Effect of Glucose Starvation on Cell Viability Parameters in A549 and BEAS-2B Cell Lines
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Dosyalar
Tarih
2022
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Pleiades Publishing Inc
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Cancer cells are metabolically altered cells characterized by higher glucose uptake. Understanding cancer cells is vital in cancer therapy. We aimed to analyze glucose starvation effect on cell viability parameters in healthy (BEAS-2B) and cancer (A549) cells. We analyzed mTOR, p21, caspase-3, beclin-1, ATG-12 mRNA levels in cells after 8 and 24 h starvations. We also counted senescent cells post-starvation. Starvation decreased cell viability in both cell types whereas prolonged starvation kept reducing cell viability just in A549 cells. Along with elevated p21 mRNA levels, we detected more senescent cells in A549 than in BEAS-2B after starvation. Increased mTOR expression supported senescence profile in cancer cells at 8 h starvation unlike healthy cells. Autophagy marker expression increased after-starvation in both cell lines except reduction in BEAS-2B cells after 8 h starvation. Caspase-3 expression did not change in BEAS-2B cells while increased in A549 cells at 8 h starvation. As to 24 h starvation, caspase-3 was upregulated in both cells, however, it was higher in cancer cells compared to healthy cells. We suggest that cell viability parameters of healthy and cancer cells were negatively affected by prolonging glucose starvation. Our results demonstrated that glucose starvation had more negative effects on cancer cells than healthy cells.
Açıklama
Anahtar Kelimeler
Keywords, apoptosis, autophagy, cancer cell, cell viability, glucose starvation, senescence
Kaynak
Cytology and Genetics
WoS Q Değeri
Q4
Scopus Q Değeri
Q3
Cilt
56
Sayı
1
