Yazar "Yonar, M. Enis" seçeneğine göre listele

Listeleniyor 1 - 6 / 6
  • Küçük Resim
    Öğe
    Ameliorative effect of lycopene on antioxidant status in Cyprinus carpio during pyrethroid deltamethrin exposure
    (Academic Press Inc Elsevier Science, 2011) Yonar, M. Enis; Sakin, Fatih
    The aim of the present study was to investigate the ameliorative properties of lycopene against the toxic effects of deltamethrin (DM) by examining oxidative damage markers such as lipid peroxidation and the antioxidant defense system components in carp (Cyprinus carpio). The fish were divided into seven groups of 15 fish each and received the following treatments: Group 1, no treatment; Group 2, orally administered corn oil; Group 3, oral lycopene (10 mg/kg body weight); Group 4, exposure to 0.018 mu g/L DM; Group 5, exposure to 0.018 mu 4/L DM plus oral administration of 10 mg/kg lycopene; Group 6, exposure to 0.036 mu g/L DM; and Group 7, exposure to 0.036 mu g/L. DM plus oral administration of 10 mg/kg lycopene. Treatment was continued for 14 days, and at the end of this period, blood and tissue (liver, kidney, and gill) samples were collected. Levels of malondialdehyde (MDA) and reduced glutathione (GSH) as well as superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities were determined in blood and tissues for measurement of oxidant-antioxidant status. A significant elevation in the level of MDA, as an index of lipid peroxidation, and reductions in antioxidant enzyme activities (SOD, CAT, and GSH-Px) and low molecular weight antioxidant (GSH) levels were observed in DM-exposed fish. Treatment with lycopene attenuated the DM-induced oxidative stress by significantly decreasing the levels of MDA. In addition, lycopene significantly increased the SOD, CAT, and GSH-Px activities and the level of GSH. The present results suggest that administration of lycopene might alleviate DM-induced oxidative stress. (C) 2011 Elsevier Inc. All rights reserved.
  • Küçük Resim
    Öğe
    Changes in Selected Immunological Parameters and Oxidative Stress Responses in Different Organs of Oncorhynchus mykiss Exposed to Ivermectin
    (Revista Chimie Srl, 2012) Sakin, Fatih; Yonar, Serpil Mise; Yonar, M. Enis; Saglam, Naim
    In this study, effects of ivermectin on selected immunological parameters, oxidative stress, and antioxidant status in the blood, liver, kidney, heart, and spleen of the rainbow trout (Oncorhynchus mykiss) were examined. Ivermectin was administered intraperitoneally at single doses of 0.01 and 0.02 mg ivermectin kg(-1) fish. Blood and tissue samples (liver, kidney, heart, and spleen) were then removed on days 3, 7, 10, and 14 for immunological and biochemical analyses. The immunological parameters measured were numbers of leucocytes (WBC), nitroblue tetrazolium activity (NBT), total plasma protein (TP), and total immunoglobulin (TI). Malondialdehyde (MDA) values were measured in the liver, kidney, heart, and spleen tissues as indexes of oxidation, and the activities of catalase (CAT), reduced glutathione (GSH), glutathione peroxidase (GSH-Px), and superoxide dismutase (SOD) were measured as indexes of antioxidant status. The results showed a decrease in the WBC value, NBT activity, and TP and TI levels after the injection of 0.01 and 0.02 mg ivermectin kg(-1) fish (p <0.05, 0.01, 0.001). Our results suggest that ivermectin at these doses causes oxidation in fish liver, kidney, heart, and spleen tissues due to impairment of the antioxidant system.
  • [ N/A ]
    Öğe
    EFFECT OF SHORT-TERM CYPERMETHRIN EXPOSURE ON OXIDANT-ANTIOXIDANT BALANCE IN THE WHOLE BODY OF RAINBOW TROUT FRY (Oncorhynchus mykiss)
    (Parlar Scientific Publications (P S P), 2011) Sakin, Fatih; Ispir, Unal; Yonar, Serpil Mise; Yonar, M. Enis; Taysi, M. Resit
    The objective of this study was to investigate the propensity of cypermethrin (CYP) to induce whole body oxidative stress in rainbow trout following exposure. We investigated the effect of CYP (10%, 5%, 2% and 1% of the 4-day rainbow trout now-through LC50 1.46 mu g/L CYP) on antioxidants in rainbow trout fry, Oncorhynchus mykiss, using standard laboratory conditions. 24 h exposure caused induction of malondialdehyde (MDA) and various antioxidant enzymes such as catalase (CAT), glutathione peroxidase (GSH-Px) and reduced glutathione (GSH) in the whole body. Fish were monitored for clinical signs. Results obtained showed that CYP significantly (p < 0.05) increased MDA level. Compared to controls, activities of GSH, GSH-Px, and CAT were significantly (p < 0.05) reduced in the whole bodies of rainbow trout fry exposed to CYP.
  • Küçük Resim
    Öğe
    Effect of trichlorfon on some haematological and biochemical changes in Cyprinus carpio: The ameliorative effect of lycopene
    (Elsevier, 2020) Yonar, Serpil Mise; Yonar, M. Enis; Pala, Aysegul; Saglam, Naim; Sakin, Fatih
    Trichlorfon (TCF) is an antiparasitic agent used in aquaculture to treat external parasites and it belongs to the chemical class of organophosphates. This study was conducted to investigate the ameliorating effects of lycopene on TCF-induced toxicity in carp by evaluating haematological parameters and oxidant/antioxidant status. Fish were randomly divided into seven experimental groups. These groups were arranged as follows: control (no treatment) group, corn oil group, lycopene (10 mg kg(-1)) group, TCF-I (11 mg L-1) group, TCF-I (11 mg L-1) plus lycopene (10 mg kg(-1)) group, TCF-II (22 mg L-1) group and TCF-II (22 mg L-1) plus lycopene (10 mg kg(-1)) group. At the end of 14 days administration, blood and tissue (liver, kidney, and gill) samples were collected. Haematological changes [red blood cell (RBC) and white blood cell (WBC) count, haemoglobin (Hb) concentration, haematocrit (Ht) level, and erythrocyte indices: mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), and mean corpuscular haemoglobin concentration (MCHC)] were determined in the blood samples, while antioxidant parameters [(malondialdehyde (MDA) and reduced glutathione (GSH) levels and superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities] were evaluated in the liver, kidney and gill samples. The results demonstrated that all the haematological parameters were significantly decreased in the TCF-I and TCF-II groups when compared to the control group. Notable increases in the tissue MDA levels were observed in the TCF-treated groups. In contrast, significant decreases were observed in the tissue SOD, CAT and GSH-Px activities and GSH levels in the TCF-I and TCF-II groups. However, simultaneous treatment with lycopene provided a marked normalisation of the haematological and oxidant/antioxidant parameters compared with the TCF-I and TCF-II groups. The findings show that dietary lycopene supplementation might be helpful in abrogation of TCF toxicity by changing haematological conditions and antioxidant status in fish.
  • [ N/A ]
    Öğe
    OXIDATIVE STRESS BIOMARKERS OF EXPOSURE TO DELTAMETHRIN IN RAINBOW TROUT FRY (Oncorhynchus mykiss)
    (Parlar Scientific Publications (P S P), 2011) Yonar, Serpil Mise; Sakin, Fatih; Yonar, M. Enis; Ispir, Unal; Kirici, Muammer
    Deltamethrin (DM) is a widely used pesticide based on pyrethroids, and is reported to be extremely toxic to fish species. We investigated the effect of DM concentrations (0.010, 0.0125 and 0.0250 mu g/L) on antioxidants in rainbow trout, Oncorhynchus mykiss, using standard laboratory conditions. 24 h exposure caused whole body induction of various antioxidant enzymes and nonenzymatic antioxidants. Results showed that DM significantly (p<0.05) increased malondialdehyde (MDA) levels. Catalase (CAT) activity decreased in all experimental groups. Glutathione (GSH) was significantly (p<0.05) increased in the group with DM concentration of 0.0250 mu g/L DM for 24 h. The activity of glutathione peroxidase (GSH-Px) was found to be statistical significantly (p<0.05) decreased in all other experimental groups except the 0.010 mu g/L DM treated group. The parameters studied in this investigation can also be used as biomarkers of exposure to DM. It is suggested that appropriate ecotoxicological risk assessments should be made wherever DM is proposed for use in pest control activities.
  • Küçük Resim
    Öğe
    Oxidative stress biomarkers of exposure to deltamethrin in rainbow trout fry (oncorhynchus mykiss)
    (2011) Yonar, Serpil Mişe; Sakin, Fatih; Yonar, M. Enis; Ispir, Ünal; Kinci, Muammer
    Deltamethrin (DM) is a widely used pesticide based on pyrethroids, and is reported to be extremely toxic to fish species. We investigated the effect of DM concentrations (0.010, 0.0125 and 0.0250 ?g/L) on antioxidants in rainbow trout, Oncorhynchus mykiss, using standard laboratory conditions. 24 h exposure caused whole body induction of various antioxidant enzymes and nonenzymatic antioxidants. Results showed that DM significantly (p< 0.05) increased malondialdehyde (MDA) levels. Catalase (CAT) activity decreased in all experimental groups. Glutathione (GSH) was significantly (p<0.05) increased in the group with DM concentration of 0.0250 ?g/L DM for 24 h. The activity of glutathione peroxidase (GSH-Px) was found to be statistical significantly (p<0.05) decreased in all other experimental groups except the 0.010 ?g/L DM treated group. The parameters studied in this investigation can also be used as biomarkers of exposure to DM. It is suggested that appropriate ecotoxicological risk assessments should be made wherever DM is proposed for use in pest control activities. © by PSP.