Antioxidant Effects of Myrtus communis L.’s Essential Oils in BEAS2B Cells Induced by Oxidative Stress with Hydrogen Peroxide

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dc.contributor.authorUlutaş, Hayriye Zehra
dc.contributor.authorDuran, Gülay Gülbol
dc.date.accessioned2024-09-19T16:23:30Z
dc.date.available2024-09-19T16:23:30Z
dc.date.issued2020
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractAim: In this study, the effects of Myrtus communis L. essential oil on the human bronchial epithelial cell line (BEAS-2B) exposed to oxidative stress with hydrogen peroxide were investigated and their effects on apoptotic pathways. Materials and Methods: The MTT ?3- (4,5-Dimethylthiazol-2-yl) -2,5-Diphenyltetrazolium Bromide? method was used to determine the appropriate doses of hydrogen peroxide ${H_2}{O_2}$ and M. communis L.’s essential oil in BEAS-2B cells. Oxidative stress formation was determined by measuring malondialdehyde (MDA) level. The cells were divided into three groups: the group exposed to oxidative stress (group with H2O2), the treatment group (${H_2}{O_2}$+ M. communis L.’s essential oil) and the control group. MDA levels were measured in all three groups and expression levels ofCaspase 3, Caspase 8, Caspase 9 and p21 genes were determined by RT-PCR method in order to detect apoptotic effects. Results: According to MTT test results, the appropriate doses were 40 ?M for ${H_2}{O_2}$and 15.625 ?g/ml for M. communis L.’s essential oil. MDA levels were significantly increased in the group treated with 40 ?M H2O2 when compared with the healthy cell group (p=0.0005). In the group treated with essential oil of M. communis L., MDA level was found similar to the control group (p>0.05). Expression levels of Caspase 3, Caspase 8 and p21 genes were significantly increased in cells where ${H_2}{O_2}$ was adminisrated at 40 ?M concentrations compared to healthy cell group (p=0.001, p=0.017 and p=0.0003, respectively). However, Caspase 9 gene expression level did not change significantly (p=0.8). Compared to the group in which the oxidative stress model was established, it was found that Caspase 3 gene expression level decreased significantly in the cells treated with M. communis L.’s oil (p=0.00007). Conclusion: In our study, it was shown that the essential oil of M. communis L. strongly decreased MDA levels and also had the potential to be a therapeutic agent due to its apoptotic inhibiting effect. M. communis L. has a strong antioxidant effect and is thought to be effective in stopping apoptosis caused by oxidative stress.en_US
dc.identifier.doi10.30565/medalanya.607989
dc.identifier.endpage28en_US
dc.identifier.issn2587-0319
dc.identifier.issue1en_US
dc.identifier.startpage21en_US
dc.identifier.trdizinid471336en_US
dc.identifier.urihttps://doi.org/10.30565/medalanya.607989
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/471336
dc.identifier.urihttps://hdl.handle.net/20.500.12483/15919
dc.identifier.volume4en_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.relation.ispartofActa Medica Alanyaen_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.titleAntioxidant Effects of Myrtus communis L.’s Essential Oils in BEAS2B Cells Induced by Oxidative Stress with Hydrogen Peroxideen_US
dc.typeArticleen_US

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