Yazar "Terzi, M. Y." seçeneğine göre listele

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    Effect of Glucose Starvation on Cell Viability Parameters in A549 and BEAS-2B Cell Lines
    (Pleiades Publishing Inc, 2022) Cetiner, B. G.; Terzi, M. Y.
    Cancer cells are metabolically altered cells characterized by higher glucose uptake. Understanding cancer cells is vital in cancer therapy. We aimed to analyze glucose starvation effect on cell viability parameters in healthy (BEAS-2B) and cancer (A549) cells. We analyzed mTOR, p21, caspase-3, beclin-1, ATG-12 mRNA levels in cells after 8 and 24 h starvations. We also counted senescent cells post-starvation. Starvation decreased cell viability in both cell types whereas prolonged starvation kept reducing cell viability just in A549 cells. Along with elevated p21 mRNA levels, we detected more senescent cells in A549 than in BEAS-2B after starvation. Increased mTOR expression supported senescence profile in cancer cells at 8 h starvation unlike healthy cells. Autophagy marker expression increased after-starvation in both cell lines except reduction in BEAS-2B cells after 8 h starvation. Caspase-3 expression did not change in BEAS-2B cells while increased in A549 cells at 8 h starvation. As to 24 h starvation, caspase-3 was upregulated in both cells, however, it was higher in cancer cells compared to healthy cells. We suggest that cell viability parameters of healthy and cancer cells were negatively affected by prolonging glucose starvation. Our results demonstrated that glucose starvation had more negative effects on cancer cells than healthy cells.
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    Reduced Expression of PEDF and ALDH1A1 during Spheroid Transition of Lung Cancer Cells: An In Vitro Study
    (Pleiades Publishing Inc, 2022) Terzi, M. Y.; Okuyan, H. M.; Gulbol-Duran, G.; Urhan-Kucuk, M.
    Cancer stem cells (CSCs) can initiate tumorigenesis and metastasis and show resistance against chemotherapy owing to the expression of prominent stem cell markers. CSCs are a subpopulation of highly heterogenic cancer spheroid cells. Pigment epithelium-derived factor (PEDF) is a neurotrophic, anti-tumorigenic, and anti-metastatic protein and its gene expression levels in A549 spheroids is still unknown. We aimed to compare clonogenicity and mRNA levels of PEDF, Oct4, and ALDH1A1 between A549 and spheroid cells. Spheroid and colony formation assays were performed with spheroid and A549 cells. We performed quantitative reverse transcription-polymerase chain reaction (qRT-PCR) for gene expression analysis. The clonogenic ratios for A549 and spheroids were similar to 60% and similar to 1% respectively. During spheroid formation, Oct4 mRNA level did not change but PEDF and ALDH1A1 levels decreased significantly. CSCs are characterized by elevated stem cell markers but spheroid cells consist of heterogeneous population including CSCs. In spheroid population, no increase in stem cell markers was observed. The reduced PEDF levels during spheroid transition can be a suppression mechanism of spheroid cells.