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Öğe Bazı Fidanlıklarda Görülen Toprak Kökenli Fungusların Belirlenmesi(2021) Uysal, Aysun; Kurt, Şener; Kara, Merve; Soylu, Soner; Soylu, Emine MineGaziantep Orman Fidanlık Şefliği, Osmaniye Orman Fidanlık Şefliği, Hatay Serinyol Orman Fidanlık Şefliği ve HMKÜ kampüs fidanlık alanında 14 farklı peyzaj bitkisi türünden toplam 98 adet hastalıklı fidan örneği toplanmıştır. Bu örneklerin 81’inde, toprak kökenli hastalık etmenleri saptanmıştır. Bu etmenlerin tanılanmasına yönelik olarak morfolojik sonuçlar değerlendirildiğinde; farklı konukçulardan elde edilen hastalık etmenlerinden 12 adedi, Ceratobasidium sp, 8’i Fusarium, 14’ü Macrophomina, 13’ü Rhizoctonia, 4’ü Phytophthora ve 10’u Dactylonectria olarak saptanmış; geriye kalan 20 tanesi de Phoma, Pestalotiopsis, Diaporthe, Didymella ve Neopestalotiopsis fungusları olarak belirlenmiştir. Moleküler çalışmalar kapsamında ITS1-4 primeri ile yapılan PCR çalışmaları ve DNA dizileme sonuçlarına göre tür düzeyinde tanıları yapılmıştır. Temsili seçilen 16 izolatın, NCBI GenBankası kütüphanesinden erişim numaraları alınmıştır. Sonuç olarak, fidan sağlığını gözeterek fidanlıklarda her türlü mücadele yöntemlerinin bir arada etkili ve sürdürülebilir şekilde uygulanması, peyzaj sahalarının korunmasında önemli bir yer tutacaktır.Öğe Ceviz gövde kanseri hastalığı etmeni Botryosphaeria dothidea’nın tanılanması ve bazı fungisitlerin hastalık etmenine karşı in vitro antifungal etkinliklerinin belirlenmesi(Mustafa Kemal Üniversitesi, 2020) Kurt, Şener; Soylu, Soner; Uysal, Aysun; Soylu, Emine Mine; Kara, MerveAmaç: Çalışmada Hatay’ın İskenderun ilçesinde Mayıs 2019 tarihinde ceviz bahçelerdeki ceviz ağaçlarında, iletim dokularında kahverengi-koyu kahverengi renk değişikliği, odun dokusu nekrozu ve dallarda geriye doğru ölüm hastalık belirtilerine neden olan fungal etmenin tanılanması ve etmenin bazı fungisitlere karşı duyarlılık düzeylerinin belirlenmesi amaçlanmıştır. Yöntem ve Bulgular: Hastalıklı dallardan, kanserlerden ve belirti göstermeyen ceviz örneklerinden yapılan izolasyonlar sonucunda elde edilen fungal izolat morfolojik ve moleküler çalışmalara göre Botryosphaeria dothidea olarak tanılanmıştır. Patojenisite denemeleri, fungal izolatın bölgede yetiştirilen Chandler çeşidi ceviz ağacı sürgünlerinde oldukça virülent olduğunu göstermiştir. Mevcut bilgilerimize göre bu çalışma, Türkiye’de cevizi enfekte eden Botryosphaeria dothidea’nın ilk raporudur. Fluazinam (0.001-0.015 µg/ml), thiophanate-methyl (0.1-10.0 µg/ml), boscalid + pyraclostrobin (1.0-50.0 µg/ml), trifloxystrobin (0.5-50.0 µg/ml), kresoxim-methyl (0.1-50.0 µg/ml) ve tebuconazole (0.5-50.0 µg/ml) gibi 6 farklı fungisitin farklı konsantrasyonlarının, fungal etmene karşı in vitro antifungal etkileri belirlenmiştir. Fungisitlerin ortalama etkili konsantrasyon (EC50) değerleri, Probit analiziyle belirlenmiştir. Hesaplanan EC50 değerlerine göre hastalık etmenine karşı en etkili fungisit fluazinam (0.002 µg/ml) olarak kaydedilmiş olup, bu fungisiti thiophanate-methyl (0.275 µg/ml), tebuconazole (0.994 µg/ml), boscalid + pyraclostrobin (3.993 µg/ml) ve trifloxystrobin (4.262 µg/ml) izlemiştir. En düşük antifungal etkinlik fungisitler arasında misel gelişimini %37.5 oranında engelleyen kresoxim-methyl tarafından gösterilmiştir. Genel Yorum: Bu çalışma fungal etmen Botryosphaeria dothidea’nın Türkiye’de yetiştirilen ceviz ağaçlarında gövde kanseri hastalığa neden olduğunun ilk kayıdıdır. Elde edilen sonuçlar ayrıca Botryosphaeria dothidea’nın sebep olduğu ceviz kanser hastalığının seçilen fungisitlerin uygulanmasıyla ağaçların hastalıktan korunabileceğini önermektedir. Çalışmanın Önemi ve Etkisi: Sonuç olarak, fluazinam, thiophanate-methyl ve tebuconazole’ün fungal patojen B. dothidea’nın kontrolünde kullanım potansiyeli bulunduğunu ortaya koymaktadır.Öğe Characterization and pathogenicity of Fusarium solani associated with dry root rot of citrus in the eastern Mediterranean region of Turkey(Springer Japan Kk, 2020) Kurt, Sener; Uysal, Aysun; Soylu, E. Mine; Kara, Merve; Soylu, SonerFusarium dry root rot is one of the most dangerous diseases of citrus in Turkey. Since December 2015, a devastating decline has caused light purple, vascular discolorations, and dry decay of fibrous roots in some citrus orchards. Cream-colored colonies produced ovoid, ellipsoid or reniform, 1-2-celled microconidia. Macroconidia were 3-5 septate, hyaline, straight, or slightly curved. Chlamydospores were produced from mycelium in intercalary or terminal chains. The fungus was identified as Fusarium solani by sequencing of the ITS and tef-1 alpha nucleotides, pathogenicity assay and MALDI-TOF MS. In pathogenicity tests on 'Okitsu', 20 isolates of Fusarium spp. caused typical root rot.Öğe Colletotrichum gloeosporiodes causing anthracnose on pomegranate in Turkey(Springer, 2018) Uysal, Aysun; Kurt, SenerDuring spring 2017, an anthracnose-like foliar disease was observed in the pomegranate orchards in the region. Disease incidence (%) ranged from 15 to 22. The objectives of this study were to characterise this fungal pathogen, based on morphology, molecular characteristics and pathogenicity. Foliar symptoms progressed light to dark brown, concentric, circular, distinct spots with yellowish halos. Also, salmon coloured spore masses were observed on pomegranate cv. Hicaz leaves in Kozan district. Cultural and morphological examinations and DNA sequence data revealed that the fungus associated with symptomatic leaves was Colletotrichum gloeosporioides. Pathogenicity testing was performed by inoculation of healthy leaves with a spore suspension of C. gloeosporioides. This confirmed C. gloeosporioides as causal agent of anthracnose symptoms on pomegranate in Turkey.Öğe Control of Monilinia blossom and twig blight (Monilinia laxa) by boron, pyroligneous acid and boscalid(Springer, 2024) Uysal, AysunMonilinia laxa is one of the most destructive fungal pathogens causing brown rot, blossom blight and spur infection on stone fruits such as peach, nectarine, apricot, cherry and plum. In 2019-2020 surveys, 30 orchards in the apricot and almond production areas of Malatya and Elaz & imath;g provinces of Turkey exhibited blossom and twig blight symptoms. The objective of this study was to evaluate the in vitro and in vivo efficacy of boron products, pyroligneous acid and some fungicides on Monilinia laxa. Boron derivatives and boscalid were tested at different concentrations (0.01, 0.03, 0.05, 0.07, 0.09, 0.1,0.2, 0.4, 0.6%; w/v, mu g.ml(-1)) for their inhibitory effect on mycelial growth of M. laxa. Mycelial growth of M. laxa was completely inhibited by boric acid (0.1%), borax (0.09%), Etidot (0.09%), pyroligneous acid (0.8%) and boscalid (0.6 mu gml(-1)). Boric acid (0.12%), borax (0.1%), Etidot-67 (0.15%), PA (1.2%) and boscalid (0.8 mu g ml(-1)) significantly inhibited germination of conidia of M. laxa isolates. EC50 values for 4 isolates of M. laxa ranged from 0.028 to 0.569% (MIC values ranged from 0.09 to 0.8 mu gml(-1)) for mycelial growth and from 0.052 to 0.750% (MIC values ranged from 0.1 to 0.12) for conidial germination. In field trials conducted in the two subsequent years, boscalid + pyraclostrobin showed the highest efficacy (82.4% and 85.2%), followed by PA + borax (64.2% and 65.6%), PA + boric acid (48.2% and 54.6%), and boric acid + borax (18.2% and 21.9%, respectively).Öğe Determination of antagonistic potential of endophytic bacteria isolated from lettuce against lettuce white mould disease caused by Sclerotinia sclerotiorum(Lithuanian Research Centre Agriculture & Forestry, 2021) Soylu, Soner; Kara, Merve; Uysal, Aysun; Kurt, Sener; Soylu, Emine MineIn this study, biocontrol efficiency of endophytic bacterial isolates obtained from the leaf lettuce (Lactuca sativa L.) healthy plants was investigated against white mould disease agent Sclerotinia sclerotiorum in vitro and in vivo conditions. Antagonistic efficiency of endophytic bacterial isolates was determined to inhibit mycelial growth and sclerotial germination, suppress disease incidence caused by S. sclerotiorum. A total of 48 endophytic bacterial isolates were isolated from different tissues of lettuce healthy plants. Mycelial growth and germination of sclerotia of S. sclerotiorum in vitro inhibited 18 bacterial isolates. Bacillus subtilis and B. amyloliquefaciens isolates were found to be the most efficient ones, which significantly inhibited the mycelial growth by 68.1-83.1%, germination of sclerotia by 82.7-89.6% and suppressed disease incidence by 55.7-75% caused by S. sclerotiorum. In addition, the fungal mycelium close to the inhibition zone in dual culture was denser and darker in colour. B. subtilis and B. amyloliquefaciens isolates caused significant morphological alterations in hyphae such as hyphal shrivelling and perforation close to the inhibition zone in dual culture. Significant suppression in the mycelial growth, sclerotial germination and disease incidence caused by endophytic bacterial isolates indicate that isolates of B. subtilis and B. amyloliquefaciens could be considered as possible biocontrol agents against soil-borne fungal diseases.Öğe Determination of Biocontrol Potentials of Endophytic Bacteria in Biological Control of Citrus Sour Rot Disease Caused by Geotrichum citri-aurantii(Univ Namik Kemal, 2022) Soylu, Soner; Kara, Merve; Soylu, Emine Mine; Uysal, Aysun; Kurt, SenerCitrus fruits are susceptible to postharvest decays caused by various pathogenic fungal disease agents. Citrus sour rot, caused by Geotrichum cirri-aurantii is one of the most important postharvest diseases of citrus fruits Although synthetic fungicides are commonly used to combat the disease, ineffectiveness of these applications as well as their harmful effects on human health and the environment have been reported in many studies recently. Biological control of postharvest diseases utilizing antagonistic bacteria has been explored in different host plant-pathogen interactions as a promising alternative to synthetic fungicides. In this study, biocontrol potentials of endophytic bacterial isolates, obtained from fruits, branch and leaves of healthy citrus trees, were investigated against Geotrichum citri-aurantii on petri plates (in vitro) and fruit tests (semi in vivo conditions). Among the 24 putative endophytic bacterial isolates isolated, sixteen different antagonist bacterial isolates were selected and assessed against mycelial growth inhibition of G. citri-aurantii in vitro studies. Among the bacterial isolates tested, Bacillus spp. isolates significantly inhibited mycelial growth of fungal agent by 59.5-78.6%. The highest mycelial growth inhibition was caused by B. subtilis CM8 (78.6%). Bacterial isolates of Acinetobacter johnsonii CT7, Erwinia herbicola CM5, Pseudomonas putida CL2, Pantoea agglomerans CM14, Acinetobacter lwoffii CP1 and Stenofrophomonas maltophilia CP3 failed to inhibit mycelial growth in dual culture assays. Antagonistic activities of bacterial isolates increased by the pre-incubation time before fungal inoculation. Bacterial isolates of Bacillus spp. caused considerably morphological changes such as vacuolation, shrivelling, swelling and lysis of hyphae close to inhibition zone. On artificially inoculated fruits, isolates of Bacillus spp. also found to suppressed disease incidence significantly by 56.7-83.3%. Due to strong antagonistic activities, isolates of Bacillus spp, tested in this study have the potential to be used as biofungicide for controlling post-harvest disease agent(s) of citrus fruits.Öğe Determination of Fungal and Bacterial Disease Agents on Significant Brassicaceous Vegetable Species Grown in Hatay Province(Kahramanmaras Sutcu Imam Univ Rektorlugu, 2024) Soylu, Soner; Kara, Merve; Uysal, Aysun; Gumus, Yusuf; Soylu, Emine Mine; Kurt, Sener; Uremis, IlhanThe Brassicaceae family, commonly known as the Cruciferae or mustard family, encompasses plant species of global economic significance, including oilseed crops, vegetables, as well as condiment purposes. Hatay is one of the most important provinces in Turkey where Brassicaceous vegetable crops are grown. This study aimed to identify the causative disease agents affecting Brassicaceous vegetables including cabbage (red and white varieties), broccoli, cauliflower, garden cress, rocket, and radish in the districts of Hatay Province where vegetable cultivation took place during the 2020-2021 growing seasons. Isolations from suspicious cabbage, broccoli, cauliflower, and radish plants exhibiting disease symptoms in the surveyed areas revealed the presence of various fungal disease agents, including Rhizoctonia solani, Sclerotinia sclerotiorum, Fusarium solani, Fusarium oxysporum, Fusarium exquisite, Alternaria alternata, Botrytis cinerea, , and Stemphylum sp. Furthermore, obligate oomycete pathogens such as Albugo lepidii, Albugo candida, , and Perofascia lepidii were detected with varying prevalence and incidence rates on leaves and stems of water cress, rocket, and radish plants. In addition to fungal pathogens, primary bacterial pathogens, including Xanthomonas campestris pv. campestris, , Pectobacterium carotovorum subsp. carotovorum, , Pectobacterium parmentieri, Pseudomonas corrugata, , and Pseudomonas victoria, , were identified through morphological, biochemical, and pathogenicity tests, and MALDI-TOF analyses. Pantoea agglomerans, Pseudomonas marginalis, Pseudomonas fluorescens, Enterobacter cloacae, and Bacillus pumilus were also identified as opportunistic soft rot bacterial pathogens. To the best of our knowledge, this is the first report of F. solani, F. oxysporum, F. exquisite, A. alternata, B. cinerea, Stemphylum sp., as fungal disease agents; P. parmentieri, P. corrugata, and P. victoria as primary soft rot bacterial disease agents; P. agglomerans, P. marginalis, P. fluorescens, E. cloacae, and B. pumilus as opportunistic secondary soft rot bacterial disease agents affecting different minor vegetables belong to Brassica spp, such as cabbage (red and white varieties), broccoli, cauliflower, radish, rocket, and garden cress, grown in Turkey.Öğe Determination of Fungal and Bacterial Diseases Agents Encountered in Carrot Growing Fields in Amik Plain of Turkey(Kahramanmaras Sutcu Imam Univ Rektorlugu, 2022) Soylu, Soner; Kara, Merve; Uysal, Aysun; Kurt, Sener; Soylu, Emine Mine; Uremis, Ilhan; Sertkaya, ErdalCarrot [Daucus carota L.] is one of the most consumed healthy vegetables worldwide due to its vitamins, amino acids and high nutritional content. Fungal and bacterial disease agents, encountered in the fields where carrots are grown in the Amik Plain of Hatay province, were determined during 2018-2019 growing season. Disease surveys were carried out at three different phenological stages of the plant. Different fungal and bacterial disease agents were isolated and subsequently identified from diseased leaves, stems and fruits. In addition to the disease symptoms in the form of transverse brown dry depressions on carrot fruits, from the brown-black spots on the leaves and stems, Alternaria dauci, A. alternata, Rhizoctonia solani, R. carotae, Botrytis cinerea, Sclerotinia sclerotiorum, Geotrichum candidum, Pythium spp., Fusarium bra. equiseti, F. proliferatum and F. solani species were obtained and identified as a result of molecular and MALDI-TOF protein profile analyzes as well as their morphological features. Powdery mildew caused by Erysiphe heraclei was also determined in the surveyed area. By the bacterial isolates obtained from carrot showing soft rot symptoms, major soft rot disease agents, such as Pectobacterium carotovorum subsp. carotovorum and Pseudomonas corrugata and opportunistic soft rot disease agents, such as P. marginalis, P. fluorescens, P. mediterranea, P. veronii, Erwinia rhapontici, E. persicina, Enterobacter cloacae, Lelliottia amnigena, Rahnella aquatilis, Pantoea agglomerans, were identified by using MALDI-TOF analyses. To the best of our knowledge, this is the first report in which fungal disease agent Fusarium brachygibbosum and all soft rotting bacterial disease agents were determined at species level on carrot roots growing in Turkey.Öğe Determination of Soil-Borne Fungi Observed in Some Nurseries(Centenary University, 2021) Uysal, Aysun; Kurt, Şener; Kara, Merve; Soylu, Soner; Soylu, Emine MineA total of 98 diseased seedlings of 14 different landscape plants were collected from Gaziantep forest nursery chief, Osmaniye forest nursery chief, Hatay Serinyol forest nursery chief and HMKU campus nursery. Soil-borne disease factors were identified in 81 of these samples. When morphological results are evaluated for the identification of these agents; disease agents from different hosts were identified as 12 of Ceratobacidium sp, 8 of Fusarium, 14 of Macrophomina, 13 of Rhizoctonia, 4 of Phytophthora, 10 of Dactylonectria, and the remaining 20 of Phoma, Pestalotiopsis, Diaporthe, Didymella, and Neopestalotiopsis. Within the scope of molecular studies, PCR studies were conducted with ITS1-4 primer and according to DNA sequencing results species level diagnoses were made. Representative of 16 isolates accession numbers are obtained from NCBI Gen bank library. Consequently, effective and sustainable implementation of all kinds of combat methods in nurseries, taking into consideration the health of the saplings, will have an important place in the protection of landscape areas. © 2021, Centenary University. All rights reserved.Öğe Determination of the Chemical Composition and Antifungal Activity of Wood Vinegar (Pyroligneous Acid) Against the Onion Bulb Rot Disease Caused by Fusarium proliferatum(Springer Science and Business Media Deutschland GmbH, 2023) Kara, Merve; Soylu, Soner; Soylu, E. Mine; Uysal, Aysun; Kurt, Şener; Türkmen, MusaWood vinegar, also known as pyroligneous acid (PA), is a liquid mixture with organic properties that results from the condensation of smoke produced by the charring or pyrolysis of wood and its residues during processing. The aims of this study were to determine the chemical composition and antifungal activity of three different PAs obtained from the pyrolysis of apricot kernels (AKPA), hazelnut shells (HSPA), and kermes oak (OPA) against Fusarium proliferatum. As a result of GC-MS analysis, o?guaiacol (18.98–26.18%), creosol (11.08–16.38%), syringol (6.35–8.67%), p?ethylguaiacol (5.53–10.04%), o?creosol (4.0–10.33%) and phenol (2.34–7.90%) were identified as the main constituents of the tested PAs. AKPA was the most effective PA among the tested treatments, with MIC values of 3.5?µl ml?1 for mycelial growth and 1.0?µl ml?1 for conidial germination. Of the treatments tested under in vivo conditions, AKPA completely inhibited disease incidence at a concentration of 7.5?µl g?1 soil. HSPA and OPA, on the other hand, significantly suppressed disease incidence at concentrations of 10.0?µl g?1and 12.5?µl g?1 soil, respectively. All PA samples with MIC values caused significant deterioration of the morphological structures of the fungal hyphae. Since PAs showed promising antifungal activities against F. proliferatum both in vitro and in vivo, they could be used as a cost-effective natural product as an environmentally friendly alternative to synthetic fungicides. © 2023, The Author(s), under exclusive licence to Springer-Verlag GmbH Deutschland, part of Springer Nature.Öğe Distribution and characterization of Colletotrichum species associated with Citrus anthracnose in eastern Mediterranean region of Turkey(Springer, 2022) Uysal, Aysun; Kurt, Sener; Guarnaccia, VladimiroCitrus are economically important fruit crops produced worldwide. Anthracnose, post bloom fruit drop, and stem-end rot of fruit caused by Colletotrichum species are diseases that seriously threatens citrus production in Turkey. Surveys conducted in 2016-2017 allowed the detection of typical symptoms of anthracnose of the visited fields (126 among 203) in the citrus production area of the Eastern Mediterranean region of Turkey. A total of 194 Colletotrichum strains were obtained from different citrus cultivars, including lemon (85), mandarin (73), orange (30), sour orange (1) and grapefruit (5) and different plant tissues (twigs (90), leaves (84) and fruits (20)). A multi-locus phylogeny was established based on four genomic loci (ITS, GAPDH, ACT, CHS-1), and the morphological characters of the isolates were determined. Based on morphological characters and phylogenetic analysis, isolates were identified as belonging to C. gloeosporioides and C. karsti. Pathogenicity trials showed that, both species were able to colonise leaves, twigs, and fruits of the susceptible cultivar Interdonato of Citrus limon. Symptoms that developed on inoculated tissues were similar to those observed in the field on naturally infected citrus plants. Pathogenicity trials showed that C. gloeosporioides was more virulent on twigs and leaves, and C. karsti on fruits. To the best of our knowledge, this is the most comprehensive study on identification and characterization of C. gloeosporioides and C. karsti associated with citrus anthracnose in Turkey.Öğe Doğu Akdeniz Bölgesinde turunçgillerde antraknoz etmeni colletotrichum türlerinin morfolojik, patojenik, moleküler karakterizasyonu ve fungisit duyarlılıkları(Hatay Mustafa Kemal Üniversitesi, 2019) Uysal, Aysun; Kurt, ŞenerAntraknoz, dünyada ve ülkemizde turunçgil üretimini ciddi düzeyde tehdit eden hastalıklardan birisidir. Turunçgillerde antraknoz hastalığına Colletotrichum cinsine ait türler neden olmaktadır. Bu çalışmada, Doğu Akdeniz Bölgesi'nde turunçgil üretim alanlarında antraknoz etmeni Colletotrichum türlerini belirlemek için 2016-2017 yılları arasında sörveyler gerçekleştirilmiştir. Bölgede yapılan sörveylerde yoğun bir şekilde antraknoz belirtileri gözlenmiştir. Antraknoz hastalığının Doğu Akdeniz Bölgesi'nde yaygınlığının %62,1 oranında olduğu saptanmıştır. Sörveyler sonucunda toplanan örneklerden 161 adet Colletotrichum izolatı elde edilmiştir. Toplanan bu izolatlardan 72'si limon, 40'ı mandarin, 13'ü portakal, biri altıntop ve biri de turunçtan elde edilmiştir. Morfolojik çalışmalar sonucunda; koloni rengi, konidi şekil ve boyutları ile eşeyli üreme yapıları, apresoryum yapıları gibi morfolojik tanı karakterlerine göre izolatlar, C. gloeosporioides ve C. karstii olarak tanımlanmıştır. ITS, GAPDH, ACT, CHS VE TUB gen bölgelerine göre multilokus filogenetik dizi analizleri gerçekleştirilmiştir. Elde edilen bulgulara göre limonda C. gloeosporioides'in 40 ve C. karstii'nin 34 izolatı kaydedilmiştir. Mandarinde ise C. gloeosporioides ve C. karstii türlerine ait, sırasıyla 32 ve 8 izolat saptanmıştır. Bununla birlikte C. gloeosporioides türüne ait olarak portakalda 13, altıntopda 1 ve turunçta 1 izolat belirlenmiştir. Dört genin birlikte kullanılarak kombinasyon halinde oluşturulan dendrogramda; izolatlar C. gloeosporioides ve C. karstii şeklinde iki gruba ayrılmıştır. MALDI TOF MS analiz sonucunda, C. gloeosporioides türünde 63 izolat; C. karstii türünde ise; 30 izolat 2.000-2.299 (yeşil renk) arası değer alarak güvenilir düzeyinde tür tanısı yapılmıştır. C. gloeosporioides türünde 25 izolat; C. karstii türünde 12 izolat 1.700-1.999 (sarı renk) arası değer ile muhtemel cins düzeyinde tanımlama yapılmıştır. Colletotrichum gloeosporioides, C. gloeosporioides tür kompleksinde, Colletotrichum karstii ise C. boninense tür kompleksinin bir üyesi olarak olarak belirlenmiştir. Patojenisite denemelerinde; C.gloeosporioides türünde LC5 izolatı, C. karstii türünde ise LCk36 izolatının yüksek düzeyde virülent oldukları belirlenmiştir. Fungisit duyarlılık çalışmaları miselyal gelişim ve konidiyal çimlenme denemeleri şeklinde iki aşamalı olarak yapılmıştır. Fungisit denemeleri sonucunda, en etkili olarak myclobutanil, trifloxystrobin, boscalid+pyraclostrobin, carbendazim+ chlorothalonil ve thiophanate-methyl fungusitleri belirlenmiştir. Söz konusu çalışma, bildiğimiz kadarı ile, ülkemizde turunçgil antraknoz hastalığı ile ilişkili Colletotrichum türlerinin tanılanması ve mücadelesi konusunda yapılan en geniş kapsamlı çalışmadır. Çalışma sonucunda elde edilen veriler hem bilimsel olarak hemde turunçgil üreticileri açısından oldukça önem taşımaktadır. Bu çalışma ile, yıllardır turunçgillerde göz ardı edilen antraknoz hastalığı üreticiler tarafından tam olarak tanınmadığı ortaya çıkmıştır. Üreticiler tarafından uçkurutan (Phoma tracheiphila) olarak bilinen hastalığın artık geri planda kaldığı ve yerini antraknoz (Colletotrichum spp.) hastalığına bıraktığı yapılan arazi gözlemleri ve laboratuvar analizleri ile kesinlik kazanmıştır.Öğe First report of bacterial shallow bark canker of walnut (Juglans regia) caused by Brenneria nigrifluens in Turkey(Springer, 2021) Soylu, Soner; Kara, Merve; Bozkurt, Imam Adem; Soylu, Emine Mine; Kurt, Sener; Uysal, Aysun[Abstract Not Available]Öğe First report of Colletotrichum karstii causing anthracnose on citrus in the Mediterranean region of Turkey(Springer, 2019) Uysal, Aysun; Kurt, Sener[Abstract Not Available]Öğe First report of Colletotrichum siamense causing anthracnose on banana fruits in Turkey(Springer, 2020) Uysal, Aysun; Kurt, Sener[Abstract Not Available]Öğe First report of Diplodia seriata causing canker and dieback on hawthorn trees in Turkey(Springer, 2022) Kurt, Sener; Uysal, Aysun; Soylu, Emine Mine; Kara, Merve; Soylu, Soner[Abstract Not Available]Öğe First report of fruit and leaf anthracnose caused by Colletotrichum karstii on avocado in Turkey(Elsevier Sci Ltd, 2020) Uysal, Aysun; Kurt, SenerAvocado (Persea americana Mill.) is one of the most popular deciduous fruit crops grown in tropical and subtropical areas worldwide. Colletotrichum spp., causal agents of anthracnose, are the species commonly associated with fruit and leaf symptoms on avocado in Turkey and all over the world. Since early 2016, necrotic symptoms were detected on avocado trees grown in the Mediterranean Region of Turkey. Fungal isolates obtained from the systematic tissues were examined based on mycelial growth rate, colony colour, conidial shapes and dimensions. Morphological characteristics of the representative isolates AC1 and AC2 of Colletotrichum were similar to those described for C. karstii and the isolates were tentatively identified as Colletotrichum spp. In view of its colonies and conidia features. Morphological data were verified by multilocus phylogenetic analyses of the internal transcribed spacer (ITS) region of the nuclear ribosomal DNA; glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and ACTIN partial genes from two isolates. Pathogenicity tests were conducted by inoculating healthy fruit and leaves with a spore suspension of C. karstii. Unlike the species previously recorded in Turkey, this fungus was first reported as avocado anthracnose pathogen with this study.Öğe First report of Neofusicoccum parvum causing branch dieback on Juglans regia in Turkey(Springer, 2021) Kara, Merve; Soylu, Emine Mine; Soylu, Soner; Uysal, Aysun; Kurt, Sener[Abstract Not Available]Öğe First report of powdery mildew caused by Erysiphe sedi on Kalanchoe blossfeldiana in Turkey(Springer, 2021) Soylu, Soner; Kurt, Sener; Kara, Merve; Uysal, Aysun; Soylu, Emine Mine; Choi, In-Young[Abstract Not Available]
