Effect of supplementations of docosahexaenoic acid (DHA) into a Tris-glucose based extender on the post-thaw sperm quality, fertility and hatching rates in brown trout (Salmo trutta macrostigma) following cryopreservation

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dc.authoridBOZKURT, Yusuf/0000-0002-6162-2466
dc.contributor.authorBozkurt, Yusuf
dc.contributor.authorYavas, Ilker
dc.date.accessioned2024-09-18T20:13:35Z
dc.date.available2024-09-18T20:13:35Z
dc.date.issued2021
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractThe supplementation of extenders with PUFAs has not been investigated in fish by now. Thus, the present study examined whether different levels of docosahexaenoic (DHA), which is the predominant PUFAs in the sperm plasma membrane, in Tris-glucose based extender could increase the quality and fertilizing ability of brown trout (Salmo trutta macrostigma) sperm following cryopreservation were evaluated. Sperm samples diluted (1:10) with the base extender were supplemented with 1, 2.5, 5, 7.5, and 10 ng mL(-1) concentrations of DHA. In the control group, sperm was diluted in the base extender, without DHA. Following dilution, the sperm was aspirated into 0.25 mL straws, frozen 3 cm of above of the liquid nitrogen (LN2) surface, and plunged directly into the LN2. Based on the results, increasing of DHA supplementations in the Tris-glucose based extender improved post-thaw progressive motility, progressive duration of motility, viability, and fertility compared to the control group (P<0.05). Additionally, the results indicated that supplementation of 7.5 ng mL(-1) of DHA significantly exhibited the best cryoprotective effect in terms of progressive motility (62.4 +/- 1.73%), progressive duration of motility (46.8 +/- 1.32s), viability (56.2 +/- 1.20%), fertility (52.6 +/- 1.48%), and hatching (28.6 +/- 1.45%). The results showed that supplementation of DHA to the Tris-glucose based extender can be beneficial for the cryopreservation of brown trout sperm.en_US
dc.description.sponsorshipIskenderun Technical University Scientific Research Fund [ISTE BAP-2017-YP-04]en_US
dc.description.sponsorshipThe authors thank the staff of the Kent Trout Aquaculture Farm, Gaziantep, Turkey. This research was partially funded by Iskenderun Technical University Scientific Research Fund (Project No: ISTE BAP-2017-YP-04).en_US
dc.identifier.doi10.22034/IAR.2021.1926852.1151
dc.identifier.endpage154en_US
dc.identifier.issn2008-4935
dc.identifier.issn2008-6970
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-85110144601en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage147en_US
dc.identifier.urihttps://doi.org/10.22034/IAR.2021.1926852.1151
dc.identifier.urihttps://hdl.handle.net/20.500.12483/9271
dc.identifier.volume13en_US
dc.identifier.wosWOS:000668156000006en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherIslamic Azad Univ, Tonekabon Branchen_US
dc.relation.ispartofInternational Aquatic Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectcryopreservationen_US
dc.subjectSpermen_US
dc.subjectExtenderen_US
dc.subjectDHAen_US
dc.subjectBrown trouten_US
dc.titleEffect of supplementations of docosahexaenoic acid (DHA) into a Tris-glucose based extender on the post-thaw sperm quality, fertility and hatching rates in brown trout (Salmo trutta macrostigma) following cryopreservationen_US
dc.typeArticleen_US

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