Obstrüktif uyku apne sendromu hastalarında Beclin-1 ve hipoksi ile indüklenen faktör-1 düzeylerinin araştırılması
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Dosyalar
Tarih
2022
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Yayıncı
Hatay Mustafa Kemal Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Obstrüktif uyku apne sendromu (OUAS), uyku sırasında tekrarlayan üst solunum yolunun tam veya parsiyel obstrüksiyonları ile ortaya çıkan ve sıklıkla buna eşlik eden kan oksijen satürasyonlarında (SpO2) azalma ve uyanmalar ile karakterize olan bir sendromdur. Bu çalışmada amacımız OUAS olan hastalarda Beclin-1, HIF-1α, inflamatuar ve oksidatif stres markırlarının düzeylerinin belirlenmesi, CPAP tedavisinin otofajik, hipoksik ve oksidatif stres parametreleri üzerine olan etkisini incelemek ve bu parametrelerin klinik ile olan korelasyonunu ortaya koymaktır. Çalışmaya Mustafa Kemal Üniversitesi Tayfur Ata Sökmen Tıp Fakültesi Sağlık Araştırma ve Uygulama Hastanesi Göğüs Hastalıkları Polikliniği'ne başvuran 18-69 yaş arasında 27 kontrol, 42 hafif-orta şiddette OUAS tanısı alan hasta ve 28 ağır OUAS tanısı alan hasta olmak üzere toplam 97 kişi dahil edildi. Ağır OUAS hastalarında CPAP tedavi öncesi ve CPAP tedavisinin üçüncü ayında sonuçlar kaydedildi. Kan örneklerinde Beclin-1, HIF-1α, TNF-α ve IL-6 düzeyleri ELİSA yöntemiyle, TAS (Total Antioksidan Status) ve TOS (Total Oksidan Status) parametreleri ise kolorimetrik yöntemle çalışıldı. Oksidatif Stres İndeksi (OSİ) TOS/TAS*100 şeklinde hesaplandı. Veriler için analizler IBM SPSS versiyon 22.0 ile yapıldı. Genel olarak istatistiksel anlamlılık düzeyi p<0.05 olarak ele alınırken, Bonferroni düzeltmeli Mann Whitney U Test için anlamlılık düzeyi p<0.02 olarak ele alındı. Kontrol grubu ile hafif-orta ve ağır OUAS grubunun TNF-α, IL-6, Beclin-1, HIF-1α, CRP, TOS ve OSİ parametrelerinin değerleri arasında anlamlı fark saptanmıştır. Hafif-orta OUAS ve ağır OUAS hastalarının TNF-α, IL-6, Beclin-1, HIF-1α, CRP, TOS ve OSİ değerleri kontrol grubu değerlerinden anlamlı derecede daha yüksek olduğu tespit edilmiştir. OUAS grupları arasında serum TAS düzeyleri bakımından anlamlı düzeyde fark bulunmamıştır. Ağır OUAS hastalarında tedaviden 3 ay sonraki TNF-α, IL-6, HIF-1α, CRP, TOS ve OSİ değerlerinin tedavi öncesine göre istatistiksel olarak anlamlı derecede düşük düzeylerde olduğu tespit edilmiştir. Ağır OUAS hastalarında tedaviden 3 ay sonraki Beclin-1 değerleri ise tedavi öncesine göre anlamlı bulunmamıştır. Obstrüktif uyku apne sendromu hastalarında CPAP tedavisinin Beclin-1 düzeyleri üzerine olan etkisi, Beclin-1 ve HIF-1α'nın oksidatif stres ile olan ilişkisi ilk kez çalışılmıştır. HIF-1α düzeylerinin intermitant hipoksi (İH), proinflamatuar markırlar ve oksidatif stres ile ilişkili olduğu; hastalık patogenezinde rol oynadığı ve diagnostik bir belirteç olabileceği düşünülmektedir. Ayrıca OUAS'ta otofajik yolakların etkilendiği ve hastalık patogeneziyle ilişkili olabileceği söylenebilir.
Obstructive sleep apnea syndrome (OSAS) is a syndrome that occurs with recurrent complete or partial obstructions of the upper airway during sleep and is characterized by a decrease in blood oxygen saturation (SpO2) and awakenings often accompanying this. In this study, our aim was to determine the levels of Beclin-1, HIF-1α, inflammatory and oxidative stress markers in patients with OSAS, to examine the effect of CPAP treatment on autophagic, hypoxic and oxidative stress parameters, and to reveal the correlation of these parameters with clinical. A total of 97 people, 27 controls, 42 patients diagnosed with mild-moderate OSAS, and 28 patients diagnosed with severe OSAS, between the ages of 18-69, at Mustafa Kemal University Tayfur Ata Sökmen Medical Faculty Health Research and Practice Hospital Chest Diseases Outpatient Clinic were included in the study. Results were recorded before CPAP treatment and at the third month of CPAP treatment in severe OSAS patients. Beclin-1, HIF-1α, TNF-α and IL-6 levels in blood samples were studied by ELISA method, and TAS (Total Antioxidant Status) and TOS (Total Oxidant Status) parameters were studied by colorimetric method. Oxidative Stress Index (OSI) was calculated as TOS/TAS*100. Analyzes for data were performed with IBM SPSS version 22.0. While the statistical significance level was considered as p<0.05 in general, the significance level was considered as p<0.02 for the Bonferroni corrected Mann Whitney U Test. A significant difference was found between the values of TNF-α, IL-6, Beclin-1, HIF-1α, CRP, TOS and OSI parameters of the control group and mild-moderate and severe OSAS groups. TNF-α, IL-6, Beclin-1, HIF 1α, CRP, TOS and OSI values of patients with mild-moderate OSAS and severe OSAS were found to be significantly higher than the values of the control group. There was no significant difference between the OSAS groups in terms of serum TAS levels. In severe OSAS patients, TNF-α, IL-6, HIF-1α, CRP, TOS and OSI values 3 months after treatment were found to be statistically significantly lower than before treatment. In severe OSAS patients, Beclin-1 values 3 months after treatment were not found to be significant compared to before treatment. The effect of CPAP treatment on Beclin-1 levels in patients with obstructive sleep apnea syndrome and the relationship between Beclin-1 and HIF-1α and oxidative stress were studied for the first time. HIF-1α levels are associated with intermittent hypoxia (IH), proinflammatory markers and oxidative stress; it is thought to play a role in the pathogenesis of the disease and may be a diagnostic marker. In addition, it can be said that autophagic pathways are affected in OSAS and may be related to the pathogenesis of the disease.
Obstructive sleep apnea syndrome (OSAS) is a syndrome that occurs with recurrent complete or partial obstructions of the upper airway during sleep and is characterized by a decrease in blood oxygen saturation (SpO2) and awakenings often accompanying this. In this study, our aim was to determine the levels of Beclin-1, HIF-1α, inflammatory and oxidative stress markers in patients with OSAS, to examine the effect of CPAP treatment on autophagic, hypoxic and oxidative stress parameters, and to reveal the correlation of these parameters with clinical. A total of 97 people, 27 controls, 42 patients diagnosed with mild-moderate OSAS, and 28 patients diagnosed with severe OSAS, between the ages of 18-69, at Mustafa Kemal University Tayfur Ata Sökmen Medical Faculty Health Research and Practice Hospital Chest Diseases Outpatient Clinic were included in the study. Results were recorded before CPAP treatment and at the third month of CPAP treatment in severe OSAS patients. Beclin-1, HIF-1α, TNF-α and IL-6 levels in blood samples were studied by ELISA method, and TAS (Total Antioxidant Status) and TOS (Total Oxidant Status) parameters were studied by colorimetric method. Oxidative Stress Index (OSI) was calculated as TOS/TAS*100. Analyzes for data were performed with IBM SPSS version 22.0. While the statistical significance level was considered as p<0.05 in general, the significance level was considered as p<0.02 for the Bonferroni corrected Mann Whitney U Test. A significant difference was found between the values of TNF-α, IL-6, Beclin-1, HIF-1α, CRP, TOS and OSI parameters of the control group and mild-moderate and severe OSAS groups. TNF-α, IL-6, Beclin-1, HIF 1α, CRP, TOS and OSI values of patients with mild-moderate OSAS and severe OSAS were found to be significantly higher than the values of the control group. There was no significant difference between the OSAS groups in terms of serum TAS levels. In severe OSAS patients, TNF-α, IL-6, HIF-1α, CRP, TOS and OSI values 3 months after treatment were found to be statistically significantly lower than before treatment. In severe OSAS patients, Beclin-1 values 3 months after treatment were not found to be significant compared to before treatment. The effect of CPAP treatment on Beclin-1 levels in patients with obstructive sleep apnea syndrome and the relationship between Beclin-1 and HIF-1α and oxidative stress were studied for the first time. HIF-1α levels are associated with intermittent hypoxia (IH), proinflammatory markers and oxidative stress; it is thought to play a role in the pathogenesis of the disease and may be a diagnostic marker. In addition, it can be said that autophagic pathways are affected in OSAS and may be related to the pathogenesis of the disease.
Açıklama
Anahtar Kelimeler
Biyokimya, Biochemistry, OUAS, Oksidatif Stres, İnflamasyon, Beclin-1, HIF-1, OSAS, Oxidative Stress, Inflammation, Beclin-1, HIF-1
