Effects of ultrasonication on damaged spermatozoa and mitochondrial activity rate
dc.contributor.author | Akalın Peker, Pınar | |
dc.contributor.author | Başpınar, Nuri | |
dc.contributor.author | Çoyan, Kenan | |
dc.contributor.author | Bucak, Mustafa Numan | |
dc.contributor.author | Güngör, Şükrü | |
dc.contributor.author | Öztürk, Caner | |
dc.date.accessioned | 2019-07-16T16:00:59Z | |
dc.date.available | 2019-07-16T16:00:59Z | |
dc.date.issued | 2016 | |
dc.department | Hatay Mustafa Kemal Üniversitesi | en_US |
dc.description.abstract | The aim of this study was to describe an optimal sonication procedure for sperm cells. Therefore, we used several parameters such as damaged spermatozoa rate (%), mitochondrial activity rate (%), levels of lipid peroxidation, and total antioxidant potential. Ejaculates were collected from rams (n = 3) and were divided into aliquots and 3-, 6-, and 10-s duration times; 1, 3, 5, and 8 repetitive application groups were established. In the groups with 3-, 6- and 10-s duration times, with the increasing number of repeated applications, damaged spermatozoa rates increased (P < 0.05) while mitochondrial activity rates decreased (P < 0.05). In relation with sonication duration time, total antioxidant potential levels increased (P < 0.05) in single-application groups compared to those in control groups and gradually decreased as the repetitions increased. The most effective results were obtained in the group with 8 repetitions and 10-s duration based on damaged spermatozoa rate and mitochondrial activity rate. | en_US |
dc.description.abstract | The aim of this study was to describe an optimal sonication procedure for sperm cells. Therefore, we used several parameters such as damaged spermatozoa rate (%), mitochondrial activity rate (%), levels of lipid peroxidation, and total antioxidant potential. Ejaculates were collected from rams (n = 3) and were divided into aliquots and 3-, 6-, and 10-s duration times; 1, 3, 5, and 8 repetitive application groups were established. In the groups with 3-, 6- and 10-s duration times, with the increasing number of repeated applications, damaged spermatozoa rates increased (P < 0.05) while mitochondrial activity rates decreased (P < 0.05). In relation with sonication duration time, total antioxidant potential levels increased (P < 0.05) in single-application groups compared to those in control groups and gradually decreased as the repetitions increased. The most effective results were obtained in the group with 8 repetitions and 10-s duration based on damaged spermatozoa rate and mitochondrial activity rate. | en_US |
dc.identifier.endpage | 199 | en_US |
dc.identifier.issn | 1300-0128 | |
dc.identifier.issue | 2 | en_US |
dc.identifier.scopus | 2-s2.0-84957541238 | en_US |
dc.identifier.scopusquality | Q3 | en_US |
dc.identifier.startpage | 195 | en_US |
dc.identifier.uri | https://trdizin.gov.tr/publication/paper/detail/TWpFek16UTRPQT09 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12483/2577 | |
dc.identifier.volume | 40 | en_US |
dc.identifier.wos | WOS:000369159000011 | en_US |
dc.identifier.wosquality | Q3 | en_US |
dc.indekslendigikaynak | Web of Science | en_US |
dc.indekslendigikaynak | Scopus | en_US |
dc.indekslendigikaynak | TR-Dizin | en_US |
dc.language.iso | en | en_US |
dc.relation.ispartof | Turkish Journal of Veterinary and Animal Sciences | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US] |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | Ziraat | en_US |
dc.subject | Sütçülük ve Hayvan Bilimleri | en_US |
dc.title | Effects of ultrasonication on damaged spermatozoa and mitochondrial activity rate | en_US |
dc.type | Article | en_US |
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