Siyatik sinir hasarı oluşturulan ratlarda elektriksel stimülasyonun etkilerinin araştırılması
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Tarih
2021
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Hatay Mustafa Kemal Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Sunulan çalışmada, siyatik sinir hasarı oluşturulan rat modelinde, elektriksel stimülasyonun sinir sistemi üzerine etkilerinin araştırılması amaçlandı. Çalışmada 28 adet Wistar Albino rat (2 aylık, erkek, 250-300 g) kullanıldı. Her bir grupta 7 hayvan olup, 4 grup oluşturuldu; kontrol (K) grubu, elektriksel stimülasyon (ES) grubu, siyatik sinir hasarı (SSH) grubu ve siyatik sinir hasarı + elektriksel stimülasyon (SSH+ES) grubu. Deneysel siyatik sinir hasarı modeli, siyatik sinir 50 g/cm'lik bir kapanma kuvveti olan Yasargil FE 693 geçici anevrizma ile 2 dakika boyunca sıkıştırılarak yapıldı. Elektriksel stimülasyon 200 μs akım süresinde, 20 Hz frekansta, 2mA amplitude ile 20 dk 15 gün boyunca uygulandı. Çalışmanın sonunda tüm ratlar sakrifiye edildi. Siyatik sinir hasarı oluşturulan ratlarda elektriksel stimülasyonun sinirler üzerine olan etkileri, serum ve beyin dokusu beyin kaynaklı nörotrofik faktör (BDNF) ve sinir büyüme faktörü (NGF) düzeylerinın belirlenmesi, ELİSA analizleri ile ortaya konuldu. Beyin BDNF düzeyleri SSH grubunda, diğer gruplara göre anlamlı derecede yüksek ve K grubunda ise diğer gruplara göre anlamlı derecede düşük olduğu belirlendi (p<0,001). ES ve SSH grupları serum BDNF düzeylerinin, K ve SSH+ES gruplarına göre anlamlı derecede yüksek olduğu bulundu (p<0,01). SSH grubu beyin NGF düzeyinin, K ve SSH+ES gruplarına göre anlamlı derecede yüksek olduğu belirlendi (p<0,05). SSH grubu ve SSH+ ES grubu serum NGF düzeylerinin, K ve ES gruplarına göre anlamlı derecede düşük olduğu bulundu (p<0,01). Siyatik sinir hasarı oluşturulmasından sonra elektriksel stimülasyon uygulamasının beyin BDNF düzeyinin artmasına, serum NGF düzeyinin ise azalmasına neden olduğu belirlendi. Sonuç olarak, siyatik sinir hasarı sonrası BDNF ve NGF nörotrofinlerinin salınımının ve elektriksel stimülasyonun bu salınıma etkisinin olabileceği düşünülmektedir.
Purpose of the present study was to investigate the effects of electrical stimulation on nervous system in rats with sciatic nerve injury. In this study, 28 Wistar Albino rats (2 months old, male, 250-300 g) was used. Four groups were created and each group had 7 animals; control (K) group, electrical stimulation (ES) group, sciatic nerve injury (SSH) group and sciatic nerve injury + electrical stimulation (SSH+ES) group. The experimental sciatic nerve injury model was made by compressing the sciatic nerve with Yasargil FE 693 temporary aneurysm that a closing force (50 g/cm) for 2 minutes. Electrical stimulation was applied 200 μs current time, 20 Hz frequency, 2mA amplitude for 20 minutes during 15 days. At the end of the study all rats were sacrificed. In rats with sciatic nerve damage, the effects of electrical stimulation were revealed on nerves with determination of brain derived neurotrophic factor (BDNF) and nerve growth factor (NGF) levels in serum and brain tissue by ELISA analyzes. Brain BDNF levels were determined to be significantly higher in the SSH group compared to the other groups, and significantly lower in the K group compared to the other groups (p<0.001). Serum BDNF levels in the ES and SSH groups were found to be significantly higher than the K and SSH + ES groups (p<0.01). It was determined that the brain NGF level in the SSH group was significantly higher than the K and SSH + ES groups (p<0.05). Serum NGF levels in the SSH group and the SSH + ES group were found to be significantly lower than the K and ES groups (p<0.01). It was determined that electrical stimulation application caused an increase in brain BDNF level and a decrease in serum NGF level after the sciatic nerve injury was created. In conclusion, it is thought that BDNF and NGF neurotrophins may be released after sciatic nerve injury and electrical stimulation may have an effect on this release.
Purpose of the present study was to investigate the effects of electrical stimulation on nervous system in rats with sciatic nerve injury. In this study, 28 Wistar Albino rats (2 months old, male, 250-300 g) was used. Four groups were created and each group had 7 animals; control (K) group, electrical stimulation (ES) group, sciatic nerve injury (SSH) group and sciatic nerve injury + electrical stimulation (SSH+ES) group. The experimental sciatic nerve injury model was made by compressing the sciatic nerve with Yasargil FE 693 temporary aneurysm that a closing force (50 g/cm) for 2 minutes. Electrical stimulation was applied 200 μs current time, 20 Hz frequency, 2mA amplitude for 20 minutes during 15 days. At the end of the study all rats were sacrificed. In rats with sciatic nerve damage, the effects of electrical stimulation were revealed on nerves with determination of brain derived neurotrophic factor (BDNF) and nerve growth factor (NGF) levels in serum and brain tissue by ELISA analyzes. Brain BDNF levels were determined to be significantly higher in the SSH group compared to the other groups, and significantly lower in the K group compared to the other groups (p<0.001). Serum BDNF levels in the ES and SSH groups were found to be significantly higher than the K and SSH + ES groups (p<0.01). It was determined that the brain NGF level in the SSH group was significantly higher than the K and SSH + ES groups (p<0.05). Serum NGF levels in the SSH group and the SSH + ES group were found to be significantly lower than the K and ES groups (p<0.01). It was determined that electrical stimulation application caused an increase in brain BDNF level and a decrease in serum NGF level after the sciatic nerve injury was created. In conclusion, it is thought that BDNF and NGF neurotrophins may be released after sciatic nerve injury and electrical stimulation may have an effect on this release.
Açıklama
Anahtar Kelimeler
Biyokimya, Biochemistry ; Fizyoterapi ve Rehabilitasyon
