Sequence variability of Hop stunt viroid isolates from stone fruits in Turkey
Yükleniyor...
Tarih
2008
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Springer
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Hop stunt viroid (HSVd), genus Hostuviroid, family Pospiviroidae is a circular single-stranded RNA 294-303 nt in size, infecting a large number of woody hosts, such as grapevine, Citrus spp. and Prunus spp. In this study, we report the identification and molecular characterization of HSVd variants isolated in Turkey from different naturally infected Prunus sources, including apricot, plum and peach. We determined the nucleotide sequences of eleven isolates and found five new sequence variants of 296 (3 variants) or 297 (2 variants) nt, comparable in length to previously known HSVd isolates. Multiple alignments and pbylogenetic analyses showed that one apricot isolate (HSVd.AP1) clustered with the recombinant P-H/cit3 group, whereas all the others (one apricot; HSVd.AP23, two plum; HSVd.PL49 and HSVd.PL278 and one peach, HSVd.PE73) clustered with the Hop group, confirming the molecular variability of HSVd isolates. The sequence variability seems to be more related to the geographical origin of the isolates than to their hosts.
Açıklama
Anahtar Kelimeler
HSVd, detection, PCR, characterization, fruit trees, sequencing, viroid
Kaynak
Journal of Plant Pathology
WoS Q DeÄŸeri
Q3
Scopus Q DeÄŸeri
Q2
Cilt
90
Sayı
1