Comparison of in vitro Resistance of Wild Leishmania İsolates, Which are Resistant to Pentavalent Antimonial Compounds, Against Drugs Used in the Treatment of Leishmaniasis

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dc.authorscopusid6602284943
dc.authorscopusid56204957900
dc.authorscopusid57202031650
dc.authorscopusid57191827786
dc.authorscopusid56211674600
dc.contributor.authorÖzbilgin, Ahmet
dc.contributor.authorÇavuş, İbrahim
dc.contributor.authorKaya, Tuğba
dc.contributor.authorYıldırım, Ahmet
dc.contributor.authorHarman, Mehmet
dc.date.accessioned2024-09-19T15:46:59Z
dc.date.available2024-09-19T15:46:59Z
dc.date.issued2020
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractObjective: Meglumine antimoniate (Glucantime®) and Sodium stibogluconate (Pentostam®) are used for the treatment of cutaneous leismaniasis in Turkey. There is a reported resistance to these drugs in recent years. The aim of the present study was to compare the in vitro sensitivities of resistant Leishmania isolates against Amphotericin B, Miltefosine, Meglumine Antimoniate, Paromomycin and Sodium Stibogluconate. Methods: Five Leishmania isolates of patients with cutaneous leishmaniasis, who showed no clinical recovery despite two consecutive meglumine antimoniate treatments, which were stored in the Parasite Bank in Manisa Celal Bayar University Medical Faculty were selected. They were genotyped with Real-Time PCR using specific primers and probes to ITS1 region. Drug resistance levels of each Leishmania isolate were analysed for Amphotericin B, Miltefosine, Meglumine Antimoniate, Paromomycin, and Sodium Stibogluconate at concentrations of 500, 250, 125, 50, 25 µg/mL with XTT method and hemocytometer. Results: It was observed that the resistant Leishmania tropica isolates showed no resistance to Amphotericin B, and were sensitive to Miltefosine, Sodium Stibogluconate, Paromomycin and Meglumin Antimonate, respectively. In addition, Leishmania tropica (MHOM/AZ/1974/SAF-K27) isolate of the control group could stay viable in none of the drug concentrations of five agents in the study. Conclusion: It was determined that none of the selected resistant L. tropica isolates showed resistance to Amphotericin B and that was also shown statistically (p<0.05). The results of this study are important in guiding clinicians and researchers who conduct studies on drugs and search for new drug molecules. © 2020 Turkish Society for Parasitology.en_US
dc.identifier.doi10.4274/tpd.galenos.2019.6661
dc.identifier.endpage16en_US
dc.identifier.issn1300-6320
dc.identifier.issue1en_US
dc.identifier.pmid32212583en_US
dc.identifier.scopus2-s2.0-85082542487en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage12en_US
dc.identifier.trdizinid366948en_US
dc.identifier.urihttps://doi.org/10.4274/tpd.galenos.2019.6661
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/366948
dc.identifier.urihttps://hdl.handle.net/20.500.12483/14891
dc.identifier.volume44en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isotren_US
dc.publisherGalenos Publishing Houseen_US
dc.relation.ispartofTurkiye Parazitoloji Dergisien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCutaneous leishmaniasisen_US
dc.subjectresistanceen_US
dc.subjectresistance testsen_US
dc.subjecttreatmenten_US
dc.subjectTurkeyen_US
dc.titleComparison of in vitro Resistance of Wild Leishmania İsolates, Which are Resistant to Pentavalent Antimonial Compounds, Against Drugs Used in the Treatment of Leishmaniasisen_US
dc.title.alternativePentavalent Antimonial Bileşiklere Dirençli Vahşi Leishmania İzolatlarının Leishmaniasis Tedavisinde Kullanılan İlaçlara Karşı in vitro Dirençlerinin Karşılaştırılmasıen_US
dc.typeArticleen_US

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