Red blood cell oxidant /antioxidant status in patients with lung cancer and pneumonia
[ N/A ]
Tarih
2004
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu çalışmanın amacı akciğer kanserli ve pnömonili hastaların eritrositlerinde bazı anti-oksidan enzimlerin aktivitesini ve nitrik oksit (NO) ile malondialdehit (MDA) seviyelerini araştırmaktır. Akciğer kanserli (n=15), pnömonili (n=12) ve kontrol için sağlıklı (n=14) kişilerin eritrositlerinde süperoksit dismutaz (SOD), katalaz (CAT) ve glutatyon peroksidaz (GSH-Px) enzim aktiviteleri ile MDA ve NO seviyeleri spektrofotometrik yöntemlerle ölçüldü. Akciğer kanserli hastaların eritrosit CAT ve GSH-Px aktiviteleri pnömoni ve kontrol grubu hastaları ile karşılaştırıldığında azalmış bulundu (p<0.0001). Pnömonili hastaların SOD enzim aktivitesi, kanserli hastalarınkine göre %18, kontrol hastalarınkine göre %15 artmış bulundu. Ancak bu artış istatistiksel olarak anlamlı değildi. Akciğer kanserli grupta, MDA ile SOD arasında (r=0.796, p<0.0001); NO ile SOD arasında (r=0.759, p<0.001); SOD ile CAT arasında (r=-0.543, p<0.037) istatistiksel olarak anlamlı korelasyon bulundu. Bu sonuçlar, akciğer kanserli hastaların eritrositlerinde antioksidan enzim aktivitelerinde yetmezlik olduğu fikrini vermektedir. Bu da oksidatif stres ve hücresel hasarın artışına neden olmaktadır. Kanseröz yapılardaki DNA değişiklikleri buna örnek verilebilir. Eritrositler akciğer kanserinde oksidan/antioksidan değişikliklerin göstergesi olabilir. Akciğer kanserli ve pnömonili hasta grupları arasında NO ile SOD ve MDA ile SOD arasında pozitif korelasyon bulunmuştur.
The aim of this study was to investigate the activities of antioxidant enzymes and the levels of nitric oxide (NO) and malondialdehyde (MDA) in erythrocytes of patients with lung cancer and pneumonia. Erythrocyte superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) activities as well as MDA and NO levels were measured spectrophotometrically in patients with lung cancer (n=15) and pneumonia (n=12) as well as healthy control subjects (n=14). Erythrocyte CAT and GSH-Px activities were found to be decreased in patients with lung cancer compared with pneumonia and control groups (p<0.0001). It was shown that there was an increase in SOD activity in patients with pneumonia compared to lung cancer (18%) and control groups (15%), but the difference was not statistically significant. Significant correlations were found between MDA and SOD (r=0.796, p<0.0001); between NO and SOD (r=0.759, p<0.001); and between SOD and CAT (r=-0.543, p<0.037) in the lung cancer group. These results suggest insufficient antioxidant enzyme activities in erythrocytes of patients with lung cancer that leads to increased oxidative stress and damage to vital cellular components such as DNA with malignant changes. Erythrocytes may reflect the oxidant/antioxidant changes in lung cancer. Results have shown positive correlations between erythrocyte NO and SOD, and MDA and SOD in both lung cancer and control groups.
The aim of this study was to investigate the activities of antioxidant enzymes and the levels of nitric oxide (NO) and malondialdehyde (MDA) in erythrocytes of patients with lung cancer and pneumonia. Erythrocyte superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) activities as well as MDA and NO levels were measured spectrophotometrically in patients with lung cancer (n=15) and pneumonia (n=12) as well as healthy control subjects (n=14). Erythrocyte CAT and GSH-Px activities were found to be decreased in patients with lung cancer compared with pneumonia and control groups (p<0.0001). It was shown that there was an increase in SOD activity in patients with pneumonia compared to lung cancer (18%) and control groups (15%), but the difference was not statistically significant. Significant correlations were found between MDA and SOD (r=0.796, p<0.0001); between NO and SOD (r=0.759, p<0.001); and between SOD and CAT (r=-0.543, p<0.037) in the lung cancer group. These results suggest insufficient antioxidant enzyme activities in erythrocytes of patients with lung cancer that leads to increased oxidative stress and damage to vital cellular components such as DNA with malignant changes. Erythrocytes may reflect the oxidant/antioxidant changes in lung cancer. Results have shown positive correlations between erythrocyte NO and SOD, and MDA and SOD in both lung cancer and control groups.
Açıklama
Anahtar Kelimeler
Onkoloji
Kaynak
Türk Hematoloji Onkoloji Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
14
Sayı
4
