyprinus carpio)
    (Israeli Journal Of Aquaculture-Bamidgeh, 2009) Bozkurt, Yusuf; Ogretmen, Fatih; Secer, Faik Sertel; Ercin, Ugur
    This research provides data concerning the biochemical (ionic and organic) composition of sperm of the mirror carp (Cyprinus carpio) and its relationship to spermatozoa motility. Seminal plasma contained 67.12 +/- 1.06 mM/l Na+, 105.1 +/- 2.24 mM/l K+, 7.85 +/- 0.67 mg/dl Ca(2+), 2.61 +/- 0.11 mEq/l Mg(2+), 0.14 +/- 0.002 g/dl total protein, 10.3 +/- 1.01 mg/dl triglyceride, 6.83 +/- 0.72 mg/dl cholesterol, and 54.72 +/- 3.49 mg/dl urea. A positive relationship (p<0.05) was determined between Na(+) and motility (r = 0.522). On the other hand, Ca(2+), K(+), and Mg(2+) ions negatively correlated (p>0.05) with motility (r = -0.565, r = -0.160, and r = -0.184, respectively). Spermatozoa motility correlated negatively (p>0.05) with protein (r = -0.233), triglyceride (r = -0.348), and urea (r = -0.331) but positively with cholesterol (r = 0.012). This information will help to develop cryopreservation procedures, to meet species-specific extender requirements, and to optimize artificial fertilization procedures in mirror carp.
  • Küçük Resim
    Öğe
    Relationship Between Seminal Plasma Composition and Spermatological Parameters in Scaly Carp (Cyprinus carpio)
    (Medwell Online, 2009) Bozkurt, Yusuf; Ogretmen, Fatih; Secer, Faik Sertel; Ercin, Ugur
    The present study was conducted to evaluate seminal plasma composition, spermatological parameters and their relationships in scaly carp (Cyprinus carpio). Seminal plasma contained 71.18 +/- 3.40 mML(-1) (Na(+)), 87.48 +/- 4.42 mML(-1) (K(+)), 10.64 +/- 1.50 mg dL(-1) (Ca(2+)), 2.57 +/- 0.19 mEq L(-1) (Mg(2+)), 0.12 +/- 0.04 g dL(-1) total protein, 1.43 +/- 0.02 mg dL(-1) glucose, 7.82 +/- 2.60 mg dL(-1) triglyceride, 6.27 +/- 2.32 mg dL(-1) cholesterol, 24.45 +/- 7.98 mg dL(-1) urea. The following spermatological parameters were found: sperm volume 13.17 +/- 5.39 mL, sperm motility 63.18 +/- 7.16%, movement duration 56.81 +/- 20.32 s, sperm concentration 29.637 +/- 1.07 x 10(9) mL(-1), total sperm concentration 419.200 +/- 29.62 x 10(9) and pH 7.59 +/- 0.74. The K(+) and Ca(2+) ions correlated positively with motility (r = 0.215, p>0.05 and r = 0.048, p>0.05), respectively. On the other hand, Na(+) and Mg(2+) ions correlated negatively with motility (r = -0.067, p>0.05, r = -0.407, p>0.05), respectively. Significant correlations were observed between K(+) and Na(+) (r = -0.695, p<0.05), protein and triglyceride (r = 0.786, p<0.01), protein and cholesterol (r = 0.936, p<0.01), triglyceride and cholesterol (r = 0.802, p<0.01). These parameters can be used to prepare species-specific extenders for short-term storage and cryopreservation of scaly carp sperm.
  • Küçük Resim
    Öğe
    Short-Term Preservation of Grass Carp (Ctenopharyngodon idella) Eggs: Effect of Extenders and Storage Duration
    (Israeli Journal Of Aquaculture-Bamidgeh, 2010) Bozkurt, Yusuf; Ogretmen, Fatih; Secer, Faik Sertel
    The short-term effects of extenders and storage duration on fertilization, eyeing, and hatching rates in short-term stored grass carp eggs was evaluated. Eggs were kept in one of two artificial extenders (Ringer and 300 mM glucose solutions) or in ovarian fluid in 10-cm petri dishes. About 4 ml of artificial extender or coeolomic fluid was added to each petri dish and the dishes were transferred to a dark humid refrigerator for storage at 4 C for 120, 240, 360, or 480 min. Eggs were fertilized by adding 200 mu l milt to each petri dish. The fertilization rate was determined at the gastrula stage. The eyeing rate was defined as the number of eyed eggs divided by the initial number of eggs used for fertilization. The highest fertilization rate (93%) was attained in coelomic fluid stored for 120 min. The highest eyeing rates were in eggs kept in ovarian fluid for 120 or 240 min. The glucose extender was best for longer storage (360 and 480 min). The highest hatching rate was obtained with ovarian fluid at 240 min but the glucose extender was best for storage at 120, 360, and 480 min. Results indicate that glucose solution is the best extender for short-term (2-4 h) storage of grass carp eggs.