Effects of genotype and 2,4,5-T concentrations on callus induction, shoot formation and plant regeneration from young inflorescences in orchardgrass (Dactylis glomerata L.)
[ N/A ]
Tarih
2013
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wfl Publ
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
This study was carried out to determine the effects of MS medium supplemented with 2, 4, 6 and 8 mg l(-1) of 2,4,5-T (2,4,5-trichlorophenoxyacetic acid) on young inflorescences of five ecotypes of orchardgrass (Dactylis glomerata L.). The results showed that there was a significant interaction between ecotypes and 2,4,5-T concentrations in all characteristics studied. Depending on the ecotypes, rate of callus induction (%), rate of shoot formation (%), callus weight (mg/per Petri dish) and number of regenerates per inflorescence segment varied and ranged 6.3-37.5%, 7.8-51.6%, 13.1-115.4 mg and 0.3-1.1, respectively. Rates of callus induction and shoot formation as well as callus weight were also significantly influenced by the 2,4,5-T concentrations. The explants cultured on MS medium containing 4 mg(-1) of 2,4,5-T showed highest callus induction rate of 53.8 % with callus weight of 170.4 mg/Petri dish and 1.6 shoots per explant. It was concluded that best regeneration from young inflorescence explant of orchardgrass (D. glomerata) could be obtained on MS medium containing 4 mg l(-1) of 2,4,5-T and the developed protocol will help in easy multiplication of orchard grass in future breeding studies.
Açıklama
Anahtar Kelimeler
Orchardgrass, MS medium, 2,4,5-T., tissue culture, plant regeneration
Kaynak
Journal of Food Agriculture & Environment
WoS Q Değeri
N/A
Scopus Q Değeri
N/A
Cilt
11
Sayı
1
