Yazar "Sertkaya, G" seçeneğine göre listele

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    Characterization of Plum pox potyvirus (PPV) by DAS-ELISA and RT-PCR/RFLP analysis in Turkey
    (Int Soc Horticultural Science, 2004) Caglayan, K; Sertkaya, G; Ulubas, C; Kölber, M; Krizbai, L
    This study was conducted to determine the presence of Plum pox virus (PPV) (family Potyviridae, genus Potyvirus) in different regions of Turkey and to characterise PPV isolates by serological and molecular techniques including ELISA, PCR/ RFLP. Thus, leaf samples from different stone fruit species, almond, apricot, nectarine, peach, plum, sour and sweet cherry exhibiting various types of symptoms related to PPV were collected from different parts of the canopy from randomly selected orchards in the main stone fruit growing areas in Turkey. Polyclonal antibodies (PAbs) were used to detect the presence of PPV in the plant samples by serological assays (DAS-ELISA). The following monoclonal antibodies (Mabs): Mab5B (Universal), Mab4DG5 (PPV-D: Dideron-specific), MabAL (PPV-M: Marcus-specific), MabEA24 (PPV-El Amar-specific) and MabAC (PPV-C: Cherry-specific) were used to identify the serotyping of PPV isolates. Reverse transcription-polymerase chain reaction (RT-PCR) assays and restriction fragment length polymorphism (RFLP) analysis of RT-PCR products were performed to characterize Turkish PPV isolates. The results of RT-PCR analyses using general primers were in complete agreement with the DAS-ELISA and DASI-ELISA results, showing that 2 apricot samples of 52 stone fruit samples collected from Ankara province were found to be infected with M strain of PPV. This study confirmed the results of the previous work demonstrated the presence of PPV-M strain on apricot in Turkey.
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    Detection and characterization of phytoplasmas in diseased stone fruits and pear by PCR-RFLP analysis in Turkey
    (Springer, 2005) Sertkaya, G; Martini, M; Ermacora, P; Musetti, R; Osler, R
    During the late summer-early autumn of 2002, surveys were carried out in Turkey to determine the presence of phytoplasma diseases in fruit trees. Phytoplasmas were detected and characterized by PCR-RFLP analysis and TEM technique in stone fruit and pear trees in the eastern Mediterranean region of the country. Six out of 24 samples, including almond, apricot, peach, pear and plum, gave positive results in PCR assays. RFLP analysis using SspI and BsaAI enzymes of PCR products obtained with primer pair f01/r01 enabled identification of the phytoplasmas involved in the diseases. Stone fruit trees, including a local apricot variety ('Sakit') and a pear sample, were found to be infected with European stone fruit yellows (ESFY, 16SrX-B) and pear decline (PD, 16SrX-C) phytoplasmas, respectively. This is the first report in Turkey of PD phytoplasma infecting pear and of ESFY phytoplasma. infecting almond, apricot, myrobalan plum and peach; ESFY phytoplasma infecting Japanese plum was previously reported.
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    Detection of phytoplasmas in Rubus spp. by microscopy and molecular techniques in Turkey
    (Int Soc Horticultural Science, 2004) Sertkaya, G; Osler, R; Musetti, R; Ermacora, P; Martini, M
    Samples (shoots and leaves) were collected in August 2002 from symptomatic plants in two cultivated blackberry (Rubus spp.) fields and from wild blackberry (Rubus fruticosus L.) plants growing at the border of citrus orchards in Adana, Hatay and Icel provinces in Eastern Mediterranean Region of Turkey. Blackberry plants were observed to show symptoms consisting of numerous short, weak, thin and erect shoots arising from single buds. These shoots produce minute leaves and confer upon the plants a bushy appearance. Cuttings of symptomatic plants were rooted in pots and kept in insect-proof growing room. Small bits of stems taken from symptomatic plants (2 cultivated and 7 wild blackberries) were stained with DAPI and examined with an epifluorescence microscope. Two DAPI-positive samples were also prepared for electron microscopy. Ultra-thin sections of midribs were then examined under a Philips CM10 transmission electron microscope (TEM). After DAPI staining, typical fluorescence was detected in sieve tubes of all symptomatic plants. In ultra-thin sections, phytoplasma bodies of different sizes and shapes occurred in the sieve tubes of midribs. According to symptoms observation of diseased plants, to DAPI staining and TEM analyses, it is suggested that the causal agent of the disease in Rubus spp. is a phytoplasma. The results obtained from PCR/RFLP analyses confirmed the presence of phytoplasmas in Rubus samples. This is the first report of rubus stunt disease in Turkey. Further studies are necessary to investigate distribution and natural transmission of the disease in cultivated Rubus spp.
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    Detection of some viruses of stone fruits in mother plant blocks in eastern Mediterranean region of Turkey
    (Int Soc Horticultural Science, 2004) Sertkaya, G; Caglayan, K; Ulubas, C
    Field inspection and sample collection were carried out in mother plant blocks which are also varietal collection belonging to the Ministry of Agriculture and the Universities in Eastern Mediterranean Region of Turkey during early spring and in autumn in 2002. Samples (shoots and leaves) were collected from symptomatic plants in the orchards. 48 samples (3 almond, 9 apricot, 3 nectarine, 10 peach, It plum, 12 sweet cherry) having different symptoms were collected and tested for the presence of Apple chlorotic leaf spot trichovirus (ACLSV), Apple mosaic ilarvirus (ApMV), Cherry leaf roll nepovirus (CLRV), Prune dwarf ilarvirus (PDV), Prunus necrotic ringspot ilarvirus (PNRSV) and Plum pox potyvirus (PPV) by using DAS-ELISA. According to serological assays, the most common virus was PDV. It was followed by PNRSV and ACLSV. The reverse transcription-polymerase chain reaction (RT-PCR) assays were performed for investigation of PDV, PNRSV and PPV in the samples. The following viruses were identified by ELISA and RT-PCR assays: ACLSV (1 sample), CLRV (1 sample), PDV (5 samples), PNRSV (2 samples). Mix infections of ACLSV+PDV (1 sample) and PDV+PNRSV (2 samples) were also found. No PPV-infected samples were detected from the samples collected from mother plant blocks in Eastern Mediterranean Region of Turkey.
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    Incidence and insect transmission of tomato yellow leaf curl virus in Hatay province of Turkey
    (International Society Horticultural Science, 2005) Sertkaya, G; Sertkaya, E
    Tomato yellow leaf curl virus (TYLCV) is one of the most common viruses infecting tomatoes in the Mediterranean region. Tomato samples with typical viral symptoms were collected from tomatoes grown in the open field and greenhouses in 2002-2003 in Hatay province. These samples were tested for TYLCV, CMV, PVX, PVY and PLRV by DAS-ELISA. TYLCV was detected in 18-49% and 13-32% of the field-collected samples for the different districts for 2002-2003, respectively. For the samples collected in the greenhouse, TYLCV infection rates varied from 10-27% and 6-16 for the Samandag district in 2002-2003, respectively. Tomatoes that tested positive for TYLCV exhibited severe stunting, chlorotic leaf edges, upward leaf cupping, and reduced leaf size. TYLCV-infected tomatoes were observed in tomato transplants in January, when mature tomato plants and whiteflies were present. Bemisia tabaci adults collected from natural infected tomatoes were able to transmit TYLCV to tomato seedlings. Growers should adopt integrated pest management strategies for control of TYLCV infections and should evaluate current moderately resistant hybrids.
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    Obtaining cucumber mosaic virus (CMV)-free production material by meristem tip and nodal segment culture in sweet potato (Ipomoea batatas (L.) Lam.)
    (Diagnosis Press Ltd, 2002) Sertkaya, G
    This study was performed to survey CMV infection in sweet potato grown in South Turkey and to obtain CMV-free plants by meristem tip culture and to multiply that material by nodal segment culture. Sweet potato grown areas (Serinyol and Iskenderun) in Hatay were surveyed during June-August 2001 and 2002 and the plants with symptoms were searched for presence of cucumber mosaic virus (CMV) by using biological indexing and ELISA methods. The results of the survey conducted at two locations (Serinyol and iskenderun) showed that percentage of the plants with stunting, symptom ranged between 4.5% and 6% and percentage of the plants with common chlorosis and yellowing symptoms between 15% and 12.4% as depending on the locations. Regeneration ratio of the meristem tips from CW-infected plants of sweet potato genotype Hatay beyazi was 33.3%. Totally 36 plantlets were propagated from 4 CMV-free plantlets in 6 weeks after meristem tip culture stage by nodal segment culture and 35 plantlets of them began to develop10 days after transfer into pots.