Comparison of conventional and polymerase chain reaction diagnostic techniques for leishmaniasis in the endemic region of Adana, Turkey

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dc.authoridUzun, Soner/0000-0001-7059-5474
dc.contributor.authorCulha, G
dc.contributor.authorUzun, S
dc.contributor.authorOzcan, K
dc.contributor.authorMemisoglu, HR
dc.contributor.authorChang, KP
dc.date.accessioned2024-09-18T21:05:16Z
dc.date.available2024-09-18T21:05:16Z
dc.date.issued2006
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractBackground Cutaneous leishmaniasis (CL) has long been reported in the Cukurova region. We have compared the sensitivity of the conventional methods of diagnosis by microscopy and cultivation of lesion aspirates against polymerase chain reaction (PCR) amplification of parasite-specific DNA from these samples. Methods The samples (n = 25) were obtained from patients clinically diagnosed with CL at the regional dermatology clinic. Aliquots of the samples were stained with Giemsa for microscopy and cultured in Novy-Nicolle-McNeal (NNN) blood agar for promastigote growth. The remainder were subjected to DNA extraction for PCR amplification of the conserved region of kinetoplast minicircle DNA. PCR products of the expected size (120 bp) were observed after agarose gel electrophoresis, followed by staining with ethidium bromide. Results The positive rates from 25 samples were 44%, 68%, and 100% for cultivation, microscopy, and PCR, respectively. Conclusions The PCR method used appears to be the most sensitive for the diagnosis of CL in this region.en_US
dc.identifier.doi10.1111/j.1365-4632.2006.02695.x
dc.identifier.endpage572en_US
dc.identifier.issn0011-9059
dc.identifier.issue5en_US
dc.identifier.pmid16700794en_US
dc.identifier.scopus2-s2.0-33646193754en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage569en_US
dc.identifier.urihttps://doi.org/10.1111/j.1365-4632.2006.02695.x
dc.identifier.urihttps://hdl.handle.net/20.500.12483/13485
dc.identifier.volume45en_US
dc.identifier.wosWOS:000237181800016en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherBlackwell Publishingen_US
dc.relation.ispartofInternational Journal of Dermatologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCutaneous Leishmaniasisen_US
dc.subjectClinical Specimensen_US
dc.subjectBiopsiesen_US
dc.subjectDnaen_US
dc.titleComparison of conventional and polymerase chain reaction diagnostic techniques for leishmaniasis in the endemic region of Adana, Turkeyen_US
dc.typeArticleen_US

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