Isolation of Methicillin Resistant (MR) Staphylococci from Chicken MeatSamples
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Dosyalar
Tarih
2021
Yazarlar
Dergi Başlığı
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Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
The aim of this study was to determine the presence of methicillin resistant staphylococci (MRS) in retail rawchicken meat samples sold in Hatay.The antimicrobial susceptibility of the isolates was tested for 13 differentantimicrobials by disc diffusion method and investigated for resistance genes encoding methicillin (mecA), tetracycline(tetM, tetK), penicillin (blaZ), macrolide (ermA, ermC), lincosamide (lnuA) and aminoglycoside [aac(6?)/aph(2??), aph(3?)?IIIa,ant(4?)?Ia] resistance via the use of polymerase chain reaction (PCR).In addition, the presence of staphylococcalenterotoxin (SE) genes was also searched by PCR. Out of 50 collected chicken meat samples, 11 (22%) MRS was isolatedand the following species were determined: S. sciuri (72.7%, 8/11) and S. epidermidis (27.3%, 3/11). While all isolates wereresistant to oxacillin, penicillin and ampicillin, various rates of resistance were observed for tetracycline (8, 72.7%),clindamycin (3, 27.3%), trimethoprim?sulfamethoxazole (2, 18.2%), erythromycin (2, 18.2%) and rifampicin (1, 9.1%).AllMRS harbored mecA gene together with blaZ.The tetM gene responsible for ribosomal protection was detected in all phenotypically tetracycline resistant isolates. ermC gene in erythromycin resistant isolates and lnuA gene in clindamycinresistant isolates were detected. None of the isolates was found to be positive for SE genes.The results of this studyindicated that contamination of retail raw chicken meat samples with MRS poses a risk to public health due to transmissionof these bacteria to humans. Additionally, this study also highlights the importance of monitoring antimicrobial resistantbacteria in animal originated foods
Açıklama
Anahtar Kelimeler
Chicken meat, Staphylococci, Methicillin resistance
Kaynak
Harran Üniversitesi Veteriner Fakültesi Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
10
Sayı
2
