Effect of Pro-Inflammatory Cytokine IL-1?, on Urotensin II Gene Expression in Human Lung Cancer Cells
[ N/A ]
Tarih
2018
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Duzce Univ
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Objective: Lung cancer is the deadliest cancer type world-wide. Poor prognosis of lung cancer patients and lack of an effective treatment require detailed understanding of lung cancer pathogenesis. It was highlighted in some studies that U-II is likely to be a biomarker or molecular target for the prevention and treatment of some diseases such as lung cancer. But its molecular action mechanism has not been elucidated yet. In the present study, we aimed to investigate the role of U-II in lung cancer. Methods: In our study, A549 cells were induced with different doses of IL-1 beta at different durations (1, 3 ng/ml; 6, 24 hours). mRNA levels of GAPDH, NF-kappa B1, MMP-1, and U-II were analyzed with RT-qPCR. The Delta Delta Ct (Delta Delta Ct) method was used for data analysis. The analyzed data were expressed as the fold-change. Results: Our results indicate that U-II gene is expressed in A549 cells and IL-1 beta can induce gene expressions of U-II, MMP-1 and NF-kappa B1 in A549 cells. Conclusions: U-II is a promising molecular target in treatment and prevention of lung cancer. Therefore, further studies are needed to enlighten molecular mechanism of U-II in lung adenocarcinoma.
Açıklama
Anahtar Kelimeler
Urotensin II, IL-1 beta, NF-kappa B1, MMP-1, Lung Cancer
Kaynak
Konuralp Tip Dergisi
WoS Q Değeri
N/A
Scopus Q Değeri
Cilt
10
Sayı
3
