Uterin leiomyom ve endometrial polip etyopatogenezinde TGF- β1'in rolü
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Tarih
2022
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Hatay Mustafa Kemal Üniversitesi
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info:eu-repo/semantics/openAccess
Özet
Amaç: Bu çalışmada endometrial poliplerin ve uterin leiomiyomların patogenezinde Transforming Growth Factor Beta 1 (TGF-β1)'in rolünü değerlendirmeyi ve TGF-β1 inhibitörleri gibi immünomodülasyonun, cerrahi tedavi yerine hedefe yönelik tedavide faydalı olabileceğini göstermeyi amaçladık. Gereç ve Yöntem: Çalışmamız retrospektif vaka kontrol tipi bir çalışmadır. Çalışmamıza uterin leiomyom tanısı alan 30 ve endometrial polip tanısı alan 30 olgu dahil edildi. Kontrol grubu olarak normal myometrium ile normal endometrium dokusuna sahip 30 olgu dahil edildi. Hastaların demografik ve klinik özelliklerine ait veriler hastane veri tabanı sistemi üzerinden tarandı ve kaydedildi. İmmünohistokimyasal çalışmalar için doku kesitleri ksilol ile deparafinize, alkol ile dehidrate edildi. TGF-β1 antikorları ile 1/200 dilüsyonda 30 dakika inkübasyon süresince DAKO Autostainer Link 48 cihazı ile immünohistokimyasal boyama yapıldı. Hematoksilen ile zıt boyama uygulandı. Endometrial polip, leiomyom ve normal dokudaki TGF-β1 düzeyleri semi-kantitatif yöntem ile değerlendirildi. İstatistiksel anlamlılık p<0,05 kabul edildi. Bulgular: Çalışmaya dahil edilen 90 bireyin yaş ortalaması 45,03±5,23 yıl olarak bulundu. Gruplar arasında yaş ortalamaları benzerdi (p=0,097). Gravida ve parite değerleri açısından gruplar arasında anlamlı bir fark saptanmadı (sırasıyla p=0,252, p=0,064). Vücut kitle indeksi açısından gruplar arasında anlamlı bir fark saptanmadı (p=0,457). Leiomyom dokularında normal myometrium dokusuna kıyasla TGF-β1 boyanma yoğunluğu daha yüksek bulundu (p=0,004). Leiomyom dokularında normal myometrium dokusuna kıyasla TGF-β1 boyanma yüzdesi daha yüksek bulundu (p=0,045). Endometrial polip dokularında normal endometrium dokularına kıyasla TGF-β1 boyanma yoğunluğu ve yüzdesi daha yüksek bulundu (p=0,029) ( P=0,019). Sonuç: Çalışmamızda leiomyom ve endometrial polip dokularında TGF- β1 ekspresyonunun kontrol myometrium ve endometrium dokularına kıyasla daha yüksek olduğunu bulduk. Sonuçlarımızın doğrulanması ve cerrahiye alternatif hedefe yönelik tedavi için geniş kohortta yapılacak çalışmalara ihtiyaç vardır.
Aims: In this study, we aimed to evaluate the role of Transforming Growth Factor Beta 1 (TGF-β1) in the pathogenesis of endometrial polyps and uterine leiomyomas and to show that immunomodulation such as TGF-β1 inhibitors may be beneficial in targeted therapy instead of surgical therapy. Materials and Methods: This study is a retropective case-control type study. Thirty individuals diagnosed with uterine leiomyoma and 30 individuals diagnosed with endometrial polyps were included in our study. As the control group, 30 individuals with normal myometrium and normal endometrium tissue were included. The demographic and clinical characteristics of the patients were scanned and recorded through the hospital database system. Tissue sections were deparaffinized with xylol and dehydrated with alcohol for immunohistochemical studies. Immunohistochemical staining was performed with TGF-β1 antibodies at 1/200 dilution during 30 minutes of incubation with DAKO Autostainer Link 48 device. Counterstaining with hematoxylin was applied. TGF-β1 levels in endometrial polyp, leiomyoma and normal tissue were evaluated by semi-quantitative method. Statistical significance was accepted as p<0,05. Results: The mean age of 90 individuals included in the study was 45,03±5,23 years. The mean age was similar between the groups (p=0,097). There was no significant difference between the groups in terms of gravida and parity values (p=0,252, p=0,064, respectively). There was no significant difference between the groups in terms of body mass index (p=0,457). TGF-β1 staining intensity was found to be higher in leiomyoma tissues compared to normal myometrium tissue (p=0,004). TGF-β1 staining intensity was found to be higher in leiomyoma tissues compared to normal myometrium tissue (p=0,045). TGF-β1 staining intensity was found to be higher in endometrial polyp tissues compared to normal endometrial tissues (p=0,029). Conclusion: In our study, we found that TGF-β1 expression was higher in leiomyoma and endometrial polyp tissues compared to control myometrium and endometrial tissues. There is a need for further studies in a large cohort to confirm our results and to provide alternative targeted therapy to surgery.
Aims: In this study, we aimed to evaluate the role of Transforming Growth Factor Beta 1 (TGF-β1) in the pathogenesis of endometrial polyps and uterine leiomyomas and to show that immunomodulation such as TGF-β1 inhibitors may be beneficial in targeted therapy instead of surgical therapy. Materials and Methods: This study is a retropective case-control type study. Thirty individuals diagnosed with uterine leiomyoma and 30 individuals diagnosed with endometrial polyps were included in our study. As the control group, 30 individuals with normal myometrium and normal endometrium tissue were included. The demographic and clinical characteristics of the patients were scanned and recorded through the hospital database system. Tissue sections were deparaffinized with xylol and dehydrated with alcohol for immunohistochemical studies. Immunohistochemical staining was performed with TGF-β1 antibodies at 1/200 dilution during 30 minutes of incubation with DAKO Autostainer Link 48 device. Counterstaining with hematoxylin was applied. TGF-β1 levels in endometrial polyp, leiomyoma and normal tissue were evaluated by semi-quantitative method. Statistical significance was accepted as p<0,05. Results: The mean age of 90 individuals included in the study was 45,03±5,23 years. The mean age was similar between the groups (p=0,097). There was no significant difference between the groups in terms of gravida and parity values (p=0,252, p=0,064, respectively). There was no significant difference between the groups in terms of body mass index (p=0,457). TGF-β1 staining intensity was found to be higher in leiomyoma tissues compared to normal myometrium tissue (p=0,004). TGF-β1 staining intensity was found to be higher in leiomyoma tissues compared to normal myometrium tissue (p=0,045). TGF-β1 staining intensity was found to be higher in endometrial polyp tissues compared to normal endometrial tissues (p=0,029). Conclusion: In our study, we found that TGF-β1 expression was higher in leiomyoma and endometrial polyp tissues compared to control myometrium and endometrial tissues. There is a need for further studies in a large cohort to confirm our results and to provide alternative targeted therapy to surgery.
Açıklama
Anahtar Kelimeler
Kadın Hastalıkları ve Doğum, Obstetrics and Gynecology, Uterin leiomyom, endometrial polip, TGF-β1, Uterine leiomyoma, endometrial polyp, TGF-β1
