Neuroprotective effects of amantadine for experimental acute carbon monoxide poisoning
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Date
2022
Journal Title
Journal ISSN
Volume Title
Publisher
Verduci Publisher
Access Rights
info:eu-repo/semantics/closedAccess
Abstract
OBJECTIVE: Amantadine is known to have a neuroprotective effect in many neurolog-ical diseases. This study aims at investigating the neuroprotective effect of amantadine in rats ex-posed to carbon monoxide (CO) poisoning.MATERIALS AND METHODS: Rats were maintained under standard experimental labora-tory conditions and randomized into 4 different groups of 7 each namely control, amantadine on-ly, CO exposure, and amantadine + CO exposure. For immunohistochemical analysis, tissues tak-en from the prefrontal and hippocampal regions were taken into formalin and kept for at least one day. Afterward, the tissue was followed and blocked for paraffin blocking. N-Methyl D-Aspar-tate (NMDA) levels in homogenates were studied by the Enzyme-Linked Immunosorbent Assay (ELISA) method. Superoxide dismutase (SOD) and catalase (CAT) activities in the supernatants were studied with commercial kits. Nitric oxide (NO) and Asymmetric Dimethyl Arginine (ADMA) levels were studied by the ELISA method. En-zyme activity values were calculated by dividing the protein values in the supernatants and nor-malizing them.RESULTS: CAT, SOD, NMDA, ADMA, and NO levels were statistically significantly different between the groups (p < 0.05). According to post-hoc pairwise comparison test results, the values of the control and amantadine groups for CAT, SOD, NMDA, ADMA, and NO parameters were significantly higher than that of CO group. Similarly, values in the control and amantadine groups were considerably higher than values for the amantadine + CO group. NMDA values were significantly lower in group amantadine + CO than in CO group (p: 0.049).CONCLUSIONS: Apoptosis and endothelial damage after CO poisoning is a complex pro-cess, and amantadine administration has a limit-ed contribution in preventing this process.
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Keywords
Amantadine, Bcl-2, Carbon monoxide, Intoxication, N-Metil D-Aspartate, Neuroprotective, Poisoning, Rat
Journal or Series
European Review For Medical and Pharmacological Sciences
WoS Q Value
Q2
Scopus Q Value
Q2
Volume
26
Issue
19
