The Usefulness of Serum Brain Damage Biomarkers in Detection and Evaluation of Hypoxic Ischemic Encephalopathy in Calves with Perinatal Asphyxia
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Dosyalar
Tarih
2022
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Mdpi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Simple Summary The objective of the present study was to determine hypoxic brain damage in calves with perinatal asphyxia using brain-specific damage biomarkers. Ten healthy calves and 25 calves with perinatal asphyxia were enrolled in the study. Consciousness evaluation and laboratory analyses were performed at admission, 24, 48, and 72 h. Serum concentrations of brain-related biomarkers were measured to assess brain injury. Moreover, histopathological and immunohistochemical examinations of the brain tissue were performed in 13 nonsurvivor calves. The consciousness level of the calves with asphyxia was significantly lower than the healthy calves. Mix metabolic-respiratory acidosis and hypoxemia were detected in calves with asphyxia. Serum UCHL1 and S100B concentrations were significantly increased, and NSE, ACTA, ADM, and CK-B were decreased in calves with asphyxia. Histopathological and immunohistochemical examination in nonsurvivor calves confirmed the development of mild to severe hypoxic-ischemic encephalopathy. In conclusion, asphyxia causes hypoxic ischemic encephalopathy in perinatal calves. UCHL1 and S100B were found to be useful markers of hypoxic-ischemic encephalopathy in calves with perinatal asphyxia. Neurological status scores and some blood gas parameters were helpful in mortality prediction. The purpose of the present study was to determine hypoxic brain damage in calves with perinatal asphyxia using brain-specific damage biomarkers. Ten healthy and 25 calves with perinatal asphyxia were enrolled in the study. Clinical examination, neurological status score, and laboratory analysis were performed at admission, 24, 48, and 72 h. Serum concentrations of ubiquitin carboxy-terminal hydrolysis 1 (UCHL1), calcium-binding protein B (S100B), adrenomodullin (ADM), activitin A (ACTA), neuron-specific enolase (NSE), glial fibrillary acidic protein (GFAP) and creatine kinase-brain (CK-B) were measured. Histopathological and immunohistochemical examinations of the brain tissue were performed in 13 nonsurvivor calves. The neurological status score of the calves with asphyxia was significantly (p < 0.05) lower. Mix metabolic-respiratory acidosis and hypoxemia were detected in calves with asphyxia. Serum UCHL1 and S100B were significantly (p < 0.05) increased, and NSE, ACTA, ADM, and CK-B were decreased (p < 0.05) in calves with asphyxia. Histopathological and immunohistochemical examinations confirmed the development of mild to severe hypoxic-ischemic encephalopathy. In conclusion, asphyxia and hypoxemia caused hypoxic-ischemic encephalopathy in perinatal calves. UCHL1 and S100B concentrations were found to be useful markers for the determination of hypoxic-ischemic encephalopathy in calves with perinatal asphyxia. Neurological status scores and some blood gas parameters were helpful in mortality prediction.
Açıklama
Anahtar Kelimeler
perinatal asphyxia, calf, brain damage, serum biomarkers, mortality
Kaynak
Animals
WoS Q DeÄŸeri
Q1
Scopus Q DeÄŸeri
Q1
Cilt
12
Sayı
22
