Pathological and microbiological investigations of pneumonic Pasteurellosis in sheep
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Tarih
2013
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info:eu-repo/semantics/openAccess
Özet
Bu çalışmada, Şanlıurfa ilinde Mart 2010-Mart 2011 tarihleri arasında, 110 adet pnömoni şüpheli akciğer dokuları histopatolojik immunohistokimyasal ve mikrobiyolojik olarak incelendi. İncelenen akciğerlerde makroskobik olarak konsolide alanların lokalizasyonu belirlendikten sonra bu bölgelerden alınan doku örneklerinin bir kısmı soğuk zincirde muhafaza edilerek mikrobiyolojik inceleme için kullanıldı. Doku örneklerinin bir kısmı %7 lik koyun kanlı agar ve Mc Conkey agara ekildi. Diğer bir kısım ise %10’luk tamponlu formaldehitte tespit edildikten sonra, rutin doku takibinden geçirilip, histopatolojik incelemeler için Hematoksilen&Eozin (H&E) ile boyandı. Histopatolojik olarak pnömoni teşhisi konulan kesitler, poliklonal tavşan Mannheimia haemolytica ve Pasteurella multocida hiperimmun serumları kullanılarak Avidin Biyotin Kompleks Peroksidaz (ABC-P) Yöntemi ile immunohistokimyasal olarak boyandı. İncelenen hayvanlarda mikrobiyolojik, histopatolojik ve immunohistokimyasal bulgular karşılaştırmalı olarak değerlendirildi. Mikrobiyolojik ekimlerde 38 vakada Pasteurella multocida pnömoni etkeni olarak belirlendi. Yapılan immunohistokimyasal boyamalar sonrası toplam 35 hayvanda Mannheimia haemolytica, 30 hayvanda Pasteurella multocida, 23 hayvanda her ikisinin birden ve 45 hayvanda ise nedeni belirlenmeyen farklı etkenlerin pnömoni sebebi olduğu ortaya konuldu. Bu çalışmanın amacı Şanlıurfa İlindeki koyun pnömonilerinde Pasteurella spp.’lerin yeri ve öneminin belirlenmesidir.
In this study, between March 2010 and March 2011, 110 pneumonia suspected lung tissues were examined histopathologically, immunohistochemically and microbiologically, in Sanliurfa province. After definition of the macroscopic localization of the consolidated areas in the lungs, tissue samples were taken and preserved in cold chain and 10% formalin for microbiological and pathological examinations, respectively. For bacteriological examination of Pasteurella spp. lung specimens were inoculated in 7% sheep blood agar and Mc Conkey agar. After routine pathological tissue follow up procedure, tissues were embedded in paraffin and obtained sections stained with Hematoxylin&Eosin (H&E). The cases, having histopathological findings consistent with pneumonia, were forwarded to immunohistochemical (IHC) examinations to know whether lesions related to Mannheimia haemolytica and Pasteurella multocida using hyperimmune polyclonal rabbit sera in Avidin Biotin Complex Peroxidase (ABC-P). Microbiological, histopathological and immunohistochemical findings were comparatively evaluated in examined animals. Pasteurella multocida as a cause of pneumonia were detected in 38 cases of microbiological inoculations. Immunohistochemical staining resulted Mannheimia haemolytica (n=35) and Pasteurella multocida (n=30) positive. Immunohistochemically both Mannheimia haemolytica and Pasteurella multocida were positive in 23 cases and 45 animals were negative for both bacteria. The aim of this study is to show importance and role of Pasteurella spp, in sheep pneumonia in Sanliurfa region.
In this study, between March 2010 and March 2011, 110 pneumonia suspected lung tissues were examined histopathologically, immunohistochemically and microbiologically, in Sanliurfa province. After definition of the macroscopic localization of the consolidated areas in the lungs, tissue samples were taken and preserved in cold chain and 10% formalin for microbiological and pathological examinations, respectively. For bacteriological examination of Pasteurella spp. lung specimens were inoculated in 7% sheep blood agar and Mc Conkey agar. After routine pathological tissue follow up procedure, tissues were embedded in paraffin and obtained sections stained with Hematoxylin&Eosin (H&E). The cases, having histopathological findings consistent with pneumonia, were forwarded to immunohistochemical (IHC) examinations to know whether lesions related to Mannheimia haemolytica and Pasteurella multocida using hyperimmune polyclonal rabbit sera in Avidin Biotin Complex Peroxidase (ABC-P). Microbiological, histopathological and immunohistochemical findings were comparatively evaluated in examined animals. Pasteurella multocida as a cause of pneumonia were detected in 38 cases of microbiological inoculations. Immunohistochemical staining resulted Mannheimia haemolytica (n=35) and Pasteurella multocida (n=30) positive. Immunohistochemically both Mannheimia haemolytica and Pasteurella multocida were positive in 23 cases and 45 animals were negative for both bacteria. The aim of this study is to show importance and role of Pasteurella spp, in sheep pneumonia in Sanliurfa region.
Açıklama
Anahtar Kelimeler
Veterinerlik
Kaynak
Kafkas Üniversitesi Veteriner Fakültesi Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
19
Sayı
1
