First dose optimization study on freezing Anatolian buffalo semen

Basit Öğe Kaydı
dc.authoridKaya, Ufuk/0000-0002-4805-0993
dc.authoridBastan, ilktan/0000-0001-8155-1960
dc.contributor.authorBastan, Ilktan
dc.contributor.authorSahin, Derya
dc.contributor.authorKorkmaz, Firat
dc.contributor.authorSimsek, Seher
dc.contributor.authorKaya, Ufuk
dc.contributor.authorSatilmis, Muharrem
dc.date.accessioned2024-09-18T20:06:16Z
dc.date.available2024-09-18T20:06:16Z
dc.date.issued2024
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractThe main objective of sperm production centers is to produce as many straws as possible from the obtained ejaculates using the optimal dilution rate. To this end, this study is the first to evaluate the effect of different semen extender rates on Anatolian buffalo semen quality. Ejaculates were collected by artificial vagina from three Anatolian buffalo bulls. These ejaculates were divided into three aliquots and filled into 0.25 ml straws with soy-based extenders at concentrations of 35, 25, and 15 million sperm/straw (n=105). The straw samples of different sperm concentrations were frozen. The quality of sperm was evaluated after thawing (37 degrees C, 30 sec) and following the thermoresistance test (37 degrees C, 3 h). The post-thaw total motility and progressive motility values were similar between the groups. However, following the thermoresistance test, there was a significant decrease in total motility in the 35 million sperm/straw group, and the progressive motility was significantly higher in the 25 million sperm/straw group. There was no statistically significant difference between the groups in terms of sperm kinetic parameters, except for VSL after thawing, as well as VAP and LIN values following the thermoresistance test. The overall mean PMAI and STR values were the highest in the 25 million sperm/straw group. In conclusion, it is recommended to dilute the Anatolian buffalo semen at a concentration of 25 million/0.25 ml when freezing it with a soy-based semen extender. In addition, it is considered that soy-based extenders compensate for cryo-damage to sperm motility for a short time, and the thermoresistance test should be applied for objective evaluation in dose optimization studies.en_US
dc.description.sponsorshipDirectorate of the International Center for Livestock Research and Training Institute, Ankara, Turkiye; Department of Biotechnology and Artificial Insemination, the International Center for Livestock Research and Training Instituteen_US
dc.description.sponsorshipThis study was supported by the Directorate of the International Center for Livestock Research and Training Institute, Ankara, Turkiye. The authors thank the Department of Biotechnology and Artificial Insemination, the International Center for Livestock Research and Training Institute for their support and assistance.en_US
dc.identifier.doi10.33988/auvfd.1205794
dc.identifier.endpage356en_US
dc.identifier.issn1300-0861
dc.identifier.issn1308-2817
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85201830401en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage349en_US
dc.identifier.trdizinid1249219en_US
dc.identifier.urihttps://doi.org/10.33988/auvfd.1205794
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1249219
dc.identifier.urihttps://hdl.handle.net/20.500.12483/8424
dc.identifier.volume71en_US
dc.identifier.wosWOS:001282046100002en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.publisherAnkara Univen_US
dc.relation.ispartofAnkara Universitesi Veteriner Fakultesi Dergisien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBubalis bubalisen_US
dc.subjectComputer-assisted sperm analysisen_US
dc.subjectFlow cytometryen_US
dc.subjectFrozen semenen_US
dc.subjectSemen extenderen_US
dc.titleFirst dose optimization study on freezing Anatolian buffalo semenen_US
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim
İsim:
Tam Metin / Full text
Boyut:
1.27 MB
Biçim:
Adobe Portable Document Format
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
TR-Dizin İndeksli Yayınlar Koleksiyonu