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    INVESTIGATION OF THE ANTIBIOTIC RESISTANCE AND BIOFILM FORMATION OF STAPHYLOCOCCUS AUREUS STRAINS ISOLATED FROM GANGRENOUS MASTITIS OF EWES
    (Akademiai Kiado Zrt, 2012) Tel, Osman Yasar; Aslantas, Ozkan; Keskin, Oktay; Yilmaz, Ebru Sebnem; Demir, Cemil
    In this study, Staphylococcus aureus strains (n = 110) isolated from seven ewe flocks in Sanliurfa, Turkey were screened for antibiotic resistance and biofilm-forming ability as well as for genes associated with antibiotic resistance and biofilm-forming ability. All isolates were found to be susceptible to oxacillin, gentamicin, clindamycin, cefoxitin, tetracycline, vancomycin, amoxicillin-clavulanic acid, ciprofloxacin and sulphamethoxazole-trimethoprim. The percent proportions of strains resistant to penicillin G, ampicillin and erythromycin were 27.2% (n = 30), 25.4% (n = 28) and 6.3% (n = 7), respectively. Regarding the antibiotic resistance genes, 32 (29%) isolates carried the blaZ and 8 (7.2%) the ermC gene. Other resistance genes were not detected in the isolates. All isolates showed biofilm-forming ability on Congo red agar (CRA), while 108 (98.18%) and 101 (91.81%) of them were identified as biofilm producers by the use of standard tube (ST) and microplate (MP) methods, respectively. All isolates carried the icaA and icaD genes but none of them harboured the bap gene. The results demonstrated that S. aureus isolates from gangrenous mastitis were mainly resistant to penicillins (which are susceptible to the staphylococcal beta-lactamase enzyme), and less frequently to erythromycin. Furthermore, all of the S. aureus isolates produced biofilm which was considered a potential virulence factor in the pathogenesis of staphylococcal mastitis.
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    Investigation of Toxin Genes of Staphylococcus aureus Strains Isolated from Gangrenous Mastitis in Ewes
    (Kafkas Univ, Veteriner Fakultesi Dergisi, 2011) Tel, Osman Yasar; Aslantas, Ozkan; Keskin, Oktay; Yilmaz, Ebru Sebnem; Demir, Cemil
    In this study, it was aimed to determine staphylococcal enterotoxin (SE) genes, toxic shock syndrome toxin (TSST) gene and exfoliative toxin (ET) genes in 110 Staphylococcus aureus strains from gangrenous mastitis cases in seven ewe flocks in Sanliurfa, Turkey. Among investigated isolates, only sec and tst toxin genes were detected. The results of this study showed that the rates of enterotoxin production were high (100%) in S. aureus strains isolated from ovine gangrenous mastitis. Moreover, it was demonstrated that S. aureus causing gangrenous mastitis harboured sec and tst genes suggesting that these genes may have a role in ovine mastitis pathogenesis.
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    Pathological and Microbiological Investigations of Pneumonic Pasteurellosis in Sheep
    (Kafkas Univ, Veteriner Fakultesi Dergisi, 2013) Ozyildiz, Zafer; Tel, Osman Yasar; Yilmaz, Rahsan; Ozsoy, Sule Yurdagul; Keskin, Oktay
    In this study, between March 2010 and March 2011, 110 pneumonia suspected lung tissues were examined histopathologically, immunohistochemically and microbiologically, in Sanliurfa province. After definition of the macroscopic localization of the consolidated areas in the lungs, tissue samples were taken and preserved in cold chain and 10% formalin for microbiological and pathological examinations, respectively. For bacteriological examination of Pasteurella spp. lung specimens were inoculated in 7% sheep blood agar and Mc Conkey agar. After routine pathological tissue follow up procedure, tissues were embedded in paraffin and obtained sections stained with Hematoxylin&Eosin (H&E). The cases, having histopathological findings consistent with pneumonia, were forwarded to immunohistochemical (IHC) examinations to know whether lesions related to Mannheimia haemolytica and Pasteurella multocida using hyperimmune polyclonal rabbit sera in Avidin Biotin Complex Peroxidase (ABC-P). Microbiological, histopathological and immunohistochemical findings were comparatively evaluated in examined animals. Pasteurella multocida as a cause of pneumonia were detected in 38 cases of microbiological inoculations. Immunohistochemical staining resulted Mannheimia haemolytica (n=35) and Pasteurella multocida (n=30) positive. Immunohistochemically both Mannheimia haemolytica and Pasteurella multocida were positive in 23 cases and 45 animals were negative for both bacteria. The aim of this study is to show importance and role of Pasteurella spp, in sheep pneumonia in Sanliurfa region.