Effects of genotype and 2,4,5-T concentrations on callus induction, shoot formation and plant regeneration from young inflorescences in orchardgrass (Dactylis glomerata L.)

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dc.contributor.authorAtis, Ibrahim
dc.contributor.authorCan, Ersin
dc.contributor.authorCeliktas, Nafiz
dc.contributor.authorHatipoglu, Rustu
dc.date.accessioned2024-09-18T20:52:55Z
dc.date.available2024-09-18T20:52:55Z
dc.date.issued2013
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractThis study was carried out to determine the effects of MS medium supplemented with 2, 4, 6 and 8 mg l(-1) of 2,4,5-T (2,4,5-trichlorophenoxyacetic acid) on young inflorescences of five ecotypes of orchardgrass (Dactylis glomerata L.). The results showed that there was a significant interaction between ecotypes and 2,4,5-T concentrations in all characteristics studied. Depending on the ecotypes, rate of callus induction (%), rate of shoot formation (%), callus weight (mg/per Petri dish) and number of regenerates per inflorescence segment varied and ranged 6.3-37.5%, 7.8-51.6%, 13.1-115.4 mg and 0.3-1.1, respectively. Rates of callus induction and shoot formation as well as callus weight were also significantly influenced by the 2,4,5-T concentrations. The explants cultured on MS medium containing 4 mg(-1) of 2,4,5-T showed highest callus induction rate of 53.8 % with callus weight of 170.4 mg/Petri dish and 1.6 shoots per explant. It was concluded that best regeneration from young inflorescence explant of orchardgrass (D. glomerata) could be obtained on MS medium containing 4 mg l(-1) of 2,4,5-T and the developed protocol will help in easy multiplication of orchard grass in future breeding studies.en_US
dc.identifier.endpage567en_US
dc.identifier.issn1459-0255
dc.identifier.issn1459-0263
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-84874514239en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage564en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12483/11487
dc.identifier.volume11en_US
dc.identifier.wosWOS:000315888600001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherWfl Publen_US
dc.relation.ispartofJournal of Food Agriculture & Environmenten_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectOrchardgrassen_US
dc.subjectMS mediumen_US
dc.subject2,4,5-T.en_US
dc.subjecttissue cultureen_US
dc.subjectplant regenerationen_US
dc.titleEffects of genotype and 2,4,5-T concentrations on callus induction, shoot formation and plant regeneration from young inflorescences in orchardgrass (Dactylis glomerata L.)en_US
dc.typeArticleen_US

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