Apoptotic and Anti-inflammatory Effects of Hypericum Perforatum Extract inHuman Basal Cell Carcinoma TE 354.T Cell Line

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dc.contributor.authorCelık, Ebru
dc.contributor.authorKaplan, Halil Mahir
dc.contributor.authorŞingirik, Ergin
dc.contributor.authorÇelik, Muhammed Salih
dc.contributor.authorAlp, Harun
dc.date.accessioned2024-09-19T16:26:50Z
dc.date.available2024-09-19T16:26:50Z
dc.date.issued2021
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractObjectives: Basal cell carcinoma is the most common malignancy in in the Caucasian race and is a serious health problem with its ever-increasing incidence and high cost. Although the treatment is surgical, some elderly patients do not accept surgical intervention. In this case, non-surgical treatments come into prominence. The Hypericum perforatum (HP) / St. John's Wort plant has been used in the treatment of many diseases for centuries among local people. Here, we aimed to aimed to elucidate the effect and molecular mechanism of the HP plant on BCC cells.Methods: Human BCC cell lines TE 354.T were acquired from ATCC®, then were cultured in Dulbecco's Modified Eagle's Medium. BCC cells were seeded at a concentration of 2x106cells in each flask (n=12). After incubation, while six of the flasks were applied with a 0.8% HP extract and 99.2% medium for 48 hours, no application was made to the control group. The effects of HP on mitochondrial mediated intrinsic and extrinsic apoptotic pathway agents, cell cycle G2 / M checkpoint kinases and inflammatory mediators were investigated in BCC cancer cells. The expression and activities of these mediators in both groups were evaluated by ELISA test. Results: It was determined that HP extract treatment increased the expression of apoptotic proteins (AIF, GADD153, GRP78, caspase-3, Bax) and cell cycle G2 / M checkpoint kinase (Wee1), though it reduced antiapoptotic protein (Bcl-2) and inflammatory mediators (iNOS, COX-2, cPLA2, NFkB) in BCC cell lines.Conclusion: HP extract can support routine chemotherapy on BCC cells with its apoptotic, anti-inflammatory and anti-cancer effect, and may be a beacon of hope in reducing resistance to chemotherapeutic drugs in the treatment of BCC.en_US
dc.identifier.doi10.5798/dicletip.887378
dc.identifier.endpage98en_US
dc.identifier.issn1300-2945
dc.identifier.issn1308-9889
dc.identifier.issue1en_US
dc.identifier.startpage92en_US
dc.identifier.trdizinid409602en_US
dc.identifier.urihttps://doi.org/10.5798/dicletip.887378
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/409602
dc.identifier.urihttps://hdl.handle.net/20.500.12483/16404
dc.identifier.volume48en_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.relation.ispartofDicle Tıp Dergisien_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.titleApoptotic and Anti-inflammatory Effects of Hypericum Perforatum Extract inHuman Basal Cell Carcinoma TE 354.T Cell Lineen_US
dc.typeArticleen_US

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