Effect of Glucose Starvation on Cell Viability Parameters in A549 and BEAS-2B Cell Lines

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dc.authoridTerzi, Menderes Yusuf/0000-0001-8478-0451
dc.contributor.authorCetiner, B. G.
dc.contributor.authorTerzi, M. Y.
dc.date.accessioned2024-09-18T20:28:16Z
dc.date.available2024-09-18T20:28:16Z
dc.date.issued2022
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractCancer cells are metabolically altered cells characterized by higher glucose uptake. Understanding cancer cells is vital in cancer therapy. We aimed to analyze glucose starvation effect on cell viability parameters in healthy (BEAS-2B) and cancer (A549) cells. We analyzed mTOR, p21, caspase-3, beclin-1, ATG-12 mRNA levels in cells after 8 and 24 h starvations. We also counted senescent cells post-starvation. Starvation decreased cell viability in both cell types whereas prolonged starvation kept reducing cell viability just in A549 cells. Along with elevated p21 mRNA levels, we detected more senescent cells in A549 than in BEAS-2B after starvation. Increased mTOR expression supported senescence profile in cancer cells at 8 h starvation unlike healthy cells. Autophagy marker expression increased after-starvation in both cell lines except reduction in BEAS-2B cells after 8 h starvation. Caspase-3 expression did not change in BEAS-2B cells while increased in A549 cells at 8 h starvation. As to 24 h starvation, caspase-3 was upregulated in both cells, however, it was higher in cancer cells compared to healthy cells. We suggest that cell viability parameters of healthy and cancer cells were negatively affected by prolonging glucose starvation. Our results demonstrated that glucose starvation had more negative effects on cancer cells than healthy cells.en_US
dc.description.sponsorshipScientific Research Project Funds of Hatay Mustafa Kemal University [19]en_US
dc.description.sponsorshipThis study was supported by the Scientific Research Project Funds of Hatay Mustafa Kemal University (Project no. 19.YL.007).en_US
dc.identifier.doi10.3103/S0095452722010054
dc.identifier.endpage76en_US
dc.identifier.issn0095-4527
dc.identifier.issn1934-9440
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-85124964608en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage66en_US
dc.identifier.urihttps://doi.org/10.3103/S0095452722010054
dc.identifier.urihttps://hdl.handle.net/20.500.12483/10816
dc.identifier.volume56en_US
dc.identifier.wosWOS:000754205100008en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherPleiades Publishing Incen_US
dc.relation.ispartofCytology and Geneticsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectKeywordsen_US
dc.subjectapoptosisen_US
dc.subjectautophagyen_US
dc.subjectcancer cellen_US
dc.subjectcell viabilityen_US
dc.subjectglucose starvationen_US
dc.subjectsenescenceen_US
dc.titleEffect of Glucose Starvation on Cell Viability Parameters in A549 and BEAS-2B Cell Linesen_US
dc.typeArticleen_US

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