Oxidative stress markers in IVF patients and their association with pregnancy results
[ N/A ]
Tarih
2014
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Turkiye Klinikleri
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Objective: Oxidative stress has been well recognized as a component in the pathogenesis of many diseases, including female infertility. In this study, we aimed to investigate serum paraoxonase (PON1), arylesterase and myeloperoxidase (MPO) activities, and lipid hydroperoxide (LOOH) levels as oxidative stress markers along with their relationship with the pregnancy rate in women undergoing in vitro fertilization (IVF) treatment. Material and Methods: Serum samples of 179 subjects were collected immediately before starting ovarian stimulation and on ovum pick-up (OPU) day during IVF treatment. Stimulation protocol and starting doses were determined individually. When the leading follicle reached at least 17 mm in size, recombinant human chorionic gonadotropin (Ovitrelle, 250 mcg, Serono) was administered for ovulation induction. Serum basal/salt-stimulated paraoxonase, arylesterase activities, myeloperoxidase activity, and lipid hydroperoxide levels were compared between two samples. Subjects were also divided into two groups according to the presence of pregnancy, and the groups were compared in terms of oxidative stress markers studied. Results: Serum MPO activity and LOOH levels were significantly higher on ovum pick up day (p<0.05, p<0.001, respectively), while basal/salt-stimulated paraoxonase and arylesterase activities were significantly lower (p=0.012, p=0.041, respectively) than those before ovarian stimulation. Serum LOOH levels on OPU day were significantly higher in non-pregnant group than pregnant one (p<0.001). Although basal/salt-stimulated paraoxonase, arylesterase and myeloperoxidase activities were higher in the non-pregnant group compared to the pregnant group, it was not statistically significant (p>0.05). Conclusion: Ovarian stimulation during IVF treatment resulted in increased oxidative stress and decreased PON1 activity. However, changes in the studied parameters were not found to be significantly associated with the pregnancy results. Further studies are required to clarify our results. © 2014 by Türkiye Klinikleri.
Açıklama
Anahtar Kelimeler
Human, In vitro fertilization, Lipid peroxides, Peroxidase, PON1 protein
Kaynak
Turkiye Klinikleri Journal of Medical Sciences
WoS Q Değeri
Scopus Q Değeri
Q4
Cilt
34
Sayı
2
