Orak hücre anemili olgularda miRNA ekspresyon düzeyleri ile klinik parametreler arasındaki korelasyonun araştırılması
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Dosyalar
Tarih
2022
Yazarlar
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Yayıncı
Hatay Mustafa Kemal Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu çalışmada, orak hücre anemili olgularda mikroRNA ve diğer kan parametrelerinin düzeyleri ölçülerek hastalığın klinik şiddetine olan etkisi araştırılmıştır. Çalışmaya 43 orak hücre anemi hastası ile yaş ve cinsiyet olarak benzer 39 sağlıklı kontrol dahil edilmiştir. Tüm bireylerden iki tüp kan alınmıştır. Bir tüpten tam kan sayımı ve HPLC yöntemi ile hemoglobin varyant analizi yapılmıştır. Diğer tüpten ise RNA izolasyonu yapılıp cDNA elde edilmiştir. Daha sonra ise RT-PCR yöntemi kullanılarak, olguların miRNA ekspresyon seviyesi ölçülmüştür. Klinik skorlama kriterlerine göre, her hastanın klinik skoru belirlenmiştir. Hastalar skorlarına göre asemptomatik, ılımlı ve ağır olmak üzere üç ana fenotip grubundan birine dahil edilmiştir. Çalışmada, hasta ve kontrol gruplarının tam kan sayımı parametreleri karşılaştırılmıştır. WBC, RBC, Hb, Hct, HbF düzeyleri hasta ve kontrol grupları arasında istatistiksel olarak önemli derecede anlamlı bulundu (p<0,001). MCV ve MCHC düzeyleri iki grup arasında istatistiksel olarak anlamlı bulundu (p=0,012, p=0,028). Bunun yanı sıra, MCH düzeyleri iki grup arasında istatiksel olarak anlamlı bulunmadı (p=0,059). Ayrıca, HbF seviyeleri ve fenotip sınıflarına göre karşılaştırıldı ve asemptomatik, ılımlı ve ağır grup arasında HbF seviyeleri istatistiksel olarak anlamlı fark bulundu (p=0,014). Hasta ve kontrol grubunun miR-326, miR-144 ve miR-150 ekspresyon düzeyleri karşılaştırıldığında hasta grupta anlamlı bir şekilde düşük eksprese edilmiştir (p=0,004, p<0.001, p=0,004). Bu sonuçlar bize; miRNA'ların orak hücre anemisi patofizyolojisinde, karmaşık düzenleyici yolaklara katkıda bulunabileceğini ve klinik heterojenitenin tanımlanmasına yardımcı olabileceğini göstermektedir.
In this study, microRNAs and other blood parameters levels of patients with sickle cell anemia were measured and their effect on the clinical severity of the disease was investigated. The study included 43 sickle cell anemia patients and 39 age- and gender-matched healthy controls. Two tubes of blood were taken from all individuals. Complete blood count with a complete blood count device from a tube and hemoglobin variant analysis with HPLC method were performed. RNA was isolated from the other tube and cDNA was obtained. Then, the miRNA expression level of the cases was measured using the RT-PCR method. According to clinical scoring criteria, the clinical score of each patient was determined. Patients were included in one of three main phenotype groups according to their scores: asymptomatic, moderate and severe. In the study, complete blood count parameters of the patient and control groups were compared. WBC, RBC, Hb, Hct, HbF levels were found to be statistically significant between the two groups (p<0.001). MCV and MCHC levels were statistically significant between the two groups (p=0.012, p=0.028). In addition, MCH levels were not statistically significant between the two groups (p=0.059). In addition, HbF levels and phenotype classes were compared, and there was a statistically significant difference in HbF levels between the asymptomatic, moderate and severe groups (p=0.014). When the expression levels of miR-326, miR-144 and miR-150 of the patient and control groups were compared, it was significantly lower in the patient group (p=0.004, p<0.001, p=0.004). These results show us; miRNAs may contribute to complex regulatory pathways in the pathophysiology of sickle cell anemia and help define clinical heterogeneity.
In this study, microRNAs and other blood parameters levels of patients with sickle cell anemia were measured and their effect on the clinical severity of the disease was investigated. The study included 43 sickle cell anemia patients and 39 age- and gender-matched healthy controls. Two tubes of blood were taken from all individuals. Complete blood count with a complete blood count device from a tube and hemoglobin variant analysis with HPLC method were performed. RNA was isolated from the other tube and cDNA was obtained. Then, the miRNA expression level of the cases was measured using the RT-PCR method. According to clinical scoring criteria, the clinical score of each patient was determined. Patients were included in one of three main phenotype groups according to their scores: asymptomatic, moderate and severe. In the study, complete blood count parameters of the patient and control groups were compared. WBC, RBC, Hb, Hct, HbF levels were found to be statistically significant between the two groups (p<0.001). MCV and MCHC levels were statistically significant between the two groups (p=0.012, p=0.028). In addition, MCH levels were not statistically significant between the two groups (p=0.059). In addition, HbF levels and phenotype classes were compared, and there was a statistically significant difference in HbF levels between the asymptomatic, moderate and severe groups (p=0.014). When the expression levels of miR-326, miR-144 and miR-150 of the patient and control groups were compared, it was significantly lower in the patient group (p=0.004, p<0.001, p=0.004). These results show us; miRNAs may contribute to complex regulatory pathways in the pathophysiology of sickle cell anemia and help define clinical heterogeneity.
Açıklama
Anahtar Kelimeler
Genetik, Genetics
